Transmembrane Protein 175 (TMEM175) (NM_001297427) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	hTMEM175
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237907 representing NM_001297427 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGACCATCACCTTCCTGCCTTACACGTTTTCGTTAATGGTGACCTTCCCTGATGTGCCTCTGGGCA TCTTCTTGTTCTGTGTGTGTGTGATCGCCATTGGGGTCGTGCAGGCACTGATTGTGGGGTACGCATTCCA CTTCCCGCACCTGCTGAGCCCGCAGATCCAGCGCTCTGCCCACAGGGCTCTGTACCGACGACACGTCCTG GGCATCGTCCTCCAAGGCCCGGCCCTGTGCTTTGCAGCGGCCATCTTCTCTCTCTTCTTTGTCCCCTTGT CTTACCTGCTGATGGTGACTGTCATCCTCCTCCCCTATGTCAGCAAGGTCACCGGCTGGTGCAGAGACAG GCTCCTGGGCCACAGGGAGCCCTCGGCTCACCCAGTGGAAGTCTTCTCGTTTGACCTCCACGAGCCACTC AGCAAGGAGCGCGTGGAAGCCTTCAGCGACGGAGTCTACGCCATCGTGGCCACGCTTCTCATCCTGGACA TCTGCGAAGACAACGTCCCGGACCCCAAGGATGTGAAGGAGAGGTTCAGCGGCAGCCTCGTGGCCGCCCT GAGTGCGACCGGGCCGCGCTTCCTGGCGTACTTCGGCTCCTTCGCCACAGTGGGACTGCTGTGGTTCGCC CACCACTCACTCTTCCTGCATGTGCGCAAGGCCACGCGGGCCATGGGGCTGCTGAACACGCTCTCGCTGG CCTTCGTGGGTGGCCTCCCACTAGCCTACCAGCAGACCTCGGCCTTCGCCCGGCAGCCCCGCGATGAGCT GGAGCGCGTGCGTGTCAGCTGCACCATCATCTTCCTGGCCAGCATCTTCCAGCTGGCCATGTGGACCACG GCGCTGCTGCACCAGGCGGAGACGCTGCAGCCCTCGGTGTGGTTTGGCGGCCGGGAGCATGTGCTCATGT TCGCCAAGCTGGCGCTGTACCCCTGTGCCAGCCTGCTGGCCTTCGCCTCCACCTGCCTGCTGAGCAGGTT CAGTGTGGGCATCTTCCACCTCATGCAGATCGCCGTGCCCTGCGCCTTCCTGTTGCTGCGCCTGCTCGTG GGCCTGGCCCTGGCCACCCTGCGGGTCCTGCGGGGCCTCGCCCGGCCCGAACACCCCCCGCCAGCCCCCA CGGGCCAGGACGACCCACAGTCCCAGCTCCTCCCTGCCCCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237907 representing NM_001297427 Red=Cloning site Green=Tags(s) MMTITFLPYTFSLMVTFPDVPLGIFLFCVCVIAIGVVQALIVGYAFHFPHLLSPQIQRSAHRALYRRHVL GIVLQGPALCFAAAIFSLFFVPLSYLLMVTVILLPYVSKVTGWCRDRLLGHREPSAHPVEVFSFDLHEPL SKERVEAFSDGVYAIVATLLILDICEDNVPDPKDVKERFSGSLVAALSATGPRFLAYFGSFATVGLLWFA HHSLFLHVRKATRAMGLLNTLSLAFVGGLPLAYQQTSAFARQPRDELERVRVSCTIIFLASIFQLAMWTT ALLHQAETLQPSVWFGGREHVLMFAKLALYPCASLLAFASTCLLSRFSVGIFHLMQIAVPCAFLLLRLLV GLALATLRVLRGLARPEHPPPAPTGQDDPQSQLLPAPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297427
ORF Size	1164 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297427.2
RefSeq Size	2381 bp
RefSeq ORF	1167 bp
Locus ID	84286
UniProt ID	Q9BSA9
Protein Families	Transmembrane
MW	43.3 kDa
Gene Summary	Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.