NFAT1 (NFATC2) (NM_001258296) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NFAT1; NFATP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234746 representing NM_001258296 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACCTCGAACCAGCCTCGCCGAGGACAGCTGCCTGGGCCGCCACTCGCCCGTGCCCCGTCCGGCCT CCCGCTCCTCATCGCCTGGTGCCAAGCGGAGGCATTCGTGCGCCGAGGCCTTGGTTGCCCTGCCGCCCGG AGCCTCACCCCAGCGCTCCCGGAGCCCCTCGCCGCAGCCCTCATCTCACGTGGCACCCCAGGACCACGGC TCCCCGGCTGGGTACCCCCCTGTGGCTGGCTCTGCCGTGATCATGGATGCCCTGAACAGCCTCGCCACGG ACTCGCCTTGTGGGATCCCCCCCAAGATGTGGAAGACCAGCCCTGACCCCTCGCCGGTGTCTGCCGCCCC ATCCAAGGCCGGCCTGCCTCGCCACATCTACCCGGCCGTGGAGTTCCTGGGGCCCTGCGAGCAGGGCGAG AGGAGAAACTCGGCTCCAGAATCCATCCTGCTGGTTCCGCCCACTTGGCCCAAGCCGCTGGTGCCTGCCA TTCCCATCTGCAGCATCCCAGTGACTGCATCCCTCCCTCCACTTGAGTGGCCGCTGTCCAGTCAGTCAGG CTCTTACGAGCTGCGGATCGAGGTGCAGCCCAAGCCACATCACCGGGCCCACTATGAGACAGAAGGCAGC CGAGGGGCTGTCAAAGCTCCAACTGGAGGCCACCCTGTGGTTCAGCTCCATGGCTACATGGAAAACAAGC CTCTGGGACTTCAGATCTTCATTGGGACAGCTGATGAGCGGATCCTTAAGCCGCACGCCTTCTACCAGGT GCACCGAATCACGGGGAAAACTGTCACCACCACCAGCTATGAGAAGATAGTGGGCAACACCAAAGTCCTG GAGATACCCTTGGAGCCCAAAAACAACATGAGGGCAACCATCGACTGTGCGGGGATCTTGAAGCTTAGAA ACGCCGACATTGAGCTGCGGAAAGGCGAGACGGACATTGGAAGAAAGAACACGCGGGTGAGACTGGTTTT CCGAGTTCACATCCCAGAGTCCAGTGGCAGAATCGTCTCTTTACAGACTGCATCTAACCCCATCGAGTGC TCCCAGCGATCTGCTCACGAGCTGCCCATGGTTGAAAGACAAGACACAGACAGCTGCCTGGTCTATGGCG GCCAGCAAATGATCCTCACGGGGCAGAACTTTACATCCGAGTCCAAAGTTGTGTTTACTGAGAAGACCAC AGATGGACAGCAAATTTGGGAGATGGAAGCCACGGTGGATAAGGACAAGAGCCAGCCCAACATGCTTTTT GTTGAGATCCCTGAATATCGGAACAAGCATATCCGCACACCTGTAAAAGTGAACTTCTACGTCATCAATG GGAAGAGAAAACGAAGTCAGCCTCAGCACTTTACCTACCACCCAGTCCCAGCCATCAAGACGGAGCCCAC GGATGAATATGACCCCACTCTGATCTGCAGCCCCACCCATGGAGGCCTGGGGAGCCAGCCTTACTACCCC CAGCACCCGATGGTGGCCGAGTCCCCCTCCTGCCTCGTGGCCACCATGGCTCCCTGCCAGCAGTTCCGCA CGGGGCTCTCATCCCCTGACGCCCGCTACCAGCAACAGAACCCAGCGGCCGTACTCTACCAGCGGAGCAA GAGCCTGAGCCCCAGCCTGCTGGGCTATCAGCAGCCGGCCCTCATGGCCGCCCCGCTGTCCCTTGCGGAC GCTCACCGCTCTGTGCTGGTGCACGCCGGCTCCCAGGGCCAGAGCTCAGCCCTGCTCCACCCCTCTCCGA CCAACCAGCAGGCCTCGCCTGTGATCCACTACTCACCCACCAACCAGCAGCTGCGCTGCGGAAGCCACCA GGAGTTCCAGCACATCATGTACTGCGAGAATTTCGCACCAGGCACCACCAGACCTGGCCCGCCCCCGGTC AGTCAAGGTCAGAGGCTGAGCCCGGGTTCCTACCCCACAGTCATTCAGCAGCAGAATGCCACGAGCCAAA GAGCCGCCAAAAACGGACCCCCGGTCAGTGACCAAAAGGAAGTATTACCTGCGGGGGTGACCATTAAACA GGAGCAGAACTTGGACCAGACCTACTTGGATGATGAGCTGATAGACACACACCTTAGCTGGATACAAAAC ATATTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234746 representing NM_001258296 Red=Cloning site Green=Tags(s) MSPRTSLAEDSCLGRHSPVPRPASRSSSPGAKRRHSCAEALVALPPGASPQRSRSPSPQPSSHVAPQDHG SPAGYPPVAGSAVIMDALNSLATDSPCGIPPKMWKTSPDPSPVSAAPSKAGLPRHIYPAVEFLGPCEQGE RRNSAPESILLVPPTWPKPLVPAIPICSIPVTASLPPLEWPLSSQSGSYELRIEVQPKPHHRAHYETEGS RGAVKAPTGGHPVVQLHGYMENKPLGLQIFIGTADERILKPHAFYQVHRITGKTVTTTSYEKIVGNTKVL EIPLEPKNNMRATIDCAGILKLRNADIELRKGETDIGRKNTRVRLVFRVHIPESSGRIVSLQTASNPIEC SQRSAHELPMVERQDTDSCLVYGGQQMILTGQNFTSESKVVFTEKTTDGQQIWEMEATVDKDKSQPNMLF VEIPEYRNKHIRTPVKVNFYVINGKRKRSQPQHFTYHPVPAIKTEPTDEYDPTLICSPTHGGLGSQPYYP QHPMVAESPSCLVATMAPCQQFRTGLSSPDARYQQQNPAAVLYQRSKSLSPSLLGYQQPALMAAPLSLAD AHRSVLVHAGSQGQSSALLHPSPTNQQASPVIHYSPTNQQLRCGSHQEFQHIMYCENFAPGTTRPGPPPV SQGQRLSPGSYPTVIQQQNATSQRAAKNGPPVSDQKEVLPAGVTIKQEQNLDQTYLDDELIDTHLSWIQN IL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258296
ORF Size	2106 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258296.2
RefSeq Size	7016 bp
RefSeq ORF	2109 bp
Locus ID	4773
UniProt ID	Q13469
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
MW	77.1 kDa
Gene Summary	This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]

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