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SLC12A6 (NM_005135) Human Tagged ORF Clone
CAT＃: RC224578
- TrueORF®
SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 2
ORF Plasmid: tGFP
"NM_005135" in other vectors (4)
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CNY 10,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACCPN; KCC3; KCC3A; KCC3B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224578 representing NM_005135
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACATTTTACTGTGACTAAGGTAGAGGACCCAGAGGAGGGGGCAGCGGCTTCGATCTCTCAAGAGC CTAGTTTAGCAGACATAAAAGCCCGGATTCAGGATTCAGATGAACCAGACCTGAGTCAGAACTCCATCAC AGGGGAACACAGCCAACTGTTAGACGACGGACATAAGAAAGCTCGAAATGCTTATCTCAATAATTCCAAT TATGAAGAAGGAGATGAATATTTTGATAAAAATTTGGCACTCTTTGAGGAAGAAATGGACACCAGACCGA AGGTGTCTTCCCTCCTCAACCGCATGGCCAATTACACTAATCTGACTCAAGGAGCAAAGGAACATGAAGA GGCAGAAAACATCACTGAAGGGAAAAAGAAGCCCACCAAGACCCCCCAAATGGGTACCTTCATGGGTGTC TACCTCCCATGTCTACAAAATATTTTTGGAGTGATCCTTTTTTTACGCCTTACATGGGTGGTGGGCACAG CTGGAGTTCTTCAGGCTTTTGCAATTGTCCTTATCTGCTGCTGCTGTACAATGTTGACTGCTATCTCCAT GAGTGCCATTGCCACTAATGGAGTGGTGCCAGCTGGGGGCTCATACTTTATGATTTCCCGGGCACTGGGC CCAGAGTTTGGTGGGGCTGTTGGCCTCTGCTTTTATCTTGGTACCACATTTGCAGCAGCCATGTACATCC TTGGTGCCATTGAAATCTTTCTGGTCTATATCGTCCCCCGAGCTGCCATCTTTCACAGTGATGACGCACT CAAGGAATCAGCAGCCATGCTAAATAACATGCGTGTCTACGGCACAGCTTTCTTGGTCCTTATGGTATTA GTGGTATTTATCGGCGTACGCTATGTGAACAAGTTTGCCTCACTTTTCCTGGCCTGTGTCATTGTGTCCA TCTTGGCCATCTATGCTGGAGCCATCAAGTCTTCTTTTGCTCCTCCACACTTCCCGGTCTGCATGCTGGG TAACCGCACCCTTTCATCAAGACACATTGACGTTTGCTCTAAGACCAAGGAAATTAACAACATGACAGTC CCATCAAAGTTATGGGGATTCTTCTGTAACTCGAGTCAATTTTTCAATGCCACCTGTGATGAATACTTTG TTCACAATAACGTCACTTCAATCCAGGGCATTCCTGGATTGGCTAGTGGTATAATTACAGAGAATCTTTG GAGTAATTACCTACCCAAGGGAGAGATCATCGAAAAGCCTTCAGCCAAATCTTCTGATGTCTTAGGCAGC TTAAACCATGAATATGTTCTTGTTGACATCACCACCTCCTTCACGCTTCTGGTGGGAATCTTCTTTCCCT CTGTTACAGGTATCATGGCTGGATCAAACAGATCTGGAGATCTGAAAGATGCTCAGAAGTCTATTCCGAT TGGTACTATCCTTGCCATCCTGACCACCTCCTTTGTTTATTTAAGCAATGTTGTCCTTTTTGGTGCATGT ATTGAAGGGGTTGTTCTCAGAGACAAGTTCGGTGATGCTGTGAAAGGTAATTTGGTGGTAGGCACCTTAT CTTGGCCATCCCCATGGGTGATTGTTATTGGCTCCTTCTTTTCAACATGTGGGGCTGGACTTCAGAGCCT CACAGGTGCACCGAGGCTGCTACAAGCTATTGCCAAGGATAACATCATACCGTTTCTGAGGGTTTTTGGC CACAGCAAAGCCAATGGGGAACCTACCTGGGCTTTACTTCTAACTGCTGCCATTGCAGAGCTTGGAATAC TCATTGCCTCCCTGGATCTTGTGGCCCCAATTCTTTCCATGTTTTTTCTCATGTGTTACCTCTTTGTAAA CTTGGCATGTGCCTTGCAAACATTACTTCGAACACCCAACTGGAGACCCCGATTCCGCTACTACCATTGG GCCCTTTCTTTCATGGGAATGAGTATCTGTCTGGCTCTGATGTTCATTTCTTCCTGGTATTATGCCATTG TAGCCATGGTAATAGCTGGTATGATCTACAAGTACATTGAATACCAAGGAGCTGAGAAAGAATGGGGTGA TGGTATCCGTGGGCTGTCCCTCAGTGCAGCCCGGTTTGCTTTGCTTCGATTGGAGGAAGGACCTCCACAC ACTAAAAACTGGAGGCCTCAGTTGCTTGTATTACTGAAACTAGATGAAGACTTACATGTCAAGCATCCTC GCCTCCTCACCTTTGCCTCACAGCTCAAAGCAGGAAAAGGTCTCACTATTGTGGGCTCTGTCATCGTGGG GAACTTCCTAGAGAACTACGGTGAAGCTTTAGCTGCTGAGCAGACCATAAAGCACCTAATGGAGGCAGAG AAGGTAAAAGGATTCTGCCAGCTGGTGGTGGCCGCCAAGCTGAGAGAGGGCATTTCCCACCTCATCCAGT