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GCN2 (EIF2AK4) (NM_001013703) Human Tagged ORF Clone
CAT＃: RC212459
- TrueORF®
EIF2AK4 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4)
ORF Plasmid: tGFP
"NM_001013703" in other vectors (4)
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CNY 16,920.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GCN2; PVOD2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212459 representing NM_001013703
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGGGCCGTGGGGCCCCCGGGCGCGGCCGGGACGAGCCTCCGGAGAGCTACCCGCAACGACAGG ACCACGAGCTACAGGCCCTGGAGGCCATCTACGGCGCGGACTTCCAAGACCTGCGGCCGGACGCTTGCGG ACCGGTCAAAGAGCCCCCTGAAATCAATTTAGTTTTGTACCCTCAAGGCCTAACTGGTGAAGAAGTATAT GTAAAAGTGGATTTGAGGGTTAAATGCCCACCTACCTATCCAGATGTAGTTCCTGAAATAGAGTTAAAAA ATGCCAAAGGