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LLGL1 (NM_004140) Human Tagged ORF Clone
CAT＃: RC217078
LLGL1 (Myc-DDK-tagged)-Human lethal giant larvae homolog 1 (Drosophila) (LLGL1)
ORF Plasmid: tGFP
"NM_004140" in other vectors (4)
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CNY 7,408.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DLG4; HUGL; HUGL-1; HUGL1; Lgl1; LLGL; Mgl1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217078 representing NM_004140
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAAGTTTCGGTTCCGGCGGCAGGGCGCCGACCCGCAGCGCGAGAAGCTCAAGCAGGAGCTTTTCG CCTTCAACAAGACTGTGGAGCATGGCTTCCCCAATCAGCCCAGCGCCCTGGCCTTCGACCCGGAACTTCG CATCATGGCCATCGGCACCAGGTCTGGGGCTGTCAAGATCTATGGTGCACCTGGCGTGGAGTTCACAGGC CTGCACCGGGATGCAGCCACTGTCACACAGATGCACTTCTTGACCGGCCAGGGCCGCCTCCTGTCCCTGC TTGATGACAGCAGTCTGCATCTCTGGGAGATTGTCCACCATAATGGCTGTGCCCACCTGGAAGAAGCACT CAGTTTCCAGCTGCCCAGCCGGCCCGGCTTTGATGGTGCCAGTGCTCCGCTCAGCCTTACCCGAGTCACA GTGGTCCTGCTGGTGGCTGCCGGCGACATAGCAGCCCTGGGCACTGAGGGCAGCAGTGTCTTCTTCCTGG ATGTTACCACCCTGACCCTGCTCGAGGGGCAGACGCTTGCCCCAGGCGAGGTTCTGCGCAGCGTGCCAGA CGACTACCGCTGTGGGAAGGCACTGGGCCCCGTGGAGTCACTCCAGGGACACCTGCGGGACCCCACAAAG ATTCTCATTGGCTACAGCCGGGGCCTGCTGGTCATCTGGAACCAGGCCTCGCAGTGTGTGGACCACATCT TCCTGGGGAACCAGCAGCTGGAGAGCCTATGCTGGGGGCGTGATAGCAGCACTGTGGTCAGCTCACACAG CGATGGCAGCTATGCTGTCTGGTCTGTGGATGCCGGCAGCTTCCCAACGCTGCAGCCCACGGTAGCCACC ACACCTTACGGCCCCTTTCCCTGCAAGGCCATTAACAAGATTCTGTGGCGGAACTGTGAATCTGGGGGCC ACTTTATCATCTTCAGCGGTGGCATGCCCCGTGCCAGCTATGGTGACCGCCACTGTGTAAGTGTGCTTCG AGCCGAGACATTGGTGACGCTGGACTTCACTTCCCGCATCATCGACTTCTTCACAGTGCACAGCACACGG CCCGAGGATGAATTTGATGACCCCCAGGCCCTGGCTGTGCTGCTGGAAGAGGAGCTGGTGGTGCTGGACC TGCAGACTCCTGGCTGGCCAGCTGTGCCTGCCCCATACCTGGCCCCGCTGCACTCCTCTGCAATCACTTG CTCGGCCCACGTGGCCAGTGTCCCCGCCAAGCTGTGGGCCCGCATTGTGAGCGCTGGCGAGCAGCAGAGC CCCCAGCCTGTCTCCAGTGCCTTGAGCTGGCCCATCACTGGGGGCCGAAACCTGGCCCAGGAGCCGTCAC AGCGAGGGCTGCTGCTGACGGGCCATGAGGACGGCACCGTGAGGTTCTGGGATGCCTCGGGTGTGGCGCT GCGGCCGCTCTATAAGCTGAGCACAGCTGGCCTCTTCCAGACAGACTGTGAGCACGCTGACAGCCTGGCC CAGGCTGCCGAGGACGACTGGCCACCCTTCCGCAAGGTGGGCTGCTTCGATCCCTACAGTGACGATCCCC GGCTTGGCGTGCAGAAGGTTGCTCTCTGCAAGTATACAGCCCAGATGGTGGTGGCTGGCACTGCAGGCCA GGTGCTGGTACTGGAGCTTAGTGATGTGCCGGTGGAGCAGGCGGTCAGCGTGGCCATCATAGACCTCCTC CAGGACCGCGAGGGCTTCACATGGAAGGGCCACGAGCGGCTGAGCCCACGCACGGGGCCGCTGCCCTGGC CTGCTGGCTTCCAGCCCCGTGTCCTGGTGCAGTGCCTGCCGCCAGCTGCTGTAACCGCTGTCACACTCCA CACCGAGTGGAGCCTCGTGGCTTTTGGCACCAGTCATGGCTTTGGCCTCTTCGACTACCAGCGCAAGAGC CCTGTGCTGGCCAGGTGCACTCTTCACCCCAATGACTCCCTGGCCATGGAGGGTCCGCTCTCCCGGGTGA AGTCTCTCAAGAAGTCACTGCGCCAGTCTTTCCGGCGCATTCGCAAGAGTCGTGTCTCTGGCAAGAAGCG GGCTGCTAATGCCAGCAGCAAGTTGCAGGAAGCCAATGCACAGCTGGCTGAGCAGGCCTGCCCCCACGAC GTGGAGATGACGCCCGTGCAGCGCCGCATTGAGCCCCGCTCTGCCGATGACTCCTTGTCGGGTGTCGTGC GTTGCCTATACTTTGCCGACACATTCCTTCGAGATGGGGCCCACCACGGGCCCACCATGTGGGCTGGCAC CAACTCAGGCTCTGTGTTCGCCTATGCACTGGAGGTGCCGGCAGCAGCAGTGGGTGGTGAGAAGCGGCCT GAGCAAGCGGTGGAGGCCGTGCTGGGCAAGGAGGTGCAGCTGATGCACCGGGCGCCTGTGGTGGCCATTG