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MEGF10 (NM_032446) Human Tagged ORF Clone
CAT＃: RC217883
MEGF10 (Myc-DDK-tagged)-Human multiple EGF-like-domains 10 (MEGF10)
ORF Plasmid: tGFP
"NM_032446" in other vectors (6)
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CNY 11,904.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EMARDD; SR-F3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217883 representing NM_032446
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTATTTCTTTGAACTCATGCCTGAGCTTTATTTGTTTATTGTTATGCCACTGGATTGGGACAGCAT CACCTCTGAATCTTGAAGACCCTAATGTGTGTAGCCACTGGGAAAGCTACTCAGTGACTGTGCAAGAGTC ATACCCACATCCCTTTGATCAAATTTACTACACGAGCTGCACTGACATTCTAAACTGGTTTAAATGCACG CGGCACAGAGTCAGCTATCGGACAGCCTATCGACATGGGGAGAAGACTATGTATAGGCGCAAGTCTCAGT GTTGTCCTGGATTTTATGAAAGCGGGGAAATGTGTGTCCCCCACTGTGCTGATAAATGTGTCCATGGTCG CTGTATTGCTCCAAACACCTGTCAGTGTGAGCCTGGCTGGGGAGGGACCAACTGCTCCAGTGCCTGCGAT GGTGATCACTGGGGTCCCCACTGCACCAGCCGGTGCCAGTGCAAAAATGGGGCTCTGTGCAACCCCATCA CCGGGGCTTGCCACTGTGCTGCGGGCTTCCGGGGCTGGCGCTGCGAGGACCGCTGTGAGCAGGGCACCTA TGGTAACGACTGTCATCAGAGATGCCAGTGCCAGAATGGAGCCACCTGCGACCACGTCACGGGGGAATGC CGCTGCCCACCAGGATACACCGGAGCCTTCTGTGAGGATCTTTGTCCTCCTGGTAAACATGGTCCACAGT GTGAGCAGAGATGCCCTTGTCAAAATGGAGGAGTGTGTCATCACGTCACTGGAGAATGCTCTTGCCCTTC TGGCTGGATGGGCACAGTGTGTGGTCAGCCTTGCCCCGAGGGTCGCTTTGGAAAGAACTGTTCCCAAGAA TGCCAGTGCCATAATGGAGGGACGTGTGATGCTGCCACAGGCCAATGTCATTGCAGTCCAGGATACACAG GGGAACGGTGCCAGGATGAGTGTCCTGTTGGGACCTATGGCGTTCTCTGTGCTGAGACCTGCCAGTGTGT CAACGGAGGGAAGTGTTACCACGTGAGCGGCGCATGCCTCTGTGAAGCAGGCTTTGCTGGCGAGCGCTGC GAAGCACGCCTGTGTCCTGAGGGGCTCTACGGCATCAAATGTGACAAACGGTGTCCCTGCCACCTGGAAA ACACTCATAGCTGTCACCCCATGTCTGGAGAGTGTGCCTGCAAGCCGGGCTGGTCAGGACTCTACTGTAA TGAGACATGTTCTCCTGGATTCTACGGGGAAGCTTGCCAGCAGATCTGCAGCTGCCAAAATGGGGCAGAC TGTGACAGTGTGACTGGAAAGTGCACCTGTGCCCCAGGATTCAAAGGAATTGACTGCTCTACCCCATGCC CTCTGGGAACCTATGGGATAAACTGTTCCTCTCGCTGTGGCTGTAAAAATGATGCAGTCTGCTCTCCTGT GGACGGGTCTTGTACTTGCAAGGCAGGCTGGCACGGGGTGGACTGCTCCATCAGATGTCCCAGTGGCACA TGGGGCTTTGGCTGTAACTTAACATGCCAGTGCCTCAACGGGGGAGCCTGCAACACCCTGGACGGGACCT GCACGTGTGCACCTGGATGGCGCGGGGAGAAATGCGAACTTCCCTGCCAGGATGGCACGTACGGGCTGAA CTGTGCTGAGCGCTGCGACTGCAGCCACGCAGATGGCTGCCACCCTACCACGGGCCATTGCCGCTGCCTC CCCGGATGGTCAGGTGTCCACTGTGACAGCGTGTGTGCTGAGGGACGCTGGGGCCCCAACTGCTCCCTGC CCTGCTACTGTAAAAATGGGGCTTCATGCTCCCCTGATGATGGCATCTGCGAGTGTGCACCAGGCTTCCG AGGCACCACTTGTCAGAGGATCTGCTCCCCTGGTTTTTATGGGCATCGCTGCAGCCAGACATGCCCACAG TGCGTTCACAGCAGCGGGCCCTGCCACCACATCACCGGCCTGTGTGACTGCTTGCCTGGCTTCACAGGCG CCCTCTGCAATGAAGTGTGTCCCAGTGGCAGATTTGGGAAAAACTGTGCAGGAATTTGTACCTGCACCAA CAACGGAACCTGTAACCCCATTGACAGATCTTGTCAGTGTTACCCCGGTTGGATTGGCAGTGACTGCTCT CAACCATGTCCACCTGCCCACTGGGGCCCAAACTGCATCCACACGTGCAACTGCCATAATGGAGCTTTCT GCAGCGCCTACGATGGGGAATGTAAATGCACTCCTGGCTGGACAGGGCTCTACTGCACTCAGAGATGTCC TCTAGGGTTTTATGGAAAAGATTGTGCACTGATATGCCAATGTCAAAACGGAGCTGACTGCGACCACATT TCTGGGCAGTGTACTTGCCGCACTGGATTCATGGGACGGCACTGTGAGCAGAAGTGCCCTTCAGGAACAT ATGGCTATGGCTGTCGCCAGATATGTGATTGTCTGAACAACTCCACCTGCGACCACATCACTGGGACCTG TTACTGCAGCCCCGGATGGAAGGGAGCGAGATGTGATCAAGCTGGTGTTATCATAGTTGGAAATCTGAAC AGCTTAAGCCGAACCAGTACTGCTCTCCCTGCTGATTCCTACCAGATCGGGGCCATTGCAGGCATCATCA TTCTTGTCCTAGTTGTTCTCTTCCTACTGGCATTGTTCATTATTTATAGACACAAGCAGAAGGGAAAGGA ATCAAGCATGCCAGCAGTTACCTACACCCCTGCTATGAGGGTCGTCAATGCAGATTATACCATTTCAGGA ACCCTTCCTCACAGCAATGGTGGAAACGCTAATAGCCACTACTTCACCAATCCCAGTTACCACACGCTCA CCCAGTGTGCCACATCCCCTCACGTCAACAACAGGGACAGGATGACTGTCACGAAGTCAAAAAACAATCA