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KDM1A (NM_001009999) Human Tagged ORF Clone
CAT＃: RC230555
- TrueORF®
KDM1A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 1
ORF Plasmid: tGFP
"NM_001009999" in other vectors (7)
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CNY 9,624.00
| Cited in 1 publication. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AOF2; BHC110; CPRF; KDM1; LSD1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230555 representing NM_001009999
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTATCTGGGAAGAAGGCGGCAGCCGCGGCGGCGGCGGCTGCAGCGGCAGCAACCGGGACGGAGGCTG GCCCTGGGACAGCAGGCGGCTCCGAGAACGGGTCTGAGGTGGCCGCGCAGCCCGCGGGCCTGTCGGGCCC AGCCGAGGTCGGGCCGGGGGCGGTGGGGGAGCGCACACCCCGCAAGAAAGAGCCTCCGCGGGCCTCGCCC CCCGGGGGCCTGGCGGAACCGCCGGGGTCCGCAGGGCCTCAGGCCGGCCCTACTGTCGTGCCTGGGTCTG CGACCCCCATGGAAACTGGAATAGCAGAGACTCCGGAGGGGCGTCGGACCAGCCGGCGCAAGCGGGCGAA GGTAGAGTACAGAGAGATGGATGAAAGCTTGGCCAACCTCTCAGAAGATGAGTATTATTCAGAAGAAGAG AGAAATGCCAAAGCAGAGAAGGAAAAGAAGCTTCCCCCACCACCCCCTCAAGCCCCACCTGAGGAAGAAA ATGAAAGTGAGCCTGAAGAACCATCGGGGCAAGCAGGAGGACTTCAAGACGACAGTTCTGGAGGGTATGG AGACGGCCAAGCATCAGGTGTGGAGGGCGCAGCTTTCCAGAGCCGACTTCCTCATGACCGGATGACTTCT CAAGAAGCAGCCTGTTTTCCAGATATTATCAGTGGACCACAACAGACCCAGAAGGTTTTTCTTTTCATTA GAAACCGCACACTGCAGTTGTGGTTGGATAATCCAAAGATTCAGCTGACATTTGAGGCTACTCTCCAACA ATTAGAAGCACCTTATAACAGTGATACTGTGCTTGTCCACCGAGTTCACAGTTATTTAGAGCGTCATGGT CTTATCAACTTCGGCATCTATAAGAGGATAAAACCCCTACCAACTAAAAAGACAGGAAAGGTAATTATTA TAGGCTCTGGGGTCTCAGGCTTGGCAGCAGCTCGACAGTTACAAAGTTTTGGAATGGATGTCACACTTTT GGAAGCCAGGGATCGTGTGGGTGGACGAGTTGCCACATTTCGCAAAGGAAACTATGTAGCTGATCTTGGA GCCATGGTGGTAACAGGTCTTGGAGGGAATCCTATGGCTGTGGTCAGCAAACAAGTAAATATGGAACTGG CCAAGATCAAGCAAAAATGCCCACTTTATGAAGCCAACGGACAAGCTGACACTGTCAAGGTTCCTAAAGA GAAAGATGAAATGGTAGAGCAAGAGTTTAACCGGTTGCTAGAAGCTACATCTTACCTTAGTCATCAACTA GACTTCAATGTCCTCAATAATAAGCCTGTGTCCCTTGGCCAGGCATTGGAAGTTGTCATTCAGTTACAAG AGAAGCATGTCAAAGATGAGCAGATTGAACATTGGAAGAAGATAGTGAAAACTCAGGAAGAATTGAAAGA ACTTCTTAATAAGATGGTAAATTTGAAAGAGAAAATTAAAGAACTCCATCAGCAATACAAAGAAGCATCT GAAGTAAAGCCACCCAGAGATATTACTGCCGAGTTCTTAGTGAAAAGCAAACACAGGGATCTGACCGCCC TATGCAAGGAATATGATGAATTAGCTGAAACACAAGGAAAGCTAGAAGAAAAACTTCAGGAGTTGGAAGC GAATCCCCCAAGTGATGTATATCTCTCATCAAGAGACAGACAAATACTTGATTGGCATTTTGCAAATCTT GAATTTGCTAATGCCACACCTCTCTCAACTCTCTCCCTTAAGCACTGGGATCAGGATGATGACTTTGAGT TCACTGGCAGCCACCTGACAGTAAGGAATGGCTACTCGTGTGTGCCTGTGGCTTTAGCAGAAGGCCTAGA CATTAAACTGAATACAGCAGTGCGACAGGTTCGCTACACGGCTTCAGGATGTGAAGTGATAGCTGTGAAT ACCCGCTCCACGAGTCAAACCTTTATTTATAAATGCGACGCAGTTCTCTGTACCCTTCCCCTGGGTGTGC TGAAGCAGCAGCCACCAGCCGTTCAGTTTGTGCCACCTCTCCCTGAGTGGAAAACATCTGCAGTCCAAAG GATGGGATTTGGCAACCTTAACAAGGTGGTGTTGTGTTTTGATCGGGTGTTCTGGGATCCAAGTGTCAAT TTGTTCGGGCATGTTGGCAGTACGACTGCCAGCAGGGGTGAGCTCTTCCTCTTCTGGAACCTCTATAAAG