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CDC42 binding protein kinase alpha (CDC42BPA) (NM_003607) Human Tagged ORF Clone
CAT＃: RC214083
- TrueORF®
CDC42BPA (Myc-DDK-tagged)-Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant B
ORF Plasmid: tGFP
"NM_003607" in other vectors (3)
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CNY 3,656.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MRCK; MRCKA; PK428 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214083 representing NM_003607
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGAGAAGTGCGTTTGAGGCAGTTGGAGCAGTTTATTTTGGACGGGCCCGCTCAGACCAATGGGC AGTGCTTCAGTGTGGAGACGTTACTGGATATACTCATCTGCCTTTATGATGAATGCAATAATTCTCCATT GAGAAGAGAGAAGAACATTCTCGAATACCTAGAATGGGCTAAACCATTTACTTCTAAAGTGAAACAAATG CGATTACATAGAGAAGACTTTGAAATATTAAAGGTGATTGGTCGAGGAGCTTTTGGGGAGGTTGCTGTAG TAAAACTAAAAAATGCAGATAAAGTGTTTGCCATGAAAATATTGAATAAATGGGAAATGCTGAAAAGAGC TGAGACAGCATGTTTTCGTGAAGAAAGGGATGTATTAGTGAATGGAGACAATAAATGGATTACAACCTTG CACTATGCTTTCCAGGATGACAATAACTTATACCTGGTTATGGATTATTATGTTGGTGGGGATTTGCTTA CTCTACTCAGCAAATTTGAAGATAGATTGCCTGAAGATATGGCTAGATTTTACTTGGCTGAGATGGTGAT AGCAATTGACTCAGTTCATCAGCTACATTATGTACACAGAGACATTAAACCTGACAATATACTGATGGAT ATGAATGGACATATTCGGTTAGCAGATTTTGGTTCTTGTCTGAAGCTGATGGAAGATGGAACGGTTCAGT CCTCAGTGGCTGTAGGAACTCCAGATTATATCTCTCCTGAAATCCTTCAAGCCATGGAAGATGGAAAAGG GAGATATGGACCTGAATGTGACTGGTGGTCTTTGGGGGTCTGTATGTATGAAATGCTTTACGGAGAAACA CCATTTTATGCAGAATCGCTGGTGGAGACATACGGAAAAATCATGAACCACAAAGAGAGGTTTCAGTTTC CAGCCCAAGTGACTGATGTGTCTGAAAATGCTAAGGATCTTATTCGAAGGCTCATTTGTAGCAGAGAACA TCGACTTGGTCAAAATGGAATAGAAGACTTTAAGAAACACCCATTTTTCAGTGGAATTGATTGGGATAAT ATTCGGAACTGTGAAGCACCTTATATTCCAGAAGTTAGTAGCCCAACAGATACATCGAATTTTGATGTAG ATGATGATTGTTTAAAAAATTCTGAAACGATGCCCCCACCAACACATACTGCATTTTCTGGCCACCATCT GCCATTTGTTGGTTTTACATATACTAGTAGCTGTGTACTTTCTGATCGGAGCTGTTTAAGAGTTACGGCT GGTCCCACCTCACTGGATCTTGATGTTAATGTTCAGAGGACTCTAGACAACAACTTAGCAACTGAAGCTT ATGAAAGAAGAATTAAGCGCCTTGAGCAAGAAAAACTTGAACTCAGTAGAAAACTTCAAGAGTCAACACA GACTGTCCAAGCTCTGCAGTATTCAACTGTTGATGGTCCACTAACAGCAAGCAAAGATTTAGAAATAAAA AACTTAAAAGAAGAAATTGAAAAACTAAGAAAACAAGTAACAGAATCAAGTCATTTGGAACAGCAACTTG AAGAAGCTAATGCTGTGAGGCAAGAACTAGATGATGCTTTTAGACAAATCAAGGCTTATGAAAAACAAAT CAAAACGTTACAACAAGAAAGAGAAGATCTAAATAAGGAACTAGTCCAGGCTAGTGAGCGATTAAAAAAC CAATCCAAAGAGCTGAAAGACGCACACTGTCAGAGGAAACTGGCCATGCAGGAATTCATGGAGATCAATG AGCGGCTAACAGAATTGCACACCCAAAAACAGAAACTTGCTCGCCATGTCCGAGATAAGGAAGAAGAGGT GGACCTGGTGATGCAAAAAGTTGAAAGCTTAAGGCAAGAACTGCGCAGAACAGAAAGAGCCAAAAAAGAG CTGGAAGTTCATACAGAAGCTCTAGCTGCTGAAGCATCTAAAGACAGGAAGCTACGTGAACAGAGTGAGC ACTATTCTAAGCAACTGGAAAATGAATTGGAGGGACTGAAGCAAAAACAAATTAGTTACTCACCAGGAGT ATGCAGCATAGAACATCAGCAAGAGATAACCAAACTAAAGACTGATTTGGAAAAGAAAAGTATCTTTTAT GAAGAAGAATTATCTAAAAGAGAAGGAATACATGCAAATGAAATAAAAAATCTTAAGAAAGAACTGCATG ATTCAGAAGGTCAGCAACTTGCTCTCAACAAAGAAATTATGATTTTAAAAGACAAATTGGAAAAAACCAG AAGAGAAAGTCAAAGTGAAAGGGAGGAATTTGAAAGTGAGTTCAAACAACAATATGAACGAGAAAAAGTG