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DLG5 (NM_004747) Human Tagged ORF Clone
CAT＃: RC213793
- TrueORF®
DLG5 (Myc-DDK-tagged)-Human discs, large homolog 5 (Drosophila) (DLG5)
ORF Plasmid: tGFP
"NM_004747" in other vectors (4)
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CNY 19,344.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LP-DLG; P-DLG5; PDLG |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213793 representing NM_004747
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCCAGCGCCGGGAGCTGCTCGCCCAGTGTCAGCAGAGCCTGGCCCAGGCCATGACGGAGGTGG AAGCCGTGCTCGGGCTGCTCGAGGCCGCGGGAGCGCTCAGTCCCGGCGAGCGGCGGCAGCTGGACGAGGA GGCGGGAGGCGCCAAGGCGGAGCTGCTGCTCAAGCTGCTCTTGGCCAAGGAGCGGGACCACTTCCAGGAC CTGCGGGCGGCGCTGGAGAAGACGCAGCCTCACCTGCTGCCCATTCTCTACCTGAACGGCGTCGTCGGGC CGCCGCAGCCCGCCGAAGGCGCGGGTTCTACCTACAGCGTCCTGTCCACCATGCCCTCAGACTCAGAAAG CAGCAGCTCCCTCAGCAGTGTGGGCACTACCGGGAAGGCGCCGTCCCCACCACCCCTCCTCACTGACCGG CAAGTGAATGAGAAGGTGGAGAACCTCTCCATTCAGCTGCGGCTGATGACCCGGGAGAGAAACGAGCTCC GCAAGCGCCTGGCCTTTGCTACGCATGGCACGGCCTTTGACAAGAGGCCCTACCACAGGCTGAATCCTGA CTATGAGAGGCTGAAGATCCAGTGCGTGCGAGCCATGTCGGACCTGCAGAGCCTGCAGAACCAGCACACC AACGCCTTGAAGAGGTGTGAGGAGGTGGCCAAGGAGACTGACTTCTACCACACACTCCACAGCCGGCTCC TGAGTGACCAGACTCGGCTGAAGGATGACGTGGACATGCTGAGGCGGGAGAATGGGCAGCTGCTGCGGGA GCGAAACCTGCTGCAGCAGTCATGGGAGGACATGAAGCGGCTCCACGAGGAGGACCAGAAGGAGATCGGT GACCTCCGTGCCCAGCAGCAGCAGGTGTTGAAGCACAACGGGTCATCCGAGATTCTCAACAAACTGTATG ACACGGCCATGGACAAGTTGGAGGTGGTCAAGAAGGACTATGACGCCCTTCGGAAGAGGTACAGTGAGAA AGTCGCCATCCACAATGCAGACCTGAGCCGCCTGGAGCAGCTGGGGGAGGAGAACCAGCGGTTGCTGAAG CAGACAGAGATGCTGACCCAGCAGAGGGACACGGCCATCCAGCTGCAGCACCAGTGCGCCCTCTCCCTGA GGAGGTTTGAGGCGATCCACCATGAGCTGAACAAGGCCACGGCGCAGAACAAGGACCTGCAGTGGGAGAT GGAGCTGCTGCAGTCAGAGCTGACCGAGCTGAGAACCACGCAGGTGAAGACAGCAAAGGAGTCGGAGAAA TACAGGGAGGAGCGGGACGCTGTGTACAGCGAGTACAAGCTCATCATGAGTGAGCGTGACCAGGTCATCT CTGAGCTGGACAAGCTGCAGACCGAAGTGGAGCTGGCCGAGTCCAAGCTCAAGAGCAGCACATCTGAGAA GAAGGCGGCCAATGAGGAGATGGAGGCGCTGCGGCAGATCAAAGACACGGTGACAATGGATGCTGGGAGA