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HIF3 alpha (HIF3A) (NM_022462) Human Tagged ORF Clone
CAT＃: RC215953
- TrueORF®
HIF3A (Myc-DDK-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 2
ORF Plasmid: tGFP
"NM_022462" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bHLHe17; HIF-3A; HIF3-alpha-1; IPAS; MOP7; PASD7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215953 representing NM_022462
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCCCGCAGCCGGCGCAGCCAGGAGACCGAGGTGCTGTACCAGCTGGCTCACACGCTGCCCTTCGC CCGCGGCGTCAGCGCCCACCTGGACAAGGCCTCTATCATGCGCCTCACCATCAGCTACCTGCGCATGCAC CGCCTCTGCGCCGCAGCTGGAGCTCATTGGACACAGCATCTTTGATTTCATCCACCCCTGTGACCAAGAG GAGCTTCAGGACGCCCTGACCCCCCAGCAGACCCTGTCCAGGAGGAAGGTGGAGGCCCCCACGGAGCGGT GCTTCTCCTTGCGCATGAAGAGTACACTCACCAGCCGCGGGCGCACCCTCAACCTCAAGGCGGCCACCTG GAAGGTGCTGAACTGCTCTGGACATATGAGGGCCTACAAGCCACCTGCGCAGACTTCTCCAGCTGGGAGC CCTGACTCAGAGCCCCCGCTGCAGTGCCTGGTGCTCATCTGCGAAGCCATCCCCCACCCAGGCAGCCTGG AGCCCCCACTGGGCCGAGGGGCCTTCCTCAGCCGCCACAGCCTGGACATGAAGTTCACCTACTGTGACGA CAGGATTGCAGAAGTGGCTGGCTATAGTCCCGATGACCTGATCGGCTGTTCCGCCTACGAGTACATCCAC GCGCTGGACTCCGATGCGGTCAGCAAGAGCATCCACACCTTGCTGAGCAAGGGCCAGGCAGTAACAGGGC AGTATCGCTTCCTGGCCCGGAGTGGTGGCTACCTGTGGACCCAGACCCAGGCCACAGTGGTGTCAGGGGG ACGGGGCCCCCAGTCGGAGAGTATCGTCTGTGTCCATTTTTTAATCAGCCAGGTGGAAGAGACCGGAGTG GTGCTGTCCCTGGAGCAAACGGAGCAACACTCTCGCAGACCCATTCAGCGGGGCGCCCCCTCTCAGAAGG ACACCCCTAACCCTGGGGACAGCCTTGACACCCCTGGCCCCCGGATCCTTGCCTTCCTGCACCCGCCTTC CCTGAGCGAGGCTGCCCTGGCCGCTGACCCCCGCCGTTTCTGCAGCCCTGACCTCCGTCGCCTCCTGGGA CCCATCCTGGATGGGGCTTCAGTAGCAGCCACTCCCAGCACCCCGCTGGCCACACGGCACCCCCAAAGTC CTCTTTCGGCTGATCTCCCAGATGAACTACCTGTGGGCACCGAGAATGTGCACAGACTCTTCACCTCCGG GAAAGACACTGAGGCAGTGGAGACAGATTTAGATATAGCTCAGGATGCTGATGCTCTGGATTTGGAGATG CTGGCCCCCTACATCTCCATGGATGATGACTTCCAGCTCAACGCCAGCGAGCAGCTACCCAGGGCCTACC ACAGACCTCTGGGGGCTGTCCCCCGGCCCCGTGCTCGGAGCTTCCATGGCCTGTCACCTCCAGCCCTTGA GCCCTCCCTGCTACCCCGCTGGGGGAGTGACCCCCGGCTGAGCTGCTCCAGCCCTTCCAGAGGGGACCCC TCAGCATCCTCTCCCATGGCTGGGGCTCGGAAGAGGACCCTGGCCCAGAGCTCAGAGGACGAGGACGAGG GAGTGGAGCTGCTGGGAGTGAGACCTCCCAAAAGGTCCCCCAGCCCAGAACACGAAAACTTTCTGCTCTT TCCTCTCAGCCTGAGTTTCCTTCTGACAGGAGGACCAGCCCCAGGGAGCCTGCAGGACCCCAGCACCCCA CTCCTGAACCTGAATGAGCCCCTGGGCCTGGGCCCCTCACTGCTCTCTCCGTACTCAGACGAGGACACTA CCCAGCCCGGGGGCCCCTTCCAGCCAAGGGCAGGCTCAGCCCAGGCTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215953 representing NM_022462
Red=Cloning site Green=Tags(s) MRPAAGAARRPRCCTSWLTRCPSPAASAPTWTRPLSCASPSATCACTASAPQLELIGHSIFDFIHPCDQE ELQDALTPQQTLSRRKVEAPTERCFSLRMKSTLTSRGRTLNLKAATWKVLNCSGHMRAYKPPAQTSPAGS PDSEPPLQCLVLICEAIPHPGSLEPPLGRGAFLSRHSLDMKFTYCDDRIAEVAGYSPDDLIGCSAYEYIH ALDSDAVSKSIHTLLSKGQAVTGQYRFLARSGGYLWTQTQATVVSGGRGPQSESIVCVHFLISQVEETGV VLSLEQTEQHSRRPIQRGAPSQKDTPNPGDSLDTPGPRILAFLHPPSLSEAALAADPRRFCSPDLRRLLG PILDGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDLDIAQDADALDLEM LAPYISMDDDFQLNASEQLPRAYHRPLGAVPRPRARSFHGLSPPALEPSLLPRWGSDPRLSCSSPSRGDP SASSPMAGARKRTLAQSSEDEDEGVELLGVRPPKRSPSPEHENFLLFPLSLSFLLTGGPAPGSLQDPSTP LLNLNEPLGLGPSLLSPYSDEDTTQPGGPFQPRAGSAQAD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022462 |
| ORF Size | 1800 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022462.4 |
| RefSeq Size | 2626 bp |
| RefSeq ORF | 1803 bp |
| Locus ID | 64344 |
| UniProt ID | Q9Y2N7 |
| Domains | PAS |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 66 kDa |
| Gene Summary | The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215953L3 | Lenti-ORF clone of HIF3A (Myc-DDK-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 2 |
CNY 5,890.00 |
|
| RC215953L4 | Lenti-ORF clone of HIF3A (mGFP-tagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 2 |
CNY 5,890.00 |
|
| RG215953 | HIF3A (tGFP-tagged) - Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 2 |
CNY 4,370.00 |
|
| SC310198 | HIF3A (untagged)-Human hypoxia inducible factor 3, alpha subunit (HIF3A), transcript variant 2 |
CNY 7,220.00 |
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