DNA polymerase mu (POLM) (NM_001284331) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Pol Mu; Tdt-N
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238341 representing NM_001284331 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCCCAAACGGCGGCGAGCGCGGGTCGGGTCCCCTAGCGGCGATGCCGCTTCCTCCACGCCGCCCT CGACGCGCTTCCCGGGAGTCGCCATCTACCTGGTCGAGCCTCGCATGGGTCGCAGCCGCCGGGCCTTCCT CACAGGCCTGGCGCGCTCCAAAGGCTTCCGCGTCCTTGACGCCTGCAGCTCCGAAGCGACACATGTTGTG ATGGAAGAGACCTCAGCAGAGGAGGCCGTCAGCTGGCAGGAGCGCAGGATGGCAGCTGCTCCCCCGGGTT GCACCCCCCCAGCTCTGCTGGACATAAGCTGGTTAACAGAGAGCCTGGGAGCTGGGCAGCCTGTACCTGT GGAGTGCCGGCACCGCCTGGAGGTGGCTGGGCCAAGGAAGGGGCCTCTGAGCCCAGCATGGATGCCTGCC TATGCCTGCCAGCGCCCTACGCCCCTCACACACCACAACACTGGCCTCTCCGAGGCTCTGGAGATACTGG CCGAGGCAGCAGGCTTTGAAGGCAGTGAGGGCCGCCTCCTCACCTTCTGCAGAGCAGCCTCGGTGCTCAA GGCCCTTCCCAGCCCTGTCACAACCCTGAGCCAGCTGCAGGGGCTTCCCCACTTTGGAGAACACTCCTCT AGGGTTGTCCAGGAGCTGCTGGAGCATGGAGTGTGTGAGGAGGTGGAGAGAGTTCGGCGCTCAGAGAGGT ACCAGACCATGAAGCTCTTCACCCAGATCTTCGGGGTCGGTGTGAAGACTGCTGACCGGTGGTACCGGGA AGGACTGCGAACCTTAGATGACCTCCGAGAGCAGCCCCAGAAACTAACCCAACAGCAGAAAGCGGCACCA CCAGGACCTGAGCACCCCAGTCCTGCGGTCCGATGTAGATGCCCTGCAGCAGGTGGTGGAGGAAGCTGTG GGGCAGGCCCTGCCTGGGGCCACCGTCACGCTGACCGGCGGCTTCCGCAGGGCCTCATCCTGTACCACCA GCACCAGCACAGCTGCTGTGAGTCCCCTACCCGCCTGGCCCAACAGAGCCACATGGACGCTTTTGAGAGA AGTTTCTGCATTTTCCGCCTACCACAACCTCCAGGGGCTGCTGTGGGGGGATCCACGAGGCCCTGCCCAT CCTGGAAGGCCGTGAGAGTGGACTTGGTAGTTGCACCCGTCAGCCAGTTCCCTTTCGCCCTGCTCGGTTG GACTGGCTCCAAGCTTTTCCAGCGGGAGCTGCGCCGCTTCAGCCGGAAGGAGAAGGGCCTGTGGCTGAAC AGCCATGGGCTGTTTGACCCGGAGCAGAAGACATTTTTCCAAGCGGCTTCAGAGGAAGACATCTTCAGAC ACCTGGGCCTTGAGTACCTTCCTCCAGAGCAGAGAAACGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238341 representing NM_001284331 Red=Cloning site Green=Tags(s) MLPKRRRARVGSPSGDAASSTPPSTRFPGVAIYLVEPRMGRSRRAFLTGLARSKGFRVLDACSSEATHVV MEETSAEEAVSWQERRMAAAPPGCTPPALLDISWLTESLGAGQPVPVECRHRLEVAGPRKGPLSPAWMPA YACQRPTPLTHHNTGLSEALEILAEAAGFEGSEGRLLTFCRAASVLKALPSPVTTLSQLQGLPHFGEHSS RVVQELLEHGVCEEVERVRRSERYQTMKLFTQIFGVGVKTADRWYREGLRTLDDLREQPQKLTQQQKAAP PGPEHPSPAVRCRCPAAGGGGSCGAGPAWGHRHADRRLPQGLILYHQHQHSCCESPTRLAQQSHMDAFER SFCIFRLPQPPGAAVGGSTRPCPSWKAVRVDLVVAPVSQFPFALLGWTGSKLFQRELRRFSRKEKGLWLN SHGLFDPEQKTFFQAASEEDIFRHLGLEYLPPEQRNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284331
ORF Size	1371 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284331.1, NP_001271260.1
RefSeq Size	2714 bp
RefSeq ORF	1374 bp
Locus ID	27434
UniProt ID	Q9NP87
Protein Families	Druggable Genome
Protein Pathways	Non-homologous end-joining
MW	50.9 kDa
Gene Summary	Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.[UniProtKB/Swiss-Prot Function]

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