PRRT2 (NM_001256443) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BFIC2; BFIS2; DSPB3; DYT10; EKD1; FICCA; ICCA; IFITMD1; PKC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233691 representing NM_001256443 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCAGCAGCTCTGAGATCTCTGAGATGAAGGGGGTTGAGGAGAGTCCCAAGGTTCCAGGCGAAG GGCCTGGCCATTCTGAAGCTGAAACTGGCCCTCCCCAGGTCCTAGCAGGGGTACCAGACCAGCCAGAGGC CCCGCAGCCAGGTCCAAACACCACTGCGGCCCCTGTGGACTCAGGGCCCAAGGCTGGGCTGGCTCCAGAA ACCACAGAGACCCCGGCTGGGGCCTCAGAAACAGCCCAGGCCACAGACCTCAGCTTAAGCCCAGGAGGGG AATCAAAGGCCAACTGCAGCCCCGAAGACCCATGCCAAGAAACAGTGTCCAAACCAGAAGTGAGCAAAGA GGCCACTGCAGACCAGGGGTCCAGGCTGGAGTCTGCAGCCCCACCTGAACCAGCCCCAGAGCCTGCTCCC CAACCAGACCCCCGGCCAGATTCCCAGCCTACCCCCAAGCCAGCCCTTCAACCAGAGCTCCCTACCCAGG AGGACCCCACCCCTGAGATTCTGTCTGAGAGTGTAGGGGAAAAGCAAGAGAATGGGGCAGTGGTGCCCCT GCAGGCTGGTGATGGGGAAGAGGGCCCAGCCCCTGAGCCTCACTCACCACCCTCAAAAAAATCCCCCCCA GCCAATGGGGCCCCCCCCCGAGTGCTGCAGCAGCTGGTTGAGGAGGATCGAATGAGAAGGGCACACAGTG GGCATCCAGGATCTCCCCGAGGTAGCCTGAGCCGCCACCCCAGCTCCCAGCTGGCAGGTCCTGGGGTGGA GGGGGGTGAAGGCACCCAGAAACCTCGGGACTACATCATCCTTGCCATCCTGTCCTGCTTCTGCCCCATG TGGCCTGTCAACATCGTGGCCTTCGCTTATGCTGTCATGGTGAGCCCCATGGGACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233691 representing NM_001256443 Red=Cloning site Green=Tags(s) MAASSSEISEMKGVEESPKVPGEGPGHSEAETGPPQVLAGVPDQPEAPQPGPNTTAAPVDSGPKAGLAPE TTETPAGASETAQATDLSLSPGGESKANCSPEDPCQETVSKPEVSKEATADQGSRLESAAPPEPAPEPAP QPDPRPDSQPTPKPALQPELPTQEDPTPEILSESVGEKQENGAVVPLQAGDGEEGPAPEPHSPPSKKSPP ANGAPPRVLQQLVEEDRMRRAHSGHPGSPRGSLSRHPSSQLAGPGVEGGEGTQKPRDYIILAILSCFCPM WPVNIVAFAYAVMVSPMGP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256443
ORF Size	897 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256443.1, NP_001243372.1
RefSeq Size	3195 bp
RefSeq ORF	900 bp
Locus ID	112476
UniProt ID	Q7Z6L0
Protein Families	Transmembrane
MW	31.1 kDa
Gene Summary	This gene encodes a transmembrane protein containing a proline-rich domain in its N-terminal half. Studies in mice suggest that it is predominantly expressed in brain and spinal cord in embryonic and postnatal stages. Mutations in this gene are associated with episodic kinesigenic dyskinesia-1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

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