GCCAACAAGGAGGTTGAAATCCTTCGAAAGCAGTGCAAGGCTCTGTGCCAGGAGCTGAAGGAAGCCCTCC AGGAGGCGGATGTGGCCAAGTGCCGGCGGGACTGGGCCTTCCAGGAGCGAGACAAGATTGTAGCAGAGCG TGACAGCATCCGGACACTGTGTGACAACCTGAGGCGGGAGCGGGACCGTGCGGTGAGCGAGCTGGCTGAG GCCCTGCGCAGCCTGGATGACACCCGCAAGCAGAAGAATGATGTCAGCCGCGAGCTGAAGGAGCTCAAGG AACAGATGGAATCCCAGTTGGAAAAGGAGGCCCGGTTCCGACAGCTGATGGCCCACAGCTCCCACGACTC GGCCATTGACACGGATTCCATGGAGTGGGAAACGGAAGTTGTAGAGTTCGAGAGGGAGACGGAGGATATT GACTTGAAGGCACTGGGGTTTGATATGGCAGAAGGTGTGAATGAGCCTTGTTTCCCGGGGGACTGTGGCA TATTTGTCACTAAAGTGGACAAAGGAAGCATTGCTGATGGCCGCTTAAGGGTCAATGACTGGCTGCTGAG AATCAACGATGTGGACCTCATCAACAAGGACAAGAAGCAGGCCATCAAGGCGCTCCTCAATGGGGAGGGG GCCATCAACATGGTCGTGCGGCGGAGGAAGTCCCTGGGTGGGAAGGTGGTCACGCCGCTGCACATCAACC TCAGTGGACAGAAAGACAGTGGCATCAGTCTGGAGAATGGAGTGTATGCTGCCGCTGTGCTGCCTGGAAG CCCTGCCGCTAAAGAAGGGTCCCTTGCTGTGGGAGACAGGATCGTTGCGATCAATGGCATTGCACTGGAC AACAAGTCTCTGAATGAATGTGAATCTCTGCTGCGGAGCTGCCAGGACTCCCTGACCCTGTCCCTCCTGA AGGTATTCCCTCAGAGCTCCTCGTGGAGTGGCCAGAACATTTTTGAAAATATCAAAGACTCTGATAAGAT GCTGAGTTTTCGAGCCCATGGCCCGGAGGTCCAGGCTCATAACAAACGGAACTTGATACAGCACAATAAC TCCACGCAGACAGACATCTTCTACACGGACAGGCTGGAAGACAGGAAGGAGCCAGGCCCCCCAGGAGGCA GCAGCTCCTTTCTGCATAAGCCATTCCCTGGGGGACCCTTGCAGGTCTGCCCCCAGGCCTGTCCCAGTGC CTCTGAGCGTAGCCTGAGCTCCTTCCGCTCAGATGCCTCTGGGGACCGTGGCTTTGGGCTGGTGGACGTG CGTGGCCGGCGGCCACTGCTGCCCTTTGAGACCGAGGTGGGCCCCTGTGGGGTTGGGGAGGCCTCCCTGG ACAAGGCAGACTCTGAAGGCTCCAACAGCGGCGGGACCTGGCCCAAGGCCATGCTCAGCTCCACGGCAGT GCCTGAGAAGCTCTCTGTTTATAAAAAGCCAAAGCAAAGAAAGTCCATCTTTGACCCTAACACTTTCAAA CGCCCCCAGACACCCCCCAAAATAGACTACCTGCTTCCAGGTCCTGGGCCTGCTCACTCTCCCCAGCCCT CCAAGAGGGCGGGGCCTCTGACACCCCCAAAACCTCCCAGAAGGAGCGACTCCATTAAGTTCCAGCACAG GCTGGAGACTAGCTCCGAGTCAGAAGCCACTCTGGTGGGCAGCTCCCCATCCACTAGTCCCCCGAGCGCC CTGCCCCCTGACGTGGACCCCGGGGAGCCCATGCACGCATCACCCCCTCGCAAGGCCAGGGTCCGCATTG CTTCCAGCTACTACCCTGAAGGAGATGGGGACTCCTCCCACCTGCCGGCCAAGAAATCCTGTGATGAGGA CCTCACCTCCCAGAAGGTGGATGAGCTGGGGCAGAAGCGTCGCCGGCCAAAATCTGCTCCCAGTTTTCGG CCGAAGCTTGCTCCAGTAGTGATTCCTGCTCAGTTCCTGGAGGAACAGAAGTGTGTCCCGGCCAGTGGAG