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TRPM2 (NM_003307) Human Tagged ORF Clone
CAT＃: RC216220
TRPM2 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant 1
ORF Plasmid: tGFP
"NM_003307" in other vectors (4)
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CNY 10,288.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EREG1; KNP3; LTrpC-2; LTRPC2; NUDT9H; NUDT9L1; TRPC7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216220 representing NM_003307
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCTCAGCCCTGAGGAAAGCTGGCTCGGAGCAGGAGGAGGGCTTTGAGGGGCTGCCCAGAAGGG TCACTGACCTGGGGATGGTCTCCAATCTCCGGCGCAGCAACAGCAGCCTCTTCAAGAGCTGGAGGCTACA GTGCCCCTTCGGCAACAATGACAAGCAAGAAAGCCTCAGTTCGTGGATTCCTGAAAACATCAAGAAGAAA GAATGCGTGTATTTTGTGGAAAGTTCCAAACTGTCTGATGCTGGGAAGGTGGTGTGTCAGTGTGGCTACA CGCATGAGCAGCACTTGGAGGAGGCTACCAAGCCCCACACCTTCCAGGGCACACAGTGGGACCCAAAGAA ACATGTCCAGGAGATGCCAACCGATGCCTTTGGCGACATCGTCTTCACGGGCCTGAGCCAGAAGGTGAAA AAGTACGTCCGAGTCTCCCAGGACACGCCCTCCAGCGTGATCTACCACCTCATGACCCAGCACTGGGGGC TGGACGTCCCCAATCTCTTGATCTCGGTGACCGGGGGGGCCAAGAACTTCAACATGAAGCCGCGGCTGAA GAGCATTTTCCGCAGAGGCCTGGTCAAGGTGGCTCAGACCACAGGGGCCTGGATCATCACAGGGGGGTCC CACACCGGCGTCATGAAGCAGGTAGGCGAGGCGGTGCGGGACTTCAGCCTGAGCAGCAGCTACAAGGAAG GCGAGCTCATCACCATCGGAGTCGCCACCTGGGGCACTGTCCACCGCCGCGAGGGCCTGATCCATCCCAC GGGCAGCTTCCCCGCCGAGTACATACTGGATGAGGATGGCCAAGGGAACCTGACCTGCCTAGACAGCAAC CACTCTCACTTCATCCTCGTGGACGACGGGACCCACGGCCAGTACGGGGTGGAGATTCCTCTGAGGACCA GGCTGGAGAAGTTCATATCGGAGCAGACCAAGGAAAGAGGAGGTGTGGCCATCAAGATCCCCATCGTGTG CGTGGTGCTGGAGGGCGGCCCGGGCACGTTGCACACCATCGACAACGCCACCACCAACGGCACCCCCTGT GTGGTTGTGGAGGGCTCGGGCCGCGTGGCCGACGTCATTGCCCAGGTGGCCAACCTGCCTGTCTCGGACA TCACTATCTCCCTGATCCAGCAGAAACTGAGCGTGTTCTTCCAGGAGATGTTTGAGACCTTCACGGAAAG CAGGATTGTCGAGTGGACCAAAAAGATCCAAGATATCGTCCGGAGGCGGCAGCTGCTGACTGTCTTCCGG GAAGGCAAGGATGGTCAGCAGGACGTGGATGTGGCCATCTTGCAGGCCTTGCTGAAAGCCTCACGGAGCC AAGACCACTTTGGCCACGAGAACTGGGACCACCAGCTGAAACTGGCAGTGGCATGGAATCGCGTGGACAT TGCCCGCAGTGAGATCTTCATGGATGAGTGGCAGTGGAAGCCTTCAGATCTGCACCCCACGATGACAGCT GCACTCATCTCCAACAAGCCTGAGTTTGTGAAGCTCTTCCTGGAGAACGGGGTGCAGCTGAAGGAGTTTG TCACCTGGGACACCTTGCTCTACCTGTACGAGAACCTGGACCCCTCCTGCCTGTTCCACAGCAAGCTGCA GAAGGTGCTGGTGGAGGATCCCGAGCGCCCGGCTTGCGCGCCCGCGGCGCCCCGCCTGCAGATGCACCAC GTGGCCCAGGTGCTGCGGGAGCTGCTGGGGGACTTCACGCAGCCGCTTTATCCCCGGCCCCGGCACAACG ACCGGCTGCGGCTCCTGCTGCCCGTTCCCCACGTCAAGCTCAACGTGCAGGGAGTGAGCCTCCGGTCCCT CTACAAGCGTTCCTCAGGCCATGTGACCTTCACCATGGACCCCATCCGTGACCTTCTCATTTGGGCCATT GTCCAGAACCGTCGGGAGCTGGCAGGAATCATCTGGGCTCAGAGCCAGGACTGCATCGCAGCGGCCTTGG CCTGCAGCAAGATCCTGAAGGAACTGTCCAAGGAGGAGGAGGACACGGACAGCTCGGAGGAGATGCTGGC