MYBPC1 (NM_001254723) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	LCCS4; MYBPCC; MYBPCS; MYOTREM; ssMyBP-C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235183 representing NM_001254723 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGAACCCACTAAGAAAGAGGATGAAGAGGAAGTCTCCCCGCCTAGCGCCTTGCCTCCAGACTGGA CCCTTGTCGAAACTCCTCCTGGGGAGGAACAAGCCAAGCAGAATGCCAACTCCCAGCTGTCCATCTTGTT CATTGAAAAACCTCAAGGAGGAACAGTGAAAGTTGGTGAAGATATCACCTTCATAGCCAAAGTCAAGGCT GAAGATCTTCTGAGAAAACCCACTATCAAATGGTTCAAAGGAAAATGGATGGACCTGGCCAGCAAAGCCG GGAAGCACCTTCAGCTGAAGGAAACCTTTGAGAGGCACAGTCGGGTGTACACATTTGAGATGCAGATCAT CAAGGCCAAAGATAACTTTGCAGGAAATTACAGATGCGAGGTCACCTATAAGGATAAGTTTGACAGCTGT TCATTTGATCTTGAAGTGCACGAATCTACTGGGACTACTCCAAACATTGACATCAGATCTGCTTTCAAGA GAAGTGGAGAAGGTCAAGAGGATGCAGGAGAACTTGACTTTAGTGGTCTCCTGAAACGTAGGGAGGTGAA GCAGCAGGAGGAAGAACCCCAGGTGGACGTATGGGAGTTGCTGAAGAACGCGAAACCCAGTGAGTACGAG AAGATCGCCTTCCAGTATGGAATCACCGACCTGCGCGGCATGCTCAAGCGACTCAAGCGCATGCGCAGAG AGGAGAAGAAGAGCGCAGCTTTTGCAAAAATTCTTGATCCTGCATATCAGGTTGACAAAGGAGGCAGAGT GAGGTTTGTTGTGGAGCTGGCAGATCCAAAGTTGGAGGTGAAATGGTATAAAAATGGTCAAGAAATTCGA CCCAGTACCAAATACATCTTTGAACACAAAGGATGCCAGAGAATCCTGTTTATCAATAACTGTCAGATGA CAGATGATTCAGAGTATTATGTGACAGCCGGTGATGAGAAATGTTCCACTGAGCTCTTCGTAAGAGAGCC TCCAATTATGGTGACCAAACAGCTGGAAGATACAACTGCTTATTGTGGGGAGAGAGTGGAATTAGAATGT GAGGTGTCTGAAGATGATGCCAATGTAAAATGGTTTAAGAATGGTGAAGAGATTATCCCTGGTCCAAAAT CAAGATACCGAATTAGAGTTGAGGGTAAAAAACACATCTTGATCATAGAGGGAGCAACAAAGGCTGATGC TGCAGAATATTCAGTAATGACAACAGGAGGACAATCATCTGCTAAACTTAGTGTTGACTTGAAACCTCTG AAGATTTTGACACCTCTGACTGATCAGACTGTAAATCTTGGAAAAGAAATCTGCCTGAAGTGTGAAATCT CTGAAAACATACCAGGAAAATGGACTAAAAATGGCCTACCTGTTCAGGAGAGTGACCGTCTAAAGGTGGT TCACAAGGGAAGGATCCACAAGTTAGTGATAGCCAATGCCCTCACTGAAGATGAAGGTGATTATGTATTT GCACCTGATGCCTACAATGTTACTCTGCCTGCCAAAGTTCATGTTATTGATCCTCCTAAGATCATCCTGG ATGGTCTTGATGCTGACAACACAGTGACAGTGATTGCAGGAAACAAGCTTCGTCTTGAGATCCCCATCAG CGGAGAACCACCTCCTAAAGCCATGTGGAGCCGGGGAGATAAGGCTATTATGGAAGGCAGTGGCCGGATA AGAACAGAATCTTACCCTGATAGCAGCACTCTGGTCATTGATATAGCTGAAAGAGATGACTCTGGTGTTT ACCACATCAATCTGAAAAACGAAGCTGGAGAGGCACATGCAAGCATCAAGGTTAAAGTTGTGGACTTCCC TGATCCTCCAGTGGCACCGACTGTGACAGAGGTGGGAGATGACTGGTGTATCATGAACTGGGAGCCTCCT GCCTACGACGGAGGCTCTCCAATCCTAGGATATTTTATTGAGAGGAAGAAGAAACAAAGCTCCAGGTGGA TGAGGCTGAATTTTGATCTCTGCAAAGAAACAACTTTTGAGCCCAAGAAGATGATTGAAGGTGTGGCCTA TGAGGTCCGCATCTTTGCAGTCAATGCCATTGGCATCTCCAAGCCCAGTATGCCCTCCAGGCCTTTTGTT CCTTTGGCTGTAACAAGCCCTCCTACTCTTCTGACTGTGGACTCTGTCACTGACACGACTGTCACGATGA GGTGGCGCCCCCCAGACCACATTGGTGCAGCAGGTTTAGATGGCTATGTGCTAGAGTATTGCTTTGAAGG AACTGAGGACTGGATAGTTGCAAACAAAGATCTGATTGACAAGACGAAGTTCACCATCACAGGTCTGCCA ACAGATGCAAAGATCTTTGTGCGTGTGAAGGCTGTTAATGCAGCTGGTGCCAGCGAGCCCAAGTACTATT CTCAGCCCATTCTCGTGAAGGAAATCATAGAACCTCCAAAGATTCGCATTCCAAGACACCTGAAGCAAAC CTATATCCGCAGAGTTGGAGAAGCTGTCAATCTGGTTATACCTTTCCAGGGAAAACCAAGACCAGAATTA ACTTGGAAGAAGGATGGTGCAGAAATTGATAAGAATCAAATAAACATTCGCAACTCTGAGACTGATACAA