TTGTTAACTGAAGAAAATAAAAAGCTGACGAGTGAACTTGATAAGCTTACTACTTTGTATGAGAACTTAA GTATACACAACCAGCAGTTAGAAGAAGAGGTTAAAGATCTAGCAGACAAGAAAGAATCAGTTGCACATTG GGAAGCCCAAATCACAGAAATAATTCAGTGGGTCAGCGATGAAAAGGATGCACGAGGGTATCTTCAGGCC TTAGCTTCTAAAATGACTGAAGAATTGGAGGCATTAAGAAATTCCAGCTTGGGTACACGAGCAACAGATA TGCCCTGGAAAATGCGTCGTTTTGCGAAACTGGATATGTCAGCTAGACTGGAGTTGCAGTCGGCTCTGGA TGCAGAAATAAGAGCCAAACAGGCCATCCAAGAAGAGTTGAATAAAGTTAAAGCATCTAATATCATAACA GAATGTAAACTAAAAGATTCAGAGAAGAAGAACTTGGAACTACTCTCAGAAATCGAACAGCTGATAAAGG ACACTGAAGAGCTTAGATCTGAAAAGGGTATAGAGCACCAAGACTCACAGCATTCTTTCTTGGCATTTTT GAATACGCCTACCGATGCTCTGGATCAATTTGAAACTGATCCCGTTGAGAACACATATGTATGGAACCCG AGCGTCAAGTTTCACATCCAGTCACGGTCCACATCTCCTTCCACATCTAGTGAAGCTGAGCCAGTTAAGA CTGTAGACTCCACTCCACTTTCAGTTCACACACCAACCTTAAGGAAAAAAGGATGTCCTGGTTCAACTGG CTTTCCACCTAAGCGCAAGACTCACCAGTTTTTTGTAAAATCTTTTACTACTCCTACCAAGTGTCATCAG TGTACCTCCTTGATGGTGGGTTTAATAAGACAGGGCTGTTCATGTGAAGTGTGTGGATTCTCATGCCATA TAACTTGTGTAAACAAAGCTCCAACCACTTGTCCAGTTCCTCCTGAACAGACAAAAGGTCCCCTGGGTAT AGATCCTCAGAAAGGAATAGGAACAGCATATGAAGGTCATGTCAGGATTCCTAAGCCAGCTGGAGTGAAG AAAGGGTGGCAGAGAGCACTGGCTATAGTGTGTGACTTCAAACTCTTTCTGTACGATATTGCTGAAGGAA AAGCATCTCAGCCCAGTGTTGTCATTAGTCAAGTGATTGACATGAGGGATGAAGAATTTTCTGTGAGTTC AGTCTTGGCTTCTGATGTTATCCATGCAAGTCGGAAAGATATACCCTGTATATTTAGGGTCACAGCTTCC CAGCTCTCAGCATCTAATAACAAATGTTCAATCCTGATGCTAGCAGACACTGAGAATGAGAAGAATAAGT GGGTGGGAGTGCTGAGTGAATTGCACAAGATTTTGAAGAAAAACAAATTCAGAGACCGCTCAGTCTATGT TCCCAAAGAGGCTTATGACAGCACTCTACCCCTCATTAAAACAACCCAGGCAGCCGCAATCATAGATCAT GAAAGAATTGCTTTGGGAAACGAAGAAGGGTTATTTGTTGTACATGTCACCAAAGATGAAATTATTAGAG TTGGTGACAATAAGAAGATTCATCAGATTGAACTCATTCCAAATGATCAGCTTGTTGCTGTGATCTCAGG ACGAAATCGTCATGTACGACTTTTTCCTATGTCAGCATTGGATGGGCGAGAGACCGATTTTTACAAGCTG TCAGAAACTAAAGGGTGTCAAACCRTAACTTCTGGAAAGGTGCGCCATGGAGCTCTCACATGCCTGTGTG TGGCTATGAAAAGGCAGGTCCTCTGTTATGAACTATTTCAGAGCAAGACCCGTCACAGAAAATTTAAAGA AATTCAAGTCCCATATAATGTCCAGTGGATGGCAATCTTCAGTGAACAACTCTGTGTGGGATTCCAGTCA GGATTTCTAAGATACCCCTTGAATGGAGAAGGAAATCCATACAGTATGCTCCATTCAAATGACCATACAC TATCATTTATTGCACATCAACCAATGGATGCTATCTGCGCAGTTGAGATCTCCAGTAAAGAATATCTGCT GTGTTTTAACAGCATTGGGATATACACTGACTGCCAGGGCCGAAGATCTAGACAACAGGAATTGATGTGG CCAGCAAATCCTTCCTCTTGTTGTTACAATGCACCATATCTCTCGGTGTACAGTGAAAATGCAGTTGATA TCTTTGATGTGAACTCCATGGAATGGATTCAGACTCTTCCTCTCAAAAAGGTTCGACCCTTAAACAATGA AGGATCATTAAATCTTTTAGGGTTGGAGACCATTAGATTAATATATTTCAAAAATAAGATGGCAGAAGGG GACGAACTGGTAGTACCTGAAACATCAGATAATAGTCGGAAACAAATGGTTAGAAACATTAACAATAAGC GGCGTTATTCCTTCAGAGTCCCAGAAGAGGAAAGGATGCAGCAGAGGAGGGAAATGCTACGAGATCCAGA AATGAGAAATAAATTAATTTCTAATCCAACTAATTTTAATCACATAGCACACATGGGTCCTGGAGATGGA ATACAGATCCTGAAAGATCTGCCCATGAACCCTCGGCCTCAGGAAAGTCGGACAGTATTCAGTGGCTCAG TCAGTATTCCATCTATCACCAAATCCCGCCCTGAGCCAGGCCGCTCCATGAGTGCTAGCAGTGGCTTGTC AGCAAGGTCATCCGCACAGAATGGCAGCGCATTAAAGAGGGAATTCTCTGGAGGAAGCTACAGTGCCAAG CGGCAGCCCATGCCCTCCCCGTCAGAGGGCTCTTTGTCCTCCGGAGGCATGGACCAAGGAAGTGATGCCC CAGCGAGGGACTTTGACGGAGAGGACTCTGACTCTCCGAGGCATTCCACAGCTTCCAACAGTTCCAACCT AAGCAGCCCCCCAAGCCCAGTTTCACCCCGAAAAACCAAGAGCCTCTCCCTGGAGAGCACTGACCGCGGG AGCTGGGACCCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214083 representing NM_003607