CCGTGTTGGACGGGCGTGGCCGCCCACTGCCCGAGCCCTACGAGGCCTCACGGGACCTGGCGCAGGCACC TGACATGCAGGGTGGTCACGCTGTGCTCATCGCATCTGAGGAGCAGTTCAAGGTGTTCACACTGCCCAAG GTGAGCGCGAAGACCAAGTTCAAGCTGACGGCCCATGAGGGCTGTCGTGTGCGCAAGGTGGCACTGGCCA CGTTTGCCAGTGTGGCCTGCGAGGACTATGCTGAGACCTGCCTGGCCTGCCTCACCAACCTGGGTGACGT CCACGTCTTCTCGGTGCCTGGCCTGCGGCCCCAGGTGCACTATTCCTGCATCCGGAAGGAGGACATCAGC GGCATCGCTTCGTGCGTCTTTACGCGCCATGGCCAGGGCTTTTACCTGATATCCCCATCAGAATTTGAAC GCTTCTCCCTAAGTGCCCGGAACATCACAGAGCCGCTCTGCTCTCTGGACATTAACTGGCCCCGCGATGC CACCCAGGCCAGTTACAGGATCCGAGAGTCACCCAAGCTGAGCCAGGCTAACGGGACCCCAAGCATCCTG CTGGCCCCACAGAGCCTTGATGGAAGCCCTGATCCAGCCCACAGCATGGGACCTGACACCCCGGAGCCAC CCGAGGCTGCACTCTCACCCATGTCCATCGACTCAGCCACCAGTGCTGACACCACGCTGGACACGACAGG GGACGTCACAGTGGAAGATGTGAAGGATTTCCTGGGCTCCTCTGAGGAGTCAGAGAAGAACCTGAGGAAC CTGGCAGAAGACGAGGCCCACGCCTGTGCCATCCTGATCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217078 representing NM_004140
Red=Cloning site Green=Tags(s) MMKFRFRRQGADPQREKLKQELFAFNKTVEHGFPNQPSALAFDPELRIMAIGTRSGAVKIYGAPGVEFTG LHRDAATVTQMHFLTGQGRLLSLLDDSSLHLWEIVHHNGCAHLEEALSFQLPSRPGFDGASAPLSLTRVT VVLLVAAGDIAALGTEGSSVFFLDVTTLTLLEGQTLAPGEVLRSVPDDYRCGKALGPVESLQGHLRDPTK ILIGYSRGLLVIWNQASQCVDHIFLGNQQLESLCWGRDSSTVVSSHSDGSYAVWSVDAGSFPTLQPTVAT TPYGPFPCKAINKILWRNCESGGHFIIFSGGMPRASYGDRHCVSVLRAETLVTLDFTSRIIDFFTVHSTR PEDEFDDPQALAVLLEEELVVLDLQTPGWPAVPAPYLAPLHSSAITCSAHVASVPAKLWARIVSAGEQQS PQPVSSALSWPITGGRNLAQEPSQRGLLLTGHEDGTVRFWDASGVALRPLYKLSTAGLFQTDCEHADSLA QAAEDDWPPFRKVGCFDPYSDDPRLGVQKVALCKYTAQMVVAGTAGQVLVLELSDVPVEQAVSVAIIDLL QDREGFTWKGHERLSPRTGPLPWPAGFQPRVLVQCLPPAAVTAVTLHTEWSLVAFGTSHGFGLFDYQRKS PVLARCTLHPNDSLAMEGPLSRVKSLKKSLRQSFRRIRKSRVSGKKRAANASSKLQEANAQLAEQACPHD VEMTPVQRRIEPRSADDSLSGVVRCLYFADTFLRDGAHHGPTMWAGTNSGSVFAYALEVPAAAVGGEKRP EQAVEAVLGKEVQLMHRAPVVAIAVLDGRGRPLPEPYEASRDLAQAPDMQGGHAVLIASEEQFKVFTLPK VSAKTKFKLTAHEGCRVRKVALATFASVACEDYAETCLACLTNLGDVHVFSVPGLRPQVHYSCIRKEDIS GIASCVFTRHGQGFYLISPSEFERFSLSARNITEPLCSLDINWPRDATQASYRIRESPKLSQANGTPSIL LAPQSLDGSPDPAHSMGPDTPEPPEAALSPMSIDSATSADTTLDTTGDVTVEDVKDFLGSSEESEKNLRN LAEDEAHACAILIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004140 |
| ORF Size | 3192 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004140.3, NP_004131.3 |
| RefSeq Size | 4225 bp |
| RefSeq ORF | 3195 bp |
| Locus ID | 3996 |
| UniProt ID | Q15334 |
| Protein Families | Druggable Genome |
| Protein Pathways | Tight junction |
| MW | 115.4 kDa |
| Gene Summary | This gene encodes a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217078L3 | Lenti-ORF clone of LLGL1 (Myc-DDK-tagged)-Human lethal giant larvae homolog 1 (Drosophila) (LLGL1) |
CNY 12,540.00 |
|
| RC217078L4 | Lenti-ORF clone of LLGL1 (mGFP-tagged)-Human lethal giant larvae homolog 1 (Drosophila) (LLGL1) |
CNY 12,540.00 |
|
| RG217078 | LLGL1 (tGFP-tagged) - Human lethal giant larvae homolog 1 (Drosophila) (LLGL1) |
CNY 9,008.00 |
|
| SC303440 | LLGL1 (untagged)-Human lethal giant larvae homolog 1 (Drosophila) (LLGL1) |
CNY 10,450.00 |