CATGTGGCCTTGGGGGCATGAAGCACAACACGGTGGTGATGGGCTGGCCTAATGGCTGGCGTCAAAGCGA AGATGCCCGCGCTTGGAAGACTTTTATTGGCACAGTTCGAGTGACAACTGCTGCCCATCTTGCACTGCTG GTGGCTAAAAACATCTCCTTCTTTCCCAGCAATGTGGAGCAATTTTCTGAGGGCAACATTGATGTGTGGT GGATTGTGCATGATGGGGGGATGCTTATGCTACTACCATTCCTACTGAAACAGCACAAGGTGTGGCGAAA GTGCAGCATACGGATCTTCACAGTAGCCCAATTAGAAGACAACAGTATCCAAATGAAGAAGGACCTAGCC ACCTTCCTATATCACTTACGCATTGAGGCGGAGGTAGAAGTGGTGGAGATGCATGACAGTGATATATCAG CATATACTTACGAGCGCACTTTGATGATGGAACAAAGGTCCCAGATGCTCCGGCACATGCGGCTATCCAA AACAGAGCGAGACAGAGAGGCACAATTGGTGAAAGACCGAAACTCAATGCTACGATTGACCAGCATTGGC TCTGATGAGGACGAAGAGACAGAAACCTATCAGGAGAAGGTGCACATGACTTGGACAAAAGACAAGTACA TGGCATCCCGGGGACAAAAAGCGAAGTCAATGGAAGGATTCCAGGACCTGCTTAACATGCGTCCGGACCA GTCCAATGTGAGGCGGATGCATACAGCAGTGAAACTCAACGAGGTTATAGTTAACAAGTCCCATGAAGCA AAGCTGGTTTTATTGAATATGCCAGGGCCACCCCGAAACCCTGAGGGTGATGAAAACTACATGGAGTTCC TAGAGGTGCTTACCGAGGGACTAGAGCGAGTCCTACTTGTCCGGGGTGGTGGCAGTGAAGTGATCACCAT TTATTCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224578 representing NM_005135
Red=Cloning site Green=Tags(s) MPHFTVTKVEDPEEGAAASISQEPSLADIKARIQDSDEPDLSQNSITGEHSQLLDDGHKKARNAYLNNSN YEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGV YLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALG PEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVL VVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTV PSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGS LNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGAC IEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFG HSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHW ALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPH TKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAE KVKGFCQLVVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALL VAKNISFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLA TFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIG SDEDEETETYQEKVHMTWTKDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEA KLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005135 |
| ORF Size | 3297 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005135.2, NP_005126.1 |
| RefSeq Size | 7327 bp |
| RefSeq ORF | 3300 bp |
| Locus ID | 9990 |
| UniProt ID | Q9UHW9 |
| Domains | KCl_Cotrans_1 |
| Protein Families | Transmembrane |
| MW | 122.1 kDa |
| Gene Summary | This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224578L3 | Lenti-ORF clone of SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 2 |
CNY 12,830.00 |
|
| RC224578L4 | Lenti-ORF clone of SLC12A6 (mGFP-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 2 |
CNY 12,830.00 |
|
| RG224578 | SLC12A6 (tGFP-tagged) - Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 2 |
CNY 11,970.00 |
|
| SC309985 | SLC12A6 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 2 |
CNY 21,280.00 |
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