PRDM9 (NM_020227) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KMT8B; MEISETZ; MSBP3; PFM6; ZNF899
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC218187 representing NM_020227 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCTGAAAAGTCCCAAGAGGAGAGCCCAGAAGAAGACACAGAGAGAACAGAGCGGAAGCCCATGG TCAAAGATGCCTTCAAAGACATTTCCATATACTTCACCAAGGAAGAATGGGCAGAGATGGGAGACTGGGA GAAAACTCGCTATAGGAATGTGAAAAGGAACTATAATGCACTGATTACTATAGGTCTCAGAGCCACTCGA CCAGCTTTCATGTGTCACCGAAGGCAGGCCATCAAACTCCAGGTGGATGACACAGAAGATTCTGATGAAG AATGGACCCCTAGGCAGCAAGTCAAACCTCCTTGGATGGCCTTAAGAGTGGAACAGCGTAAACACCAGAA GGGAATGCCCAAGGCGTCATTCAGTAATGAATCTAGTTTGAAAGAATTGTCAAGAACAGCAAATTTACTG AATGCAAGTGGCTCAGAGCAGGCTCAGAAACCAGTGTCCCCTTCTGGAGAAGCAAGTACCTCTGGACAGC ACTCAAGACTAAAACTGGAACTCAGGAAGAAGGAGACTGAAAGAAAGATGTATAGCCTGCGAGAAAGAAA GGGTCATGCATACAAAGAGGTCAGCGAGCCGCAGGATGATGATTACCTCTATTGTGAGATGTGTCAGAAC TTCTTCATTGACAGCTGTGCTGCCCATGGGCCCCCTACATTTGTAAAGGACAGTGCAGTGGACAAGGGGC ACCCCAACCGTTCAGCCCTCAGTCTGCCCCCAGGGCTGAGAATTGGGCCATCAGGCATCCCTCAGGCTGG GCTTGGAGTATGGAATGAGGCATCTGATCTGCCGCTGGGTCTGCACTTTGGCCCTTATGAGGGCCGAATT ACAGAAGACGAAGAGGCAGCCAACAATGGATACTCCTGGCTGATCACCAAGGGGAGAAACTGCTATGAGT ATGTGGATGGAAAAGATAAATCCTGGGCCAACTGGATGAGGTATGTGAACTGTGCCCGGGATGATGAAGA GCAGAACCTGGTGGCCTTCCAGTACCACAGGCAGATCTTCTATAGAACCTGCCGAGTCATTAGGCCAGGC TGTGAACTGCTGGTCTGGTATGGGGATGAATACGGCCAGGAACTGGGCATCAAGTGGGGCAGCAAGTGGA AGAAAGAGCTCATGGCAGGGAGAGAACCAAAGCCAGAGATCCATCCATGTCCCTCATGCTGTCTGGCCTT TTCAAGTCAGAAATTTCTCAGTCAACATGTAGAACGCAATCACTCCTCTCAGAACTTCCCAGGACCATCT GCAAGAAAACTCCTCCAACCAGAGAATCCCTGCCCAGGGGATCAGAATCAGGAGCAGCAATATCCAGATC CACACAGCCGTAATGACAAAACCAAAGGTCAAGAGATCAAAGAAAGGTCCAAACTCTTGAATAAAAGGAC ATGGCAGAGGGAGATTTCAAGGGCCTTTTCTAGCCCACCCAAAGGACAAATGGGGAGCTGTAGAGTGGGA AAAAGAATAATGGAAGAAGAGTCCAGAACAGGCCAGAAAGTGAATCCAGGGAACACAGGCAAATTATTTG TGGGGGTAGGAATCTCAAGAATTGCAAAAGTCAAGTATGGAGAGTGTGGACAAGGTTTCAGTGTTAAATC AGATGTTATTACACACCAAAGGACACATACAGGGGAGAAGCTCTACGTCTGCAGGGAGTGTGGGCGGGGC TTTAGCTGGAAGTCACACCTCCTCATTCACCAGAGGATACACACAGGGGAGAAGCCCTATGTCTGCAGGG AGTGTGGGCGGGGCTTTAGCTGGCAGTCAGTCCTCCTCACTCACCAGAGGACACACACAGGGGAGAAGCC CTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGCCGGCAGTCAGTCCTCCTCACTCACCAGAGGAGACAC ACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGCCGGCAGTCAGTCCTCCTCACTC ACCAGAGGAGACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGCTGGCAGTC AGTCCTCCTCACTCACCAGAGGACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGC TTTAGCTGGCAGTCAGTCCTCCTCACTCACCAGAGGACACACACAGGGGAGAAGCCCTATGTCTGCAGGG AGTGTGGGCGGGGCTTTAGCAATAAGTCACACCTCCTCAGACACCAGAGGACACACACAGGGGAGAAGCC CTATGTCTGCAGGGAGTGTGGGCGGGGCTTTCGCGATAAGTCACACCTCCTCAGACACCAGAGGACACAC ACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGAGATAAGTCAAACCTCCTCAGTC ACCAGAGGACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGCAATAAGTC ACACCTCCTCAGACACCAGAGGACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGC TTTCGCAATAAGTCACACCTCCTCAGACACCAGAGGACACACACAGGGGAGAAGCCCTACGTCTGCAGGG AGTGTGGGCGGGGCTTTAGCGATAGGTCAAGCCTCTGCTATCACCAGAGGACACACACAGGGGAGAAGCC CTACGTCTGCAGGGAGGATGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC218187 representing NM_020227 Red=Cloning site Green=Tags(s) MSPEKSQEESPEEDTERTERKPMVKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNALITIGLRATR PAFMCHRRQAIKLQVDDTEDSDEEWTPRQQVKPPWMALRVEQRKHQKGMPKASFSNESSLKELSRTANLL NASGSEQAQKPVSPSGEASTSGQHSRLKLELRKKETERKMYSLRERKGHAYKEVSEPQDDDYLYCEMCQN FFIDSCAAHGPPTFVKDSAVDKGHPNRSALSLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRI TEDEEAANNGYSWLITKGRNCYEYVDGKDKSWANWMRYVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPG CELLVWYGDEYGQELGIKWGSKWKKELMAGREPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPS ARKLLQPENPCPGDQNQEQQYPDPHSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPPKGQMGSCRVG KRIMEEESRTGQKVNPGNTGKLFVGVGISRIAKVKYGECGQGFSVKSDVITHQRTHTGEKLYVCRECGRG FSWKSHLLIHQRIHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSRQSVLLTHQRRH TGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRG FSWQSVLLTHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRDKSHLLRHQRTH TGEKPYVCRECGRGFRDKSNLLSHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRG FRNKSHLLRHQRTHTGEKPYVCRECGRGFSDRSSLCYHQRTHTGEKPYVCREDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_020227
ORF Size	2682 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_020227.4
RefSeq Size	3691 bp
RefSeq ORF	2685 bp
Locus ID	56979
UniProt ID	Q9NQV7
MW	103.2 kDa
Gene Summary	The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described. [provided by RefSeq, Jul 2015]

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