CTCCAATACTGTTGGCACTAGTGGCAGGAGAAGCTGCTGGTATCATGGAAAACATAAGTGACGATGTGAT TGTTGGCCGATGCCTGGCCATTCTCAAAGGGATTTTTGGTAGCAGTGCAGTACCTCAGCCCAAAGAAACT GTGGTGTCTCGTTGGCGTGCTGATCCCTGGGCTCGGGGCTCTTATTCCTATGTTGCTGCAGGATCATCTG GAAATGACTATGATTTAATGGCTCAGCCAATCACTCCTGGCCCCTCGATTCCAGGTGCCCCACAGCCGAT TCCACGACTCTTCTTTGCGGGAGAACATACGATCCGTAACTACCCAGCCACAGTGCATGGTGCTCTGCTG AGTGGGCTGCGAGAAGCGGGAAGAATTGCAGACCAGTTTTTGGGGGCCATGTATACGCTGCCTCGCCAGG CCACACCAGGTGTTCCTGCACAGCAGTCCCCAAGCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230555 representing NM_001009999
Red=Cloning site Green=Tags(s) MLSGKKAAAAAAAAAAAATGTEAGPGTAGGSENGSEVAAQPAGLSGPAEVGPGAVGERTPRKKEPPRASP PGGLAEPPGSAGPQAGPTVVPGSATPMETGIAETPEGRRTSRRKRAKVEYREMDESLANLSEDEYYSEEE RNAKAEKEKKLPPPPPQAPPEEENESEPEEPSGQAGGLQDDSSGGYGDGQASGVEGAAFQSRLPHDRMTS QEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHG LINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLG AMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQADTVKVPKEKDEMVEQEFNRLLEATSYLSHQL DFNVLNNKPVSLGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEAS EVKPPRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANL EFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVN TRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVN LFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKET VVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALL SGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001009999 |
| ORF Size | 2628 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001009999.3 |
| RefSeq Size | 3125 bp |
| RefSeq ORF | 2631 bp |
| Locus ID | 23028 |
| UniProt ID | O60341 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 95.6 kDa |
| Gene Summary | This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression
,null,
International Journal of Molecular Medicine
,PubMed ID 29207083
[KDM1A]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230555L1 | Lenti-ORF clone of KDM1A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 1 |
CNY 12,024.00 |
|
| RC230555L2 | Lenti-ORF clone of KDM1A (mGFP-tagged)-Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 1 |
CNY 5,890.00 |
|
| RC230555L3 | Lenti-ORF clone of KDM1A (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 1 |
CNY 5,890.00 |
|
| RC230555L4 | Lenti-ORF clone of KDM1A (mGFP-tagged)-Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 1 |
CNY 12,024.00 |
|
| RG230555 | KDM1A (tGFP-tagged) - Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 1 |
CNY 11,224.00 |
|
| SC301471 | KDM1A (untagged)-Human lysine (K)-specific demethylase 1A (KDM1A), transcript variant 1 |
CNY 9,632.00 |
|
| SC329193 | KDM1A (untagged)-Human lysine (K)-specific demethylase 1A (KDM1A) transcript variant 1 |
CNY 10,264.00 |
