PFTK1 (CDK14) (NM_001287137) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PFTAIRE1; PFTK1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237576 representing NM_001287137 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGCATTAACTTTAAGACCTCCTCCACTGGCAAAGAGTCACCTAAAGTTAGGCGGCACTCCAGCCC CAGCTCGGGTAAATGGGAAGTTGGTAGCTCTGAAGGTGATCAGGCTGCAGGAAGAAGAAGGGACACCTTT CACAGCTATCAGGGAAGCTTCTCTTTTAAAAGGACTAAAACATGCTAACATAGTGCTACTTCATGACATC ATCCATACCAAGGAGACGCTGACACTTGTGTTTGAATATGTGCACACTGATTTATGTCAGTACATGGACA AGCACCCTGGGGGGCTGCATCCAGATAATGTGAAGTTGTTTTTATTTCAGTTGCTGCGAGGTCTGTCTTA CATCCACCAGCGTTATATTTTGCACAGAGACCTGAAACCACAGAACCTTCTGATCAGTGACACGGGGGAG TTAAAGCTGGCAGATTTCGGTCTTGCAAGAGCAAAATCCGTCCCTAGCCACACATACTCCAACGAAGTGG TTACCTTGTGGTACAGACCTCCAGATGTCCTTCTAGGCTCAACAGAATATTCCACCTGCCTTGACATGTG GGGAGTAGGTTGCATCTTTGTTGAAATGATCCAAGGAGTTGCTGCTTTTCCAGGAATGAAAGACATTCAG GATCAACTTGAACGAATATTTCTGGTTCTTGGAACACCAAATGAGGACACATGGCCTGGAGTTCATTCTT TACCACATTTTAAGCCAGAACGCTTTACCCTGTACAGCTCTAAAAACCTTAGACAAGCATGGAATAAGCT CAGCTATGTGAACCATGCAGAGGACCTGGCCTCCAAGCTCCTACAATGTTCCCCAAAGAACAGACTGTCG GCACAGGCTGCCTTGAGCCACGAGTATTTTAGTGACCTGCCGCCACGGCTATGGGAACTCACCGACATGT CTTCTATTTTTACTGTCCCAAATGTGAGATTGCAACCAGAAGCTGGAGAAAGCATGCGGGCCTTTGGGAA AAACAATAGTTATGGCAAAAGTCTATCAAACAGCAAGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237576 representing NM_001287137 Red=Cloning site Green=Tags(s) MLALTLRPPPLAKSHLKLGGTPAPARVNGKLVALKVIRLQEEEGTPFTAIREASLLKGLKHANIVLLHDI IHTKETLTLVFEYVHTDLCQYMDKHPGGLHPDNVKLFLFQLLRGLSYIHQRYILHRDLKPQNLLISDTGE LKLADFGLARAKSVPSHTYSNEVVTLWYRPPDVLLGSTEYSTCLDMWGVGCIFVEMIQGVAAFPGMKDIQ DQLERIFLVLGTPNEDTWPGVHSLPHFKPERFTLYSSKNLRQAWNKLSYVNHAEDLASKLLQCSPKNRLS AQAALSHEYFSDLPPRLWELTDMSSIFTVPNVRLQPEAGESMRAFGKNNSYGKSLSNSKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287137
ORF Size	1020 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287137.1, NP_001274066.1
RefSeq Size	4804 bp
RefSeq ORF	1023 bp
Locus ID	5218
UniProt ID	O94921
Protein Families	Druggable Genome, Protein Kinase
MW	38.8 kDa
Gene Summary	PFTK1 is a member of the CDC2 (MIM 116940)-related protein kinase family (Yang and Chen, 2001 [PubMed 11313143]).[supplied by OMIM, Mar 2008]

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