LATS1 (NM_001270519) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	WARTS; wts
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234723 representing NM_001270519 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAGGAGTGAAAAGCCAGAAGGATATAGACAAATGAGGCCTAAGACCTTTCCTGCCAGTAACTATA CTGTCAGTAGCCGGCAAATGTTACAAGAAATTCGGGAATCCCTTAGGAATTTATCTAAACCATCTGATGC TGCTAAGGCTGAGCATAACATGAGTAAAATGTCAACCGAAGATCCTCGACAAGTCAGAAATCCACCCAAA TTTGGGACGCATCATAAAGCCTTGCAGGAAATTCGAAACTCTCTGCTTCCATTTGCAAATGAAACAAATT CTTCTCGGAGTACTTCAGAAGTTAATCCACAAATGCTTCAAGACTTGCAAGCTGCTGGATTTGATGAGGA TATGGTTATACAAGCTCTTCAGAAAACTAACAACAGAAGTATAGAAGCAGCAATTGAATTCATTAGTAAA ATGAGTTACCAAGATCCTCGACGAGAGCAGATGGCTGCAGCAGCTGCCAGACCTATTAATGCCAGCATGA AACCAGGGAATGTGCAGCAATCAGTTAACCGCAAACAGAGCTGGAAAGGTTCTAAAGAATCCTTAGTTCC TCAGAGGCATGGCCCGCCACTAGGAGAAAGTGTGGCCTATCATTCTGAGAGTCCCAACTCACAGACAGAT GTAGGAAGACCTTTGTCTGGATCTGGTATATCAGCATTTGTTCAAGCTCACCCTAGCAACGGACAGAGAG TGAACCCCCCACCACCACCTCAAGTAAGGAGTGTTACTCCTCCACCACCTCCAAGAGGCCAGACTCCCCC TCCAAGAGGTACAACTCCACCTCCCCCTTCATGGGAACCAAACTCTCAAACAAAGCGCTATTCTGGAAAC ATGGAATACGTAATCTCCCGAATCTCTCCTGTCCCACCTGGGGCATGGCAAGAGGGCTATCCTCCACCAC CTCTCAACACTTCCCCCATGAATCCTCCTAATCAAGGACAGAGAGGCATTAGTTCTGTTCCTGTTGGCAG ACAACCAATCATCATGCAGAGTTCTAGCAAATTTAACTTTCCATCAGGGAGACCTGGAATGCAGAATGGT ACTGGACAAACTGATTTCATGATACACCAAAATGTTGTCCCTGCTGGCACTGTGAATCGGCAGCCACCAC CTCCATATCCTCTGACAGCAGCTAATGGACAAAGCCCTTCTGCTTTACAAACAGGGGGATCTGCTGCTCC TTCGTCATATACAAATGGAAGTATTCCTCAGTCTATGATGGTGCCAAACAGAAATAGTCATAACATGGAA CTATATAACATTAGTGTACCTGGACTGCAAACAAATTGGCCTCAGTCATCTTCTGCTCCAGCCCAGTCAT CCCCGAGCAGTGGGCATGAAATCCCTACATGGCAACCTAACATACCAGTGAGGTCAAATTCTTTTAATAA CCCATTAGGAAATAGAGCAAGTCACTCTGCTAATTCTCAGCCTTCTGCTACAACAGTCACTGCAATTACA CCAGCTCCTATTCAACAGCCTGTGAAAAGTATGCGTGTATTAAAACCAGAGCTACAGACTGCTTTAGCAC CTACACACCCTTCTTGGATACCACAGCCAATTCAAACTGTTCAACCCAGTCCTTTTCCTGAGGGAACCGC TTCAAATGTGACTGTGATGCCACCTGTTGCTGAAGCTCCAAACTATCAAGGACCACCACCACCCTACCCA AAACATCTGCTGCACCAAAACCCATCTGTTCCTCCATACGAGTCAATCAGTAAGCCTAGCAAAGAGGATC AGCCAAGCTTGCCCAAGGAAGATGAGAGTGAAAAGAGTTATGAAAATGTTGATAGTGGGGATAAAGAAAA GAAACAGATTACAACTTCACCTATTACTGTTAGGAAAAACAAGAAAGATGAAGAGCGAAGGGAATCTCGT ATTCAAAGTTATTCTCCTCAAGCATTTAAATTCTTTATGGAGCAACATGTAGAAAATGTACTCAAATCTC ATCAGCAGCGTCTACATCGTAAAAAACAATTAGAGAATGAAATGATGCGGGTAAAACCTTTTAAAATGTC CATTTTTATACTTAATCATCTGTTTGCTTGGTGTTTATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234723 representing NM_001270519 Red=Cloning site Green=Tags(s) MKRSEKPEGYRQMRPKTFPASNYTVSSRQMLQEIRESLRNLSKPSDAAKAEHNMSKMSTEDPRQVRNPPK FGTHHKALQEIRNSLLPFANETNSSRSTSEVNPQMLQDLQAAGFDEDMVIQALQKTNNRSIEAAIEFISK MSYQDPRREQMAAAAARPINASMKPGNVQQSVNRKQSWKGSKESLVPQRHGPPLGESVAYHSESPNSQTD VGRPLSGSGISAFVQAHPSNGQRVNPPPPPQVRSVTPPPPPRGQTPPPRGTTPPPPSWEPNSQTKRYSGN MEYVISRISPVPPGAWQEGYPPPPLNTSPMNPPNQGQRGISSVPVGRQPIIMQSSSKFNFPSGRPGMQNG TGQTDFMIHQNVVPAGTVNRQPPPPYPLTAANGQSPSALQTGGSAAPSSYTNGSIPQSMMVPNRNSHNME LYNISVPGLQTNWPQSSSAPAQSSPSSGHEIPTWQPNIPVRSNSFNNPLGNRASHSANSQPSATTVTAIT PAPIQQPVKSMRVLKPELQTALAPTHPSWIPQPIQTVQPSPFPEGTASNVTVMPPVAEAPNYQGPPPPYP KHLLHQNPSVPPYESISKPSKEDQPSLPKEDESEKSYENVDSGDKEKKQITTSPITVRKNKKDEERRESR IQSYSPQAFKFFMEQHVENVLKSHQQRLHRKKQLENEMMRVKPFKMSIFILNHLFAWCLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270519
ORF Size	2070 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270519.1, NP_001257448.1
RefSeq Size	2743 bp
RefSeq ORF	2073 bp
Locus ID	9113
UniProt ID	O95835
Protein Families	Druggable Genome, Protein Kinase
MW	76.6 kDa
Gene Summary	The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq, Apr 2017]

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