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CPEB4 (NM_030627) Human Tagged ORF Clone
CAT＃: RC221803
CPEB4 (Myc-DDK-tagged)-Human cytoplasmic polyadenylation element binding protein 4 (CPEB4)
ORF Plasmid: tGFP
"NM_030627" in other vectors (6)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CPE-BP4; hCPEB-4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221803 representing NM_030627
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGATTACGGGTTTGGAGTGCTAGTGCAAAGCAATACTGGGAATAAATCTGCTTTTCCAGTCAGAT TCCATCCACATCTGCAGCCTCCACACCATCACCAAAATGCCACCCCCAGCCCTGCTGCTTTTATAAATAA TAACACAGCTGCCAATGGCAGCAGTGCTGGGTCAGCTTGGCTTTTTCCTGCTCCAGCTACCCATAACATT CAGGATGAGATCTTGGGGTCAGAAAAAGCAAAAAGTCAGCAACAGGAACAGCAAGACCCCTTAGAAAAGC AGCAGCTTTCCCCAAGTCCAGGTCAGGAAGCTGGAATACTGCCTGAAACAGAGAAGGCAAAATCAGAAGA AAATCAAGGGGACAATTCTTCGGAAAATGGCAATGGGAAGGAGAAAATAAGGATCGAATCTCCAGTGTTG ACAGGGTTTGATTATCAAGAAGCCACTGGGCTAGGTACTTCAACCCAACCCTTGACATCTAGCGCATCGT CTCTTACTGGTTTCAGTAACTGGTCAGCAGCGATAGCGCCTTCCTCCTCTACAATAATCAATGAAGATGC AAGTTTCTTTCACCAGGGAGGGGTCCCTGCTGCTTCGGCTAATAACGGTGCTCTGTTGTTTCAAAATTTC CCCCATCATGTCAGCCCTGGCTTTGGAGGCAGCTTCTCTCCTCAGATCGGGCCTCTCTCACAGCACCACC CACATCACCCTCATTTCCAGCATCATCACAGCCAGCATCAGCAGCAAAGGAGGTCTCCTGCCAGTCCCCA TCCCCCACCCTTCACACATAGAAATGCTGCTTTTAACCAGCTGCCTCATTTGGCGAATAATCTTAACAAA CCCCCCTCTCCGTGGAGCAGCTACCAGAGTCCGTCACCAACACCCTCCTCTTCCTGGAGCCCGGGCGGTG GTGGATATGGTGGCTGGGGAGGTTCCCAAGGCCGAGATCACCGCAGAGGGCTGAATGGTGGAATAACGCC CCTGAACTCCATCTCGCCTTTGAAGAAAAATTTTGCAAGCAATCATATTCAGCTCCAGAAGTATGCTCGC CCCAGCTCTGCCTTTGCACCTAAATCCTGGATGGAAGATAGCTTGAACAGGGCTGACAACATTTTTCCTT TTCCGGATCGCCCCAGGACATTCGACATGCACTCACTGGAGAGTTCACTCATTGACATAATGAGAGCTGA AAATGATACCATTAAAGGTCGTCTAAACTATTCATATCCAGGATCCGATAGCTCTCTGCTTATTAATGCA AGGACATATGGGCGAAGGAGAGGTCAGTCTTCACTGTTTCCAATGGAAGATGGATTCTTGGATGATGGCC GTGGGGATCAGCCTCTTCATAGTGGCCTGGGTTCACCTCACTGCTTCAGTCACCAGAATGGGGAAAGAGT GGAACGATATTCTCGAAAGGTGTTTGTAGGCGGATTGCCTCCAGACATTGATGAAGATGAGATCACAGCT AGTTTTCGTCGCTTTGGCCCTCTGATTGTGGATTGGCCTCATAAAGCTGAGAGCAAATCCTATTTTCCTC CTAAAGGCTATGCATTCCTGCTGTTTCAAGATGAAAGCTCTGTGCAGGCTCTCATTGATGCATGCATTGA AGAAGATGGAAAACTCTACCTTTGTGTATCAAGTCCCACTATCAAGGATAAGCCAGTCCAGATTCGGCCT TGGAATCTCAGTGACAGTGACTTTGTGATGGATGGTTCACAGCCACTTGACCCACGAAAAACTATATTTG TTGGTGGTGTTCCTCGACCATTACGAGCTGTGGAGCTTGCGATGATAATGGATCGGCTATATGGAGGTGT GTGCTACGCTGGGATTGATACCGACCCTGAGCTAAAATACCCAAAAGGAGCTGGGAGAGTTGCGTTCTCT AATCAACAGAGTTACATAGCTGCTATCAGTGCCCGCTTTGTTCAGCTGCAGCATGGAGAGATAGATAAAC GGGTGGAAGTTAAGCCATATGTCTTGGATGATCAGCTGTGTGATGAATGTCAGGGGGCCCGTTGTGGGGG GAAATTTGCTCCATTTTTCTGTGCTAATGTTACCTGTCTGCAGTATTACTGTGAATATTGCTGGGCTGCT ATCCATTCTCGTGCTGGCAGGGAATTCCACAAGCCCCTGGTGAAGGAAGGCGGTGACCGCCCTCGGCATA TTTCATTCCGCTGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221803 representing NM_030627
