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SMPDL3B (NM_014474) Human Tagged ORF Clone
CAT＃: RC217688
SMPDL3B (Myc-DDK-tagged)-Human sphingomyelin phosphodiesterase, acid-like 3B (SMPDL3B), transcript variant 1
ORF Plasmid: tGFP
"NM_014474" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ASML3B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217688 representing NM_014474
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTGCTCGCCTGGCTGATTTTCCTGGCTAACTGGGGAGGTGCCAGGGCTGAACCAGGGAAGTTCT GGCACATCGCTGACCTGCACCTTGACCCTGACTACAAGGTATCCAAAGACCCCTTCCAGGTGTGCCCATC AGCTGGATCCCAGCCAGTGCCCGACGCAGGCCCCTGGGGTGACTACCTCTGTGATTCTCCCTGGGCCCTC ATCAACTCCTCCATCTATGCCATGAAGGAGATTGAGCCAGAGCCAGACTTCATTCTCTGGACTGGTGATG ACACGCCTCATGTGCCCGATGAGAAACTGGGAGAGGCAGCTGTACTGGAAATTGTGGAACGCCTGACCAA GCTCATCAGAGAGGTCTTTCCAGATACTAAAGTCTATGCTGCTTTGGGAAATCATGATTTTCACCCCAAA AACCAGTTCCCAGCTGGAAGTAACAACATCTACAATCAGATAGCAGAACTATGGAAACCCTGGCTTAGTA ATGAGTCCATCGCTCTCTTCAAAAAAGGTGCCTTCTACTGTGAGAAGCTGCCGGGTCCCAGCGGGGCTGG GCGAATTGTGGTCCTCAACACCAATCTGTACTATACCAGCAATGCGCTGACAGCAGACATGGCGGACCCT GGCCAGCAGTTCCAGTGGCTGGAAGATGTGCTGACCGATGCATCCAAAGCTGGGGACATGGTGTACATTG TCGGCCACGTGCCCCCGGGGTTCTTTGAGAAGACGCAAAACAAGGCATGGTTCCGGGAGGGCTTCAATGA AAAATACCTGAAGGTGGTCCGGAAGCATCATCGCGTCATAGCAGGGCAGTTCTTCGGGCACCACCACACC GACAGCTTTCGGATGCTCTATGATGATGCAGGTGTCCCCATAAGCGCCATGTTCATCACACCTGGAGTCA CCCCATGGAAAACCACATTACCTGGAGTGGTCAATGGGGCCAACAATCCAGCCATCCGGGTGTTCGAATA TGACCGAGCCACACTGAGCCTGAAGGACATGGTGACCTACTTCATGAACCTGAGCCAGGCGAATGCTCAG GGGACGCCGCGCTGGGAGCTCGAGTACCAGCTGACCGAGGCCTATGGGGTGCCGGACGCCAGCGCCCACT CCATGCACACAGTGCTGGACCGCATCGCTGGCGACCAGAGCACACTGCAGCGCTACTACGTCTATAACTC AGTCAGCTACTCTGCTGGGGTCTGCGACGAGGCCTGCAGCATGCAGCACGTGTGTGCCATGCGCCAGGTG GACATTGACGCTTACACCACCTGTCTGTATGCCTCTGGCACCACGCCCGTGCCCCAGCTCCCGCTGCTGC TGATGGCCCTGCTGGGCCTGTGCACGCTCGTGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217688 representing NM_014474
Red=Cloning site Green=Tags(s) MRLLAWLIFLANWGGARAEPGKFWHIADLHLDPDYKVSKDPFQVCPSAGSQPVPDAGPWGDYLCDSPWAL INSSIYAMKEIEPEPDFILWTGDDTPHVPDEKLGEAAVLEIVERLTKLIREVFPDTKVYAALGNHDFHPK NQFPAGSNNIYNQIAELWKPWLSNESIALFKKGAFYCEKLPGPSGAGRIVVLNTNLYYTSNALTADMADP GQQFQWLEDVLTDASKAGDMVYIVGHVPPGFFEKTQNKAWFREGFNEKYLKVVRKHHRVIAGQFFGHHHT DSFRMLYDDAGVPISAMFITPGVTPWKTTLPGVVNGANNPAIRVFEYDRATLSLKDMVTYFMNLSQANAQ GTPRWELEYQLTEAYGVPDASAHSMHTVLDRIAGDQSTLQRYYVYNSVSYSAGVCDEACSMQHVCAMRQV DIDAYTTCLYASGTTPVPQLPLLLMALLGLCTLVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014474 |
| ORF Size | 1365 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014474.4 |
| RefSeq Size | 1890 bp |
| RefSeq ORF | 1368 bp |
| Locus ID | 27293 |
| UniProt ID | Q92485 |
| Domains | Metallophos |
| Protein Families | Secreted Protein |
| MW | 50.6 kDa |
| Gene Summary | Lipid-modulating phosphodiesterase (PubMed:26095358). Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling (By similarity). Has in vitro phosphodiesterase activity, but the physiological substrate is unknown (PubMed:26095358). Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Regulation of the amount of ceramide-1-phosphate synthesized in differentiated human podocytes
,Mallela, SK;Mitrofanova, A;Merscher, S;Fornoni, A;,
Biochim Biophys Acta Mol Cell Biol Lipids
,PubMed ID 31487557
[SMPDL3B]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217688L3 | Lenti ORF clone of Human sphingomyelin phosphodiesterase, acid-like 3B (SMPDL3B), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217688L4 | Lenti ORF clone of Human sphingomyelin phosphodiesterase, acid-like 3B (SMPDL3B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG217688 | SMPDL3B (tGFP-tagged) - Human sphingomyelin phosphodiesterase, acid-like 3B (SMPDL3B), transcript variant 1 |
CNY 4,370.00 |
|
| SC108594 | SMPDL3B (untagged)-Human sphingomyelin phosphodiesterase, acid-like 3B (SMPDL3B), transcript variant 1 |
CNY 3,656.00 |
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