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PTPN12 (NM_001131009) Human Tagged ORF Clone
CAT＃: RC226061
- TrueORF®
PTPN12 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 12 (PTPN12), transcript variant 3
ORF Plasmid: tGFP
"NM_001131009" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PTP-PEST; PTPG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC226061 representing NM_001131009
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGCCGAGAATTTGAGATGGGAAGGAAAAAATGTGAGCGCTATTGGCCTTTGTATGGAGAAGACC CCATAACGTTTGCACCATTTAAAATTTCTTGTGAGGATGAACAAGCAAGAACAGACTACTTCATCAGGAC ACTCTTACTTGAATTTCAAAATGAATCTCGTAGGCTGTATCAGTTTCATTATGTGAACTGGCCAGACCAT GATGTTCCTTCATCATTTGATTCTATTCTGGACATGATAAGCTTAATGAGGAAATATCAAGAACATGAAG ATGTTCCTATTTGTATTCATTGCAGTGCAGGCTGTGGAAGAACAGGTGCCATTTGTGCCATAGATTATAC GTGGAATTTACTAAAAGCTGGGAAAATACCAGAGGAATTTAATGTATTTAATTTAATACAAGAAATGAGA ACACAAAGGCATTCTGCAGTACAAACAAAGGAGCAATATGAACTTGTTCATAGAGCTATTGCCCAACTGT TTGAAAAACAGCTACAACTATATGAAATTCATGGAGCTCAGAAAATTGCTGATGGAGTGAATGAAATTAA CACTGAAAACATGGTCAGCTCCATAGAGCCTGAAAAACAAGATTCTCCTCCTCCAAAACCACCAAGGACC CGCAGTTGCCTTGTTGAAGGGGATGCTAAAGAAGAAATACTGCAGCCACCGGAACCTCATCCAGTGCCAC CCATCTTGACACCTTCTCCCCCTTCAGCTTTTCCAACAGTCACTACTGTGTGGCAGGACAATGATAGATA CCATCCAAAGCCAGTGTTGCATATGGTTTCATCAGAACAACATTCAGCAGACCTCAACAGAAACTATAGT AAATCAACAGAACTTCCAGGGAAAAATGAATCAACAATTGAACAGATAGATAAAAAATTGGAACGAAATT TAAGTTTTGAGATTAAGAAGGTCCCTCTCCAAGAGGGACCAAAAAGTTTTGATGGGAACACACTTTTGAA TAGGGGACATGCAATTAAAATTAAATCTGCTTCACCTTGTATAGCTGATAAAATCTCTAAGCCACAGGAA TTAAGTTCAGATCTAAATGTCGGTGATACTTCCCAGAATTCTTGTGTGGACTGCAGTGTAACACAATCAA ACAAAGTTTCAGTTACTCCACCAGAAGAATCCCAGAATTCAGACACACCTCCAAGGCCAGACCGCTTGCC TCTTGATGAGAAAGGACATGTAACGTGGTCATTTCATGGACCTGAAAATGCCATACCCATACCTGATTTA TCTGAAGGCAATTCCTCAGATATCAACTATCAAACTAGGAAAACTGTGAGTTTAACACCAAGTCCTACAA CACAAGTTGAAACACCTGATCTTGTGGATCATGATAACACTTCACCACTCTTCAGAACACCCCTCAGTTT TACTAATCCACTTCACTCTGATGACTCAGACTCAGATGAAAGAAACTCTGATGGTGCTGTGACCCAGAAT AAAACTAATATTTCAACAGCAAGTGCCACAGTTTCTGCTGCCACTAGTACTGAAAGCATTTCTACTAGGA AAGTATTGCCAATGTCCATTGCTAGACATAATATAGCAGGAACAACACATTCAGGTGCTGAAAAAGATGT TGATGTTAGTGAAGATTCACCTCCTCCCCTACCTGAAAGAACTCCTGAATCGTTTGTGTTAGCAAGTGAA CATAATACACCTGTAAGATCGGAATGGAGTGAACTTCAAAGTCAGGAACGATCTGAACAAAAAAAGTCTG AAGGCTTGATAACCTCTGAAAATGAGAAATGTGATCATCCAGCGGGAGGTATTCACTATGAAATGTGCAT AGAATGTCCACCTACTTTCAGTGACAAGAGAGAACAAATATCAGAAAATCCAACAGAAGCCACAGATATT GGTTTTGGTAATCGATGTGGAAAACCCAAAGGACCAAGAGATCCACCTTCAGAATGGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226061 representing NM_001131009
Red=Cloning site Green=Tags(s) MACREFEMGRKKCERYWPLYGEDPITFAPFKISCEDEQARTDYFIRTLLLEFQNESRRLYQFHYVNWPDH DVPSSFDSILDMISLMRKYQEHEDVPICIHCSAGCGRTGAICAIDYTWNLLKAGKIPEEFNVFNLIQEMR TQRHSAVQTKEQYELVHRAIAQLFEKQLQLYEIHGAQKIADGVNEINTENMVSSIEPEKQDSPPPKPPRT RSCLVEGDAKEEILQPPEPHPVPPILTPSPPSAFPTVTTVWQDNDRYHPKPVLHMVSSEQHSADLNRNYS KSTELPGKNESTIEQIDKKLERNLSFEIKKVPLQEGPKSFDGNTLLNRGHAIKIKSASPCIADKISKPQE LSSDLNVGDTSQNSCVDCSVTQSNKVSVTPPEESQNSDTPPRPDRLPLDEKGHVTWSFHGPENAIPIPDL SEGNSSDINYQTRKTVSLTPSPTTQVETPDLVDHDNTSPLFRTPLSFTNPLHSDDSDSDERNSDGAVTQN KTNISTASATVSAATSTESISTRKVLPMSIARHNIAGTTHSGAEKDVDVSEDSPPPLPERTPESFVLASE HNTPVRSEWSELQSQERSEQKKSEGLITSENEKCDHPAGGIHYEMCIECPPTFSDKREQISENPTEATDI GFGNRCGKPKGPRDPPSEWT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001131009 |
| ORF Size | 1950 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001131009.2 |
| RefSeq Size | 3026 bp |
| RefSeq ORF | 1953 bp |
| Locus ID | 5782 |
| UniProt ID | Q05209 |
| Protein Families | Druggable Genome, Phosphatase |
| MW | 72.8 kDa |
| Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226061L3 | Lenti-ORF clone of PTPN12 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 12 (PTPN12), transcript variant 3 |
CNY 5,890.00 |
|
| RC226061L4 | Lenti-ORF clone of PTPN12 (mGFP-tagged)-Human protein tyrosine phosphatase, non-receptor type 12 (PTPN12), transcript variant 3 |
CNY 5,890.00 |
|
| RG226061 | PTPN12 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 12 (PTPN12), transcript variant 3 |
CNY 4,370.00 |
|
| SC325257 | PTPN12 (untagged)-Human protein tyrosine phosphatase, non-receptor type 12 (PTPN12), transcript variant 3 |
CNY 7,220.00 |
