Synaptotagmin VII (SYT7) (NM_001300773) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	IPCA-7; IPCA7; PCANAP7; SYT-VII; SYTVII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238288 representing NM_001300773 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCGGGACCCGGAGGCGGCCAGCCCAGGGGCGCCCTCGCGCGACGTCCTGCTGGTCTCTGCCATCA TCACCGTCAGCCTTAGCGTCACTGTCGTCCTCTGCGGCCTCTGCCACTGGTGTCAGCGCAAACTGGGCAA ACGCTACAAGAATTCCTTGGAGACGGTGGGCACGCCAGACTCAGGGCGTGGGCGCAGTGAGAAGAAGGCT ATCAATGATCTAGACAGAGACTTTTGGAATAACAATGAGAGCACAGTGCAGCAGAAATGGAGCTCCTACC CTCCCAAGGAGTTTATTCTAAACATTTCACCCTACGCCCCTTATGGCGACCCACGACTGTCCCTCAAGTT GCCTGCAGGAGGGAAGGCGGTGAACACAGCCCCCGTGCCAGGCCAGACACCCCACGATGAGTCCGACCGC CGGACCGAGCCACGTTCCTCCGTCTCAGACCTCGTCAACTCCCTCACCAGCGAGATGCTCATGCTCTCCC CAGGCTCCGAGGAGGATGAGGCCCACGAGGGTTGCAGCCGAGAGAACCTGGGCCGGATCCAGTTCAGTGT CGGCTACAACTTCCAGGAGTCCACGCTCACCGTGAAGATCATGAAGGCCCAGGAGCTGCCGGCCAAGGAC TTCAGCGGCACCAGCGACCCCTTCGTCAAGATCTACCTGCTGCCCGACAAGAAGCACAAGCTGGAGACCA AGGTGAAGCGGAAGAACCTGAACCCCCACTGGAACGAGACCTTCCTCTTTGAAGGTTTTCCCTATGAGAA GGTGGTGCAGAGGATCCTCTACCTCCAAGTCCTGGACTATGACCGCTTCAGCCGCAACGACCCCATTGGG GAGGTGTCCATCCCCCTTAACAAGGTGGACCTGACCCAGATGCAGACCTTCTGGAAGGATCTGAAGCCAT GCAGCGATGGGAGTGGGAGCCGAGGGGAGCTGCTCTTGTCTCTCTGCTACAACCCCTCTGCCAACTCCAT CATCGTGAACATCATCAAAGCCCGGAACCTCAAAGCCATGGACATCGGGGGCACATCAGACCCCTACGTG AAGGTATGGCTGATGTACAAGGACAAGCGGGTGGAGAAGAAGAAGACGGTGACGATGAAGAGGAACCTGA ACCCCATCTTCAATGAGTCCTTCGCCTTCGATATCCCCACGGAGAAGCTGAGGGAGACGACCATCATCAT CACTGTCATGGACAAGGACAAGCTCAGCCGCAATGACGTCATCGGCAAGATCTACCTGTCCTGGAAGAGC GGGCCAGGGGAGGTGAAGCACTGGAAGGACATGATTGCCCGTCCCCGGCAGCCCGTGGCCCAGTGGCACC AGCTGAAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238288 representing NM_001300773 Red=Cloning site Green=Tags(s) MYRDPEAASPGAPSRDVLLVSAIITVSLSVTVVLCGLCHWCQRKLGKRYKNSLETVGTPDSGRGRSEKKA INDLDRDFWNNNESTVQQKWSSYPPKEFILNISPYAPYGDPRLSLKLPAGGKAVNTAPVPGQTPHDESDR RTEPRSSVSDLVNSLTSEMLMLSPGSEEDEAHEGCSRENLGRIQFSVGYNFQESTLTVKIMKAQELPAKD FSGTSDPFVKIYLLPDKKHKLETKVKRKNLNPHWNETFLFEGFPYEKVVQRILYLQVLDYDRFSRNDPIG EVSIPLNKVDLTQMQTFWKDLKPCSDGSGSRGELLLSLCYNPSANSIIVNIIKARNLKAMDIGGTSDPYV KVWLMYKDKRVEKKKTVTMKRNLNPIFNESFAFDIPTEKLRETTIIITVMDKDKLSRNDVIGKIYLSWKS GPGEVKHWKDMIARPRQPVAQWHQLKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300773
ORF Size	1341 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300773.1, NP_001287702.1
RefSeq Size	6320 bp
RefSeq ORF	1344 bp
Locus ID	9066
UniProt ID	O43581
Protein Families	Secreted Protein, Transmembrane
MW	51.2 kDa
Gene Summary	This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. A similar protein in rodents mediates hormone secretion and lysosome exocytosis. In humans, expression of this gene has been associated with prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

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