CDC2L6 (CDK19) (NM_001300963) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bA346C16.3; CDC2L6; CDK11; DEE87; EIEE87
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238257 representing NM_001300963 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCTTGTAGAGAGATTGCACTTTTGCGAGAATTGAAGCACCCTAATGTGATTGCATTGCAGAAGG TGTTCCTTTCTCACAGTGACAGGAAGGTATGGCTGCTGTTTGATTATGCAGAGCATGACTTGTGGCATAT TATTAAGTTTCACCGTGCATCAAAAGCAAATAAAAAGCCCATGCAGTTGCCAAGATCTATGGTTAAATCC TTACTTTACCAGATTCTTGATGGTATCCATTACCTCCATGCAAATTGGGTGCTTCACAGAGACTTGAAAC CAGCAAATATCCTAGTAATGGGAGAAGGTCCTGAGAGGGGGAGAGTCAAAATAGCTGACATGGGTTTTGC CAGATTATTCAATTCTCCTCTAAAGCCACTAGCAGATTTGGATCCAGTAGTTGTGACATTTTGGTATCGG GCTCCAGAACTTTTGCTTGGTGCAAGGCATTATACAAAGGCCATTGATATATGGGCAATAGGTTGTATAT TTGCTGAATTGTTGACTTCGGAACCTATTTTTCACTGTCGTCAGGAAGATATAAAAACAAGCAATCCCTT TCATCATGATCAACTGGATCGGATATTTAGTGTCATGGGGTTTCCTGCAGATAAAGACTGGGAAGATATT AGAAAGATGCCAGAATATCCCACACTTCAAAAAGACTTTAGAAGAACAACGTATGCCAACAGTAGCCTCA TAAAGTACATGGAGAAACACAAGGTCAAGCCTGACAGCAAAGTGTTCCTCTTGCTTCAGAAACTCCTGAC CATGGATCCAACCAAGAGAATTACCTCGGAGCAAGCTCTGCAGGATCCCTATTTTCAGGAGGACCCTTTG CCAACATTAGATGTATTTGCCGGCTGCCAGATTCCATACCCCAAACGAGAATTCCTTAATGAAGATGATC CTGAAGAAAAAGGTGACAAGAATCAGCAACAGCAGCAGAACCAGCATCAGCAGCCCACAGCCCCTCCACA GCAGGCAGCAGCCCCTCCACAGGCGCCCCCACCACAGCAGAACAGCACCCAGACCAACGGGACCGCAGGT GGGGCTGGGGCCGGGGTCGGGGGCACCGGAGCAGGGTTGCAGCACAGCCAGGACTCCAGCCTGAACCAGG TGCCTCCAAACAAGAAGCCACGGCTAGGGCCTTCAGGCGCAAACTCAGGTGGACCTGTGATGCCCTCGGA TTATCAGCACTCCAGTTCTCGCCTGAATTACCAAAGCAGCGTTCAGGGATCCTCTCAGTCCCAGAGCACA CTTGGCTACTCTTCCTCGTCTCAGCAGAGCTCACAGTACCACCCATCTCACCAGGCCCACCGGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238257 representing NM_001300963 Red=Cloning site Green=Tags(s) MSACREIALLRELKHPNVIALQKVFLSHSDRKVWLLFDYAEHDLWHIIKFHRASKANKKPMQLPRSMVKS LLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYR APELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPFHHDQLDRIFSVMGFPADKDWEDI RKMPEYPTLQKDFRRTTYANSSLIKYMEKHKVKPDSKVFLLLQKLLTMDPTKRITSEQALQDPYFQEDPL PTLDVFAGCQIPYPKREFLNEDDPEEKGDKNQQQQQNQHQQPTAPPQQAAAPPQAPPPQQNSTQTNGTAG GAGAGVGGTGAGLQHSQDSSLNQVPPNKKPRLGPSGANSGGPVMPSDYQHSSSRLNYQSSVQGSSQSQST LGYSSSSQQSSQYHPSHQAHRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300963
ORF Size	1326 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300963.1, NP_001287892.1
RefSeq Size	6062 bp
RefSeq ORF	1329 bp
Locus ID	23097
UniProt ID	Q9BWU1
Protein Families	Druggable Genome, Protein Kinase
MW	50.3 kDa
Gene Summary	This gene encodes a protein that is one of the components of the Mediator co-activator complex. The Mediator complex is a multi-protein complex required for transcriptional activation by DNA binding transcription factors of genes transcribed by RNA polymerase II. The protein encoded by this gene is similar to cyclin-dependent kinase 8 which can also be a component of the Mediator complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]

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