GCTGGCGGAGGAGTATGAGCACAGAGCCATCGGGGTCTTCACCGAGTGCTACCGGAAGGACGAAGAGAGA GCCCAGAAACTGCTCACCCGCGTGTCCGAGGCCTGGGGGAAGACCACCTGCCTGCAGCTCGCCCTGGAGG CCAAGGACATGAAGTTTGTGTCTCACGGGGGCATCCAGGCCTTCCTGACCAAGGTGTGGTGGGGCCAGCT CTCCGTGGACAATGGGCTGTGGCGTGTGACCCTGTGCATGCTGGCCTTCCCGCTGCTCCTCACCGGCCTC ATCTCCTTCAGGGAGAAGAGGCTGCAGGATGTGGGCACCCCCGCGGCCCGCGCCCGTGCCTTCTTCACCG CACCCGTGGTGGTCTTCCACCTGAACATCCTCTCCTACTTCGCCTTCCTCTGCCTGTTCGCCTACGTGCT CATGGTGGACTTCCAGCCTGTGCCCTCCTGGTGCGAGTGTGCCATCTACCTCTGGCTCTTCTCCTTGGTG TGCGAGGAGATGCGGCAGCTCTTCTATGACCCTGACGAGTGCGGGCTGATGAAGAAGGCAGCCTTGTACT TCAGTGACTTCTGGAATAAGCTGGACGTCGGCGCAATCTTGCTCTTCGTGGCAGGGCTGACCTGCAGGCT CATCCCGGCGACGCTGTACCCCGGGCGCGTCATCCTCTCTCTGGACTTCATCCTGTTCTGCCTCCGGCTC ATGCACATTTTTACCATCAGTAAGACGCTGGGGCCCAAGATCATCATTGTGAAGCGGATGATGAAGGACG TCTTCTTCTTCCTCTTCCTGCTGGCTGTGTGGGTGGTGTCCTTCGGGGTGGCCAAGCAGGCCATCCTCAT CCACAACGAGCGCCGGGTGGACTGGCTGTTCCGAGGGGCCGTCTACCACTCCTACCTCACCATCTTCGGG CAGATCCCGGGCTACATCGACGGTGTGAACTTCAACCCGGAGCACTGCAGCCCCAATGGCACCGACCCCT ACAAGCCTAAGTGCCCCGAGAGCGACGCGACGCAGCAGAGGCCGGCCTTCCCTGAGTGGCTGACGGTCCT CCTACTCTGCCTCTACCTGCTCTTCACCAACATCCTGCTGCTCAACCTCCTCATCGCCATGTTCAACTAC ACCTTCCAGCAGGTGCAGGAGCACACGGACCAGATTTGGAAGTTCCAGCGCCATGACCTGATCGAGGAGT ACCACGGCCGCCCCGCCGCGCCGCCCCCCTTCATCCTCCTCAGCCACCTGCAGCTCTTCATCAAGAGGGT GGTCCTGAAGACTCCGGCCAAGAGGCACAAGCAGCTCAAGAACAAGCTGGAGAAGAACGAGGAGGCGGCC CTGCTATCCTGGGAGATCTACCTGAAGGAGAACTACCTCCAGAACCGACAGTTCCAGCAAAAGCAGCGGC CCGAGCAGAAGATCGAGGACATCAGCAATAAGGTTGACGCCATGGTGGACCTGCTGGACCTGGACCCACT GAAGAGGTCGGGCTCCATGGAGCAGAGGTTGGCCTCCCTGGAGGAGCAGGTGGCCCAGACAGCCCGAGCC CTGCACTGGATCGTGAGGACGCTGCGGGCCAGCGGCTTCAGCTCGGAGGCGGACGTCCCCACTCTGGCCT CCCAGAAGGCCGCGGAGGAGCCGGATGCTGAGCCGGGAGGCAGGAAGAAGACGGAGGAGCCGGGCGACAG CTACCACGTGAATGCCCGGCACCTCCTCTACCCCAACTGCCCTGTCACGCGCTTCCCCGTGCCCAACGAG AAGGTGCCCTGGGAGACGGAGTTCCTGATCTATGACCCACCCTTTTACACGGCAGAGAGGAAGGACGCGG CCGCCATGGACCCCATGGGAGACACCCTGGAGCCACTGTCCACGATCCAGTACAACGTGGTGGATGGCCT GAGGGACCGCCGGAGCTTCCACGGGCCGTACACAGTGCAGGCCGGGTTGCCCCTGAACCCCATGGGCCGC ACAGGACTGCGTGGGCGCGGGAGCCTCAGCTGCTTCGGACCCAACCACACGCTGTACCCCATGGTCACGC GGTGGAGGCGGAACGAGGATGGAGCCATCTGCAGGAAGAGCATAAAGAAGATGCTGGAAGTGCTGGTGGT GAAGCTCCCTCTCTCCGAGCACTGGGCCCTGCCTGGGGGCTCCCGGGAGCCAGGGGAGATGCTACCTCGG AAGCTGAAGCGGATCCTCCGGCAGGAGCACTGGCCGTCTTTTGAAAACTTGCTGAAGTGCGGCATGGAGG TGTACAAAGGCTACATGGATGACCCGAGGAACACGGACAATGCCTGGATCGAGACGGTGGCCGTCAGCGT CCACTTCCAGGACCAGAATGACGTGGAGCTGAACAGGCTGAACTCTAACCTGCACGCCTGCGACTCGGGG GCCTCCATCCGATGGCAGGTGGTGGACAGGCGCATCCCACTCTATGCGAACCACAAGACCCTCCTCCAGA AGGCAGCCGCTGAGTTCGGGGCTCACTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216220 representing NM_003307