ACTGTTTGTGAATCTTAAAAATGTGAACCCTGGGAAGAGAGGCCCTGTGGGGGACTGCACTGGGACATTG CCGGCTGACTGGAAACATGGCGGCTACCTCAACGAGCTCGGTGCTTTTGGACTTGACAGAAGCTATATGG GAAAATCCTTAAAAGACCTGGGAAAGAATTCTGAATATAATTCAAGTAACTGCTCCCTAAGCAGTTCTGA GAACCCATATGCCACTATTAAAGACCCACCTGTACTTATCCCGAAAAGCTCAGAGTGTGGTTATGTGGAG ATGAAATCGCCGGCACGAAGAGATTCCCCATATGCAGAGATCAATAACTCAACTTCAGCCAACAGGAATG TCTATGAAGTTGAACCTACAGTGAGTGTTGTCCAAGGAGTATTCAGCAATAATGGGCGTCTCTCCCAGGA TCCATATGACCTCCCAAAGAACAGTCACATCCCTTGTCATTATGACCTGCTGCCAGTCCGAGACAGTTCA TCCTCCCCTAAGCAAGAGGACAGTGGTGGTAGCAGCAGCAACAGCAGCAGCAGCAGTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217883 representing NM_032446
Red=Cloning site Green=Tags(s) MVISLNSCLSFICLLLCHWIGTASPLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCT RHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACD GDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGEC RCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQE CQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERC EARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGAD CDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGT WGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCL PGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQ CVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGICTCTNNGTCNPIDRSCQCYPGWIGSDCS QPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHI SGQCTCRTGFMGRHCEQKCPSGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLN SLSRTSTALPADSYQIGAIAGIIILVLVVLFLLALFIIYRHKQKGKESSMPAVTYTPAMRVVNADYTISG TLPHSNGGNANSHYFTNPSYHTLTQCATSPHVNNRDRMTVTKSKNNQLFVNLKNVNPGKRGPVGDCTGTL PADWKHGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSNCSLSSSENPYATIKDPPVLIPKSSECGYVE MKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGRLSQDPYDLPKNSHIPCHYDLLPVRDSS SSPKQEDSGGSSSNSSSSSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032446 |
| ORF Size | 3420 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032446.3 |
| RefSeq Size | 7522 bp |
| RefSeq ORF | 3423 bp |
| Locus ID | 84466 |
| UniProt ID | Q96KG7 |
| Domains | EGF_Lam, EGF, EGF |
| Protein Families | Transmembrane |
| MW | 122 kDa |
| Gene Summary | This gene encodes a member of the multiple epidermal growth factor-like domains protein family. The encoded protein plays a role in cell adhesion, motility and proliferation, and is a critical mediator of apoptotic cell phagocytosis as well as amyloid-beta peptide uptake in the brain. Expression of this gene may be associated with schizophrenia, and mutations in this gene are a cause of early-onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD) as well as congenital myopathy with minicores. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Apr 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217883L1 | Lenti ORF clone of Human multiple EGF-like-domains 10 (MEGF10), Myc-DDK-tagged |
CNY 14,304.00 |
|
| RC217883L2 | Lenti ORF clone of Human multiple EGF-like-domains 10 (MEGF10), mGFP tagged |
CNY 7,600.00 |
|
| RC217883L3 | Lenti ORF clone of Human multiple EGF-like-domains 10 (MEGF10), Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC217883L4 | Lenti ORF clone of Human multiple EGF-like-domains 10 (MEGF10), mGFP tagged |
CNY 7,600.00 |
|
| RG217883 | MEGF10 (tGFP-tagged) - Human multiple EGF-like-domains 10 (MEGF10) |
CNY 13,504.00 |
|
| SC104847 | MEGF10 (untagged)-Human multiple EGF-like-domains 10 (MEGF10) |
CNY 11,920.00 |