Red=Cloning site Green=Tags(s) MGDYGFGVLVQSNTGNKSAFPVRFHPHLQPPHHHQNATPSPAAFINNNTAANGSSAGSAWLFPAPATHNI QDEILGSEKAKSQQQEQQDPLEKQQLSPSPGQEAGILPETEKAKSEENQGDNSSENGNGKEKIRIESPVL TGFDYQEATGLGTSTQPLTSSASSLTGFSNWSAAIAPSSSTIINEDASFFHQGGVPAASANNGALLFQNF PHHVSPGFGGSFSPQIGPLSQHHPHHPHFQHHHSQHQQQRRSPASPHPPPFTHRNAAFNQLPHLANNLNK PPSPWSSYQSPSPTPSSSWSPGGGGYGGWGGSQGRDHRRGLNGGITPLNSISPLKKNFASNHIQLQKYAR PSSAFAPKSWMEDSLNRADNIFPFPDRPRTFDMHSLESSLIDIMRAENDTIKGRLNYSYPGSDSSLLINA RTYGRRRGQSSLFPMEDGFLDDGRGDQPLHSGLGSPHCFSHQNGERVERYSRKVFVGGLPPDIDEDEITA SFRRFGPLIVDWPHKAESKSYFPPKGYAFLLFQDESSVQALIDACIEEDGKLYLCVSSPTIKDKPVQIRP WNLSDSDFVMDGSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFS NQQSYIAAISARFVQLQHGEIDKRVEVKPYVLDDQLCDECQGARCGGKFAPFFCANVTCLQYYCEYCWAA IHSRAGREFHKPLVKEGGDRPRHISFRWN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030627 |
| ORF Size | 2187 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030627.4 |
| RefSeq Size | 6930 bp |
| RefSeq ORF | 2190 bp |
| Locus ID | 80315 |
| UniProt ID | Q17RY0 |
| MW | 80 kDa |
| Gene Summary | Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3' UTR (PubMed:24990967). RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed:24990967). Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (By similarity). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation (PubMed:26398195). Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT (PubMed:22138752). Stimulates proliferation of melanocytes (PubMed:27857118). In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Functional Integration of mRNA Translational Control Programs
,MacNicol, MC;Cragle, CE;Arumugam, K;Fosso, B;Pesole, G;MacNicol, AM;,
Biomolecules
,PubMed ID 26197342
[CPEB4]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221803L1 | Lenti-ORF clone of CPEB4 (Myc-DDK-tagged)-Human cytoplasmic polyadenylation element binding protein 4 (CPEB4) |
CNY 5,890.00 |
|
| RC221803L2 | Lenti-ORF clone of CPEB4 (mGFP-tagged)-Human cytoplasmic polyadenylation element binding protein 4 (CPEB4) |
CNY 5,890.00 |
|
| RC221803L3 | Lenti-ORF clone of CPEB4 (Myc-DDK-tagged)-Human cytoplasmic polyadenylation element binding protein 4 (CPEB4) |
CNY 5,890.00 |
|
| RC221803L4 | Lenti-ORF clone of CPEB4 (mGFP-tagged)-Human cytoplasmic polyadenylation element binding protein 4 (CPEB4) |
CNY 5,890.00 |
|
| RG221803 | CPEB4 (tGFP-tagged) - Human cytoplasmic polyadenylation element binding protein 4 (CPEB4) |
CNY 4,370.00 |
|
| SC107390 | CPEB4 (untagged)-Human cytoplasmic polyadenylation element binding protein 4 (CPEB4) |
CNY 5,344.00 |