AACTCTCCCCGGAGCTCCAGGAGTGGGCACCTTACTCGCCTGGGCATTCCAGCCGGCACAGCAACCCCCC GCTATACCCTAGCAGGCCGTCTGTGGGCACTGTTCCCCGGAGTTTGACCCCCAGCACCACTGTGAGCTCC ATCCTGCGGAACCCCATCTACACTGTGCGCAGTCACAGGGTCGGCCCCTGCAGCTCTCCACCTGCGGCCC GAGATGCTGGCCCCCAGGGTTTGCATCCCAGTGTCCAGCACCAGGGACGCCTGAGCCTGGACCTGAGCCA CAGGACCTGCAGCGACTACTCCGAGATGAGAGCCACCCATGGGTCCAACTCACTGCCCTCCAGCGCCCGC CTGGGTTCTTCGAGTAACTTGCAGTTCAAGGCGGAACGCATTAAAATCCCATCAACACCAAGATATCCGC GGAGTGTCGTGGGCTCTGAGAGAGGTTCAGTGTCACATTCTGAATGCAGCACTCCTCCACAGTCACCCCT GAACATCGACACCCTGTCCTCTTGTAGCCAGTCCCAGACCTCAGCCTCCACATTGCCCAGAATCGCTGTC AACCCCGCGTCCCTCGGGGAGCGGAGAAAGGACAGGCCTTATGTGGAGGAGCCACGCCACGTGAAGGTGC AGAAGGGCTCAGAGCCGCTGGGCATCTCCATCGTGAGTGGAGAGAAGGGCGGCATCTACGTCTCCAAGGT GACCGTGGGGAGCATCGCTCACCAGGCTGGCCTCGAGTATGGGGATCAGTTACTGGAGTTCAACGGCATA AACCTGCGGAGCGCCACGGAGCAGCAGGCGCGGCTCATCATCGGGCAGCAGTGTGATACCATCACCATCC TGGCCCAGTACAACCCCCACGTGCACCAGCTCAGCAGCCACTCCCGGTCCAGCTCACACCTGGACCCTGC CGGTACCCACTCCACTCTCCAGGGCAGTGGCACCACCACCCCGGAGCATCCATCTGTCATCGACCCACTG ATGGAGCAGGACGAGGGGCCTAGCACCCCCCCAGCCAAGCAGAGCAGCTCCAGGATTGCGGGAGATGCCA ACAAGAAGACCCTGGAGCCACGCGTTGTCTTCATCAAAAAGTCCCAGCTGGAGCTTGGGGTGCACTTGTG TGGTGGGAACCTGCATGGGGTGTTTGTGGCCGAGGTGGAGGATGACAGTCCTGCCAAGGGTCCTGACGGC CTCGTGCCAGGGGACCTCATCCTGGAGTATGGCAGCCTGGACGTGCGGAACAAGACAGTGGAGGAAGTCT ATGTGGAGATGCTGAAGCCCAGGGATGGCGTCCGCCTGAAGGTGCAGTACCGCCCTGAGGAGTTCACGAA GGCCAAGGGCCTGCCTGGTGACAGCTTCTACATCAGGGCCCTGTACGACCGGCTGGCAGATGTGGAGCAA GAGTTGAGCTTTAAGAAGGATGACATCCTCTACGTGGATGACACCTTACCCCAGGGCACGTTCGGGTCCT GGATGGCTTGGCAGCTGGACGAGAATGCCCAGAAGATCCAGCGCGGGCAGATTCCCAGCAAATATGTGAT GGACCAAGAATTCTCCAGGAGGCTCAGCATGTCTGAAGTCAAAGATGACAATAGCGCCACAAAGACGCTG TCAGCGGCTGCACGCCGGTCCTTTTTTCGGAGGAAACACAAGCACAAACGCAGCGGGTCCAAGGACGGGA AAGACCTGCTCGCCTTGGATGCCTTTTCCAGTGACTCCATTCCACTCTTTGAAGATTCGGTGAGCCTGGC CTATCAGCGGGTCCAGAAGGTGGACTGCACCGCTCTGAGGCCTGTCCTGATTCTGGGGCCTTTGCTGGAC GTGGTGAAGGAGATGCTGGTGAATGAGGCTCCTGGCAAGTTCTGCAGATGTCCCCTTGAGGTGATGAAGG CCTCCCAGCAGGCCATTGAGCGGGGTGTCAAAGATTGCCTGTTTGTCGACTATAAGCGGAGAAGCGGCCA