TCATATTTATTAGAAAAGCAGAGAGGAGCCACTCTGGGAAATATGATCTGCAAGTCAAAGTGGACAAATT CGTGGAGACCGCATCAATTGACATCCAGATCATTGACCGTCCAGGTCCACCCCAAATTGTGAAGATTGAG GATGTCTGGGGAGAAAATGTCGCTCTCACATGGACTCCACCAAAGGATGATGGAAATGCTGCTATCACAG GCTATACCATTCAGAAGGCTGACAAGAAGAGCATGGAATGGTTTACTGTCATTGAGCATTATCATCGAAC CAGTGCCACCATTACTGAATTGGTCATAGGGAATGAATATTACTTCCGGGTCTTTTCTGAAAACATGTGT GGCCTCAGTGAGGATGCCACCATGACTAAAGAGAGTGCAGTGATCGCCAGGGATGGTAAAATCTACAAAA ATCCAGTGTATGAAGACTTTGATTTCTCAGAGGCACCCATGTTTACTCAGCCTTTGGTTAACACCTATGC CATAGCTGGTTACAATGCCACCCTAAACTGCAGTGTGAGAGGAAATCCTAAGCCTAAAATAACCTGGATG AAAAACAAAGTTGCTATTGTGGATGATCCAAGATACAGGATGTTCAGCAACCAGGGAGTCTGTACCCTGG AAATTCGCAAGCCCAGCCCCTATGATGGAGGCACTTACTGCTGCAAAGCAGTCAATGACCTTGGGACAGT GGAGATTGAATGCAAACTGGAGGTGAAAGGTGGGCTCTCCTTCTGCAGACTCCTCTTGCAAGGCGTACCT CCAAACATAATTGATTCGTATCTGCGAGACTTACACTCAAGCAATCCTGAGGAATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235183 representing NM_001254723 Red=Cloning site Green=Tags(s) MPEPTKKEDEEEVSPPSALPPDWTLVETPPGEEQAKQNANSQLSILFIEKPQGGTVKVGEDITFIAKVKA EDLLRKPTIKWFKGKWMDLASKAGKHLQLKETFERHSRVYTFEMQIIKAKDNFAGNYRCEVTYKDKFDSC SFDLEVHESTGTTPNIDIRSAFKRSGEGQEDAGELDFSGLLKRREVKQQEEEPQVDVWELLKNAKPSEYE KIAFQYGITDLRGMLKRLKRMRREEKKSAAFAKILDPAYQVDKGGRVRFVVELADPKLEVKWYKNGQEIR PSTKYIFEHKGCQRILFINNCQMTDDSEYYVTAGDEKCSTELFVREPPIMVTKQLEDTTAYCGERVELEC EVSEDDANVKWFKNGEEIIPGPKSRYRIRVEGKKHILIIEGATKADAAEYSVMTTGGQSSAKLSVDLKPL KILTPLTDQTVNLGKEICLKCEISENIPGKWTKNGLPVQESDRLKVVHKGRIHKLVIANALTEDEGDYVF APDAYNVTLPAKVHVIDPPKIILDGLDADNTVTVIAGNKLRLEIPISGEPPPKAMWSRGDKAIMEGSGRI RTESYPDSSTLVIDIAERDDSGVYHINLKNEAGEAHASIKVKVVDFPDPPVAPTVTEVGDDWCIMNWEPP AYDGGSPILGYFIERKKKQSSRWMRLNFDLCKETTFEPKKMIEGVAYEVRIFAVNAIGISKPSMPSRPFV PLAVTSPPTLLTVDSVTDTTVTMRWRPPDHIGAAGLDGYVLEYCFEGTEDWIVANKDLIDKTKFTITGLP TDAKIFVRVKAVNAAGASEPKYYSQPILVKEIIEPPKIRIPRHLKQTYIRRVGEAVNLVIPFQGKPRPEL TWKKDGAEIDKNQINIRNSETDTIIFIRKAERSHSGKYDLQVKVDKFVETASIDIQIIDRPGPPQIVKIE DVWGENVALTWTPPKDDGNAAITGYTIQKADKKSMEWFTVIEHYHRTSATITELVIGNEYYFRVFSENMC GLSEDATMTKESAVIARDGKIYKNPVYEDFDFSEAPMFTQPLVNTYAIAGYNATLNCSVRGNPKPKITWM KNKVAIVDDPRYRMFSNQGVCTLEIRKPSPYDGGTYCCKAVNDLGTVEIECKLEVKGGLSFCRLLLQGVP PNIIDSYLRDLHSSNPEEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001254723
ORF Size	3417 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001254723.1, NP_001241652.1
RefSeq Size	3778 bp
RefSeq ORF	3420 bp
Locus ID	4604
UniProt ID	Q00872
MW	128.8 kDa
Gene Summary	This gene encodes a member of the myosin-binding protein C family. Myosin-binding protein C family members are myosin-associated proteins found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The encoded protein is the slow skeletal muscle isoform of myosin-binding protein C and plays an important role in muscle contraction by recruiting muscle-type creatine kinase to myosin filaments. Mutations in this gene are associated with distal arthrogryposis type I. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

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