Red=Cloning site Green=Tags(s) MEPSALRKAGSEQEEGFEGLPRRVTDLGMVSNLRRSNSSLFKSWRLQCPFGNNDKQESLSSWIPENIKKK ECVYFVESSKLSDAGKVVCQCGYTHEQHLEEATKPHTFQGTQWDPKKHVQEMPTDAFGDIVFTGLSQKVK KYVRVSQDTPSSVIYHLMTQHWGLDVPNLLISVTGGAKNFNMKPRLKSIFRRGLVKVAQTTGAWIITGGS HTGVMKQVGEAVRDFSLSSSYKEGELITIGVATWGTVHRREGLIHPTGSFPAEYILDEDGQGNLTCLDSN HSHFILVDDGTHGQYGVEIPLRTRLEKFISEQTKERGGVAIKIPIVCVVLEGGPGTLHTIDNATTNGTPC VVVEGSGRVADVIAQVANLPVSDITISLIQQKLSVFFQEMFETFTESRIVEWTKKIQDIVRRRQLLTVFR EGKDGQQDVDVAILQALLKASRSQDHFGHENWDHQLKLAVAWNRVDIARSEIFMDEWQWKPSDLHPTMTA ALISNKPEFVKLFLENGVQLKEFVTWDTLLYLYENLDPSCLFHSKLQKVLVEDPERPACAPAAPRLQMHH VAQVLRELLGDFTQPLYPRPRHNDRLRLLLPVPHVKLNVQGVSLRSLYKRSSGHVTFTMDPIRDLLIWAI VQNRRELAGIIWAQSQDCIAAALACSKILKELSKEEEDTDSSEEMLALAEEYEHRAIGVFTECYRKDEER AQKLLTRVSEAWGKTTCLQLALEAKDMKFVSHGGIQAFLTKVWWGQLSVDNGLWRVTLCMLAFPLLLTGL ISFREKRLQDVGTPAARARAFFTAPVVVFHLNILSYFAFLCLFAYVLMVDFQPVPSWCECAIYLWLFSLV CEEMRQLFYDPDECGLMKKAALYFSDFWNKLDVGAILLFVAGLTCRLIPATLYPGRVILSLDFILFCLRL MHIFTISKTLGPKIIIVKRMMKDVFFFLFLLAVWVVSFGVAKQAILIHNERRVDWLFRGAVYHSYLTIFG QIPGYIDGVNFNPEHCSPNGTDPYKPKCPESDATQQRPAFPEWLTVLLLCLYLLFTNILLLNLLIAMFNY TFQQVQEHTDQIWKFQRHDLIEEYHGRPAAPPPFILLSHLQLFIKRVVLKTPAKRHKQLKNKLEKNEEAA LLSWEIYLKENYLQNRQFQQKQRPEQKIEDISNKVDAMVDLLDLDPLKRSGSMEQRLASLEEQVAQTARA LHWIVRTLRASGFSSEADVPTLASQKAAEEPDAEPGGRKKTEEPGDSYHVNARHLLYPNCPVTRFPVPNE KVPWETEFLIYDPPFYTAERKDAAAMDPMGDTLEPLSTIQYNVVDGLRDRRSFHGPYTVQAGLPLNPMGR TGLRGRGSLSCFGPNHTLYPMVTRWRRNEDGAICRKSIKKMLEVLVVKLPLSEHWALPGGSREPGEMLPR KLKRILRQEHWPSFENLLKCGMEVYKGYMDDPRNTDNAWIETVAVSVHFQDQNDVELNRLNSNLHACDSG ASIRWQVVDRRIPLYANHKTLLQKAAAEFGAHY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003307 |
| ORF Size | 4509 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003307.3, NP_003298.1 |
| RefSeq Size | 5876 bp |
| RefSeq ORF | 4512 bp |
| Locus ID | 7226 |
| UniProt ID | O94759 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| MW | 171.7 kDa |
| Gene Summary | The protein encoded by this gene forms a tetrameric cation channel that is permeable to calcium, sodium, and potassium and is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. Additional transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216220L3 | Lenti-ORF clone of TRPM2 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant 1 |
CNY 16,910.00 |
|
| RC216220L4 | Lenti-ORF clone of TRPM2 (mGFP-tagged)-Human transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant 1 |
CNY 12,688.00 |
|
| RG216220 | TRPM2 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant 1 |
CNY 11,888.00 |
|
| SC303292 | TRPM2 (untagged)-Human transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant 1 |
CNY 10,288.00 |