TTTCGATGTGACCACTGTGGCGTCAATAAAGGAGATCACAGAAAAGAACCGACACTGCCTCCTGGACATT GCTCCGCACGCTATTGAGCGGCTCCACCACATGCACATCTACCCTATTGTCATCTTCATCCACTACAAGA GCGCCAAGCACATCAAGGAGCAGAGAGACCCCATCTACCTGAGGGACAAGGTGACTCAGAGGCATTCCAA AGAGCAGTTTGAGGCGGCGCAGAAGCTTGAGCAGGAGTACAGCAGGTACTTCACAGGGGTCATCCAGGGA GGAGCCCTGTCAAGCATTTGCACTCAGATCTTGGCAATGGTCAATCAAGAACAAAATAAAGTCCTGTGGA TTCCAGCCTGCCCGCTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC213793 representing NM_004747
Red=Cloning site Green=Tags(s) MEPQRRELLAQCQQSLAQAMTEVEAVLGLLEAAGALSPGERRQLDEEAGGAKAELLLKLLLAKERDHFQD LRAALEKTQPHLLPILYLNGVVGPPQPAEGAGSTYSVLSTMPSDSESSSSLSSVGTTGKAPSPPPLLTDR QVNEKVENLSIQLRLMTRERNELRKRLAFATHGTAFDKRPYHRLNPDYERLKIQCVRAMSDLQSLQNQHT NALKRCEEVAKETDFYHTLHSRLLSDQTRLKDDVDMLRRENGQLLRERNLLQQSWEDMKRLHEEDQKEIG DLRAQQQQVLKHNGSSEILNKLYDTAMDKLEVVKKDYDALRKRYSEKVAIHNADLSRLEQLGEENQRLLK QTEMLTQQRDTAIQLQHQCALSLRRFEAIHHELNKATAQNKDLQWEMELLQSELTELRTTQVKTAKESEK YREERDAVYSEYKLIMSERDQVISELDKLQTEVELAESKLKSSTSEKKAANEEMEALRQIKDTVTMDAGR ANKEVEILRKQCKALCQELKEALQEADVAKCRRDWAFQERDKIVAERDSIRTLCDNLRRERDRAVSELAE ALRSLDDTRKQKNDVSRELKELKEQMESQLEKEARFRQLMAHSSHDSAIDTDSMEWETEVVEFERETEDI DLKALGFDMAEGVNEPCFPGDCGIFVTKVDKGSIADGRLRVNDWLLRINDVDLINKDKKQAIKALLNGEG AINMVVRRRKSLGGKVVTPLHINLSGQKDSGISLENGVYAAAVLPGSPAAKEGSLAVGDRIVAINGIALD NKSLNECESLLRSCQDSLTLSLLKVFPQSSSWSGQNIFENIKDSDKMLSFRAHGPEVQAHNKRNLIQHNN STQTDIFYTDRLEDRKEPGPPGGSSSFLHKPFPGGPLQVCPQACPSASERSLSSFRSDASGDRGFGLVDV RGRRPLLPFETEVGPCGVGEASLDKADSEGSNSGGTWPKAMLSSTAVPEKLSVYKKPKQRKSIFDPNTFK RPQTPPKIDYLLPGPGPAHSPQPSKRAGPLTPPKPPRRSDSIKFQHRLETSSESEATLVGSSPSTSPPSA LPPDVDPGEPMHASPPRKARVRIASSYYPEGDGDSSHLPAKKSCDEDLTSQKVDELGQKRRRPKSAPSFR PKLAPVVIPAQFLEEQKCVPASGELSPELQEWAPYSPGHSSRHSNPPLYPSRPSVGTVPRSLTPSTTVSS ILRNPIYTVRSHRVGPCSSPPAARDAGPQGLHPSVQHQGRLSLDLSHRTCSDYSEMRATHGSNSLPSSAR LGSSSNLQFKAERIKIPSTPRYPRSVVGSERGSVSHSECSTPPQSPLNIDTLSSCSQSQTSASTLPRIAV NPASLGERRKDRPYVEEPRHVKVQKGSEPLGISIVSGEKGGIYVSKVTVGSIAHQAGLEYGDQLLEFNGI NLRSATEQQARLIIGQQCDTITILAQYNPHVHQLSSHSRSSSHLDPAGTHSTLQGSGTTTPEHPSVIDPL MEQDEGPSTPPAKQSSSRIAGDANKKTLEPRVVFIKKSQLELGVHLCGGNLHGVFVAEVEDDSPAKGPDG