STIP1 (NM_001282652) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HEL-S-94n; HOP; IEF-SSP-3521; P60; STI1; STI1L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239010 representing NM_001282652 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCGGCAGCCCAATGGGAGAGGTGGAAATTTCCAGAACGATCAGAACCAATGGGCGCGGCCAGC GCGGCTACGATTGGCAGTGCAAAAGACCAATCCGTGTCGCAGAAGTTCGCTCCTCCCTCCATTCGTGGAG CCTGAGATGGGTCAATGAGCTGAAGGAGAAAGGCAACAAGGCCCTGAGCGTGGGTAACATCGATGATGCC TTACAGTGCTACTCCGAAGCTATTAAGCTGGATCCCCACAACCACGTGCTGTACAGCAACCGTTCTGCTG CCTATGCCAAGAAAGGAGACTACCAGAAGGCTTATGAGGATGGCTGCAAGACTGTCGACCTAAAGCCTGA CTGGGGCAAGGGCTATTCACGAAAAGCAGCAGCTCTAGAGTTCTTAAACCGCTTTGAAGAAGCCAAGCGA ACCTATGAGGAGGGCTTAAAACACGAGGCAAATAACCCTCAACTGAAAGAGGGTTTACAGAATATGGAGG CCAGGTTGGCAGAGAGAAAATTCATGAACCCTTTCAACATGCCTAATCTGTATCAGAAGTTGGAGAGTGA TCCCAGGACAAGGACACTACTCAGTGATCCTACCTACCGGGAGCTGATAGAGCAGCTACGAAACAAGCCT TCTGACCTGGGCACGAAACTACAAGATCCCCGGATCATGACCACTCTCAGCGTCCTCCTTGGGGTCGATC TGGGCAGTATGGATGAGGAGGAAGAGATTGCAACACCTCCACCACCACCCCCTCCCAAAAAGGAGACCAA GCCAGAGCCAATGGAAGAAGATCTTCCAGAGAATAAGAAGCAGGCACTGAAAGAAAAAGAGCTGGGGAAC GATGCCTACAAGAAGAAAGACTTTGACACAGCCTTGAAGCATTACGACAAAGCCAAGGAGCTGGACCCCA CTAACATGACTTACATTACCAATCAAGCAGCGGTATACTTTGAAAAGGGCGACTACAATAAGTGCCGGGA GCTTTGTGAGAAGGCCATTGAAGTGGGGAGAGAAAACCGAGAAGACTATCGACAGATTGCCAAAGCATAT GCTCGAATTGGCAACTCCTACTTCAAAGAAGAAAAGTACAAGGATGCCATCCATTTCTATAACAAGTCTC TGGCAGAGCACCGAACCCCAGATGTGCTCAAGAAATGCCAGCAGGCAGAGAAAATCCTGAAGGAGCAAGA GCGGCTGGCCTACATAAACCCCGACCTGGCTTTGGAGGAGAAGAACAAAGGCAACGAGTGTTTTCAGAAA GGGGACTATCCCCAGGCCATGAAGCATTATACAGAAGCCATCAAAAGGAACCCGAAAGATGCCAAATTAT ACAGCAATCGAGCTGCCTGCTACACCAAACTCCTGGAGTTCCAGCTGGCACTCAAGGACTGTGAGGAATG TATCCAGCTGGAGCCGACCTTCATCAAGGGTTATACACGGAAAGCCGCTGCGCTGGAAGCGATGAAGGAC TACACCAAAGCCATGGATGTGTACCAGAAGGCGCTAGACCTGGACTCCAGCTGTAAGGAGGCGGCAGACG GCTACCAGCGCTGTATGATGGCGCAGTACAACCGGCACGACAGCCCCGAAGATGTGAAGCGACGAGCCAT GGCCGACCCTGAGGTGCAGCAGATCATGAGTGACCCAGCCATGCGCCTTATCCTGGAACAGATGCAGAAG GACCCCCAGGCACTCAGCGAACACTTAAAGAATCCTGTAATAGCACAGAAGATCCAGAAGCTGATGGATG TGGGTCTGATTGCAATTCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239010 representing NM_001282652 Red=Cloning site Green=Tags(s) MESGSPMGEVEISRTIRTNGRGQRGYDWQCKRPIRVAEVRSSLHSWSLRWVNELKEKGNKALSVGNIDDA LQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKR TYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKP SDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGN DAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAY ARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQK GDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKD YTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQK DPQALSEHLKNPVIAQKIQKLMDVGLIAIR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282652
ORF Size	1770 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282652.1, NP_001269581.1
RefSeq Size	2766 bp
RefSeq ORF	1773 bp
Locus ID	10963
UniProt ID	P31948
Protein Families	Stem cell - Pluripotency
Protein Pathways	Prion diseases
MW	68.5 kDa
Gene Summary	STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009]

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