MAEA (NM_001297430) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EMLP; EMP; GID9; HLC-10; P44EMLP; PIG5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237473 representing NM_001297430 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGCAGGAGTCGGCGGCTCAGTTGTCCATGACCCTGAAGGTCCAGGAGTACCCGACCCTCAAGG TGCCCTACGAGACGCTGAACAAACGCTTTCGCGCCGCTCAGAAGAACATTGACCGGGAGACCAGCCACGT CACCATGGTGGTGGCCGAGCTGGAGAAGACGTTGAGCGGCTGCCCCGCCGTGGACTCCGTGGTCAGCCTG CTGGACGGCGTGGTGGAGAAGCTCAGCGTCCTCAAGAGGAAGGACCTAGTGAATATTGAGATGTTCCTGA CGGCCAAAGAGGTGGAGGAGTCCCTGGAGAGGCGTGAGACGGCCACCTGCCTGGCCTGGTGCCATGACAA CAAGTCCCGGCTCCGGAAGATGAAGAGCTGCCTGGAGTTCAGCCTCAGAATCCAGGAGTTCATTGAACTC ATCCGGCAGAATAAGAGACTGGACGCTGTGAGACATGCAAGAAAGCACTTCAGCCAAGCAGAAGGGAGCC AGCTGGACGAGGTGCGCCAGGCCATGGGCATGCTGGCCTTCCCGCCCGACACGCACATCTCCCCGTACAA GGACCTTCTGGACCCTGCACGGTGGCGGATGCTGATCCAGCAGTTCCGGTACGACAACTACCGACTACAC CAGCTGGGAAACAATTCTGTGTTCACCCTCACCCTGCAGGCTGGCCTCTCAGCCATCAAGACACCACAGT GCTACAAGGAGGACGGCAGCTCCAAGAGCCCTGACTGCCCTGTGTGCAGCCGCTCCCTGAACAAGCTGGC GCAGCCCCTGCCCATGGCCCACTGTGCCAACTCCCGCCTGGTCTGCAAGATTTCTGGCGACGTGATGAAC GAGAACAATCCGCCCATGATGCTGCCCAACGGCTACGTCTACGGCTACAATTCTCTGCTTTCTATCCGTC AAGATGATAAAGTCGTGTGCCCGAGAACCAAAGAAGTCTTCCACTTCTCACAAGCCGAGAAGGTGTACAT CATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237473 representing NM_001297430 Red=Cloning site Green=Tags(s) MAVQESAAQLSMTLKVQEYPTLKVPYETLNKRFRAAQKNIDRETSHVTMVVAELEKTLSGCPAVDSVVSL LDGVVEKLSVLKRKDLVNIEMFLTAKEVEESLERRETATCLAWCHDNKSRLRKMKSCLEFSLRIQEFIEL IRQNKRLDAVRHARKHFSQAEGSQLDEVRQAMGMLAFPPDTHISPYKDLLDPARWRMLIQQFRYDNYRLH QLGNNSVFTLTLQAGLSAIKTPQCYKEDGSSKSPDCPVCSRSLNKLAQPLPMAHCANSRLVCKISGDVMN ENNPPMMLPNGYVYGYNSLLSIRQDDKVVCPRTKEVFHFSQAEKVYIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297430
ORF Size	984 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297430.2
RefSeq Size	2046 bp
RefSeq ORF	987 bp
Locus ID	10296
Protein Families	Druggable Genome
MW	37.9 kDa
Gene Summary	This gene encodes a protein that mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. The encoded protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

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