LVPGDLILEYGSLDVRNKTVEEVYVEMLKPRDGVRLKVQYRPEEFTKAKGLPGDSFYIRALYDRLADVEQ ELSFKKDDILYVDDTLPQGTFGSWMAWQLDENAQKIQRGQIPSKYVMDQEFSRRLSMSEVKDDNSATKTL SAAARRSFFRRKHKHKRSGSKDGKDLLALDAFSSDSIPLFEDSVSLAYQRVQKVDCTALRPVLILGPLLD VVKEMLVNEAPGKFCRCPLEVMKASQQAIERGVKDCLFVDYKRRSGHFDVTTVASIKEITEKNRHCLLDI APHAIERLHHMHIYPIVIFIHYKSAKHIKEQRDPIYLRDKVTQRHSKEQFEAAQKLEQEYSRYFTGVIQG GALSSICTQILAMVNQEQNKVLWIPACPL SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004747 |
| ORF Size | 5757 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004747.3, NP_004738.3 |
| RefSeq Size | 7493 bp |
| RefSeq ORF | 5760 bp |
| Locus ID | 9231 |
| UniProt ID | Q8TDM6 |
| Domains | SH3, PDZ, Guanylate_kin, GuKc |
| Protein Families | Druggable Genome |
| MW | 213.7 kDa |
| Gene Summary | This gene encodes a member of the family of discs large (DLG) homologs, a subset of the membrane-associated guanylate kinase (MAGUK) superfamily. The MAGUK proteins are composed of a catalytically inactive guanylate kinase domain, in addition to PDZ and SH3 domains, and are thought to function as scaffolding molecules at sites of cell-cell contact. The protein encoded by this gene localizes to the plasma membrane and cytoplasm, and interacts with components of adherens junctions and the cytoskeleton. It is proposed to function in the transmission of extracellular signals to the cytoskeleton and in the maintenance of epithelial cell structure. Alternative splice variants have been described but their biological nature has not been determined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213793L1 | Lenti ORF clone of Human discs, large homolog 5 (Drosophila) (DLG5), Myc-DDK-tagged |
CNY 16,060.00 |
|
| RG213793 | DLG5 (tGFP-tagged) - Human discs, large homolog 5 (Drosophila) (DLG5) |
CNY 15,580.00 |
|
| SC303527 | DLG5 (untagged)-Human discs, large homolog 5 (Drosophila) (DLG5) |
CNY 26,790.00 |
|
| SC318695 | DLG5 (untagged)-Human discs, large homolog 5 (Drosophila) (DLG5) |
CNY 26,790.00 |