Red=Cloning site Green=Tags(s) MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQM RLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDNKWITTL HYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEDMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMD MNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGET PFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDN IRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTA GPTSLDLDVNVQRTLDNNLATEAYERRIKRLEQEKLELSRKLQESTQTVQALQYSTVDGPLTASKDLEIK NLKEEIEKLRKQVTESSHLEQQLEEANAVRQELDDAFRQIKAYEKQIKTLQQEREDLNKELVQASERLKN QSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKE LEVHTEALAAEASKDRKLREQSEHYSKQLENELEGLKQKQISYSPGVCSIEHQQEITKLKTDLEKKSIFY EEELSKREGIHANEIKNLKKELHDSEGQQLALNKEIMILKDKLEKTRRESQSEREEFESEFKQQYEREKV LLTEENKKLTSELDKLTTLYENLSIHNQQLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQA LASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNIIT ECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFETDPVENTYVWNP SVKFHIQSRSTSPSTSSEAEPVKTVDSTPLSVHTPTLRKKGCPGSTGFPPKRKTHQFFVKSFTTPTKCHQ CTSLMVGLIRQGCSCEVCGFSCHITCVNKAPTTCPVPPEQTKGPLGIDPQKGIGTAYEGHVRIPKPAGVK KGWQRALAIVCDFKLFLYDIAEGKASQPSVVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTAS QLSASNNKCSILMLADTENEKNKWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDH ERIALGNEEGLFVVHVTKDEIIRVGDNKKIHQIELIPNDQLVAVISGRNRHVRLFPMSALDGRETDFYKL SETKGCQTXTSGKVRHGALTCLCVAMKRQVLCYELFQSKTRHRKFKEIQVPYNVQWMAIFSEQLCVGFQS GFLRYPLNGEGNPYSMLHSNDHTLSFIAHQPMDAICAVEISSKEYLLCFNSIGIYTDCQGRRSRQQELMW PANPSSCCYNAPYLSVYSENAVDIFDVNSMEWIQTLPLKKVRPLNNEGSLNLLGLETIRLIYFKNKMAEG DELVVPETSDNSRKQMVRNINNKRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDG IQILKDLPMNPRPQESRTVFSGSVSIPSITKSRPEPGRSMSASSGLSARSSAQNGSALKREFSGGSYSAK RQPMPSPSEGSLSSGGMDQGSDAPARDFDGEDSDSPRHSTASNSSNLSSPPSPVSPRKTKSLSLESTDRG SWDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003607 |
| ORF Size | 1424 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 8019 bp |
| RefSeq ORF | 5160 bp |
| Locus ID | 8476 |
| UniProt ID | Q5VT25 |
| Domains | pkinase, S_TK_X, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 199.86 kDa |
| Gene Summary | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described. [provided by RefSeq, Sep 2018] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214083L3 | Lenti ORF clone of Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant B, Myc-DDK-tagged |
CNY 15,680.00 |
|
| RG214083 | CDC42BPA (tGFP-tagged) - Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant B |
CNY 15,200.00 |
|
| SC309894 | CDC42BPA (untagged)-Human CDC42 binding protein kinase alpha (DMPK-like) (CDC42BPA), transcript variant B |
CNY 33,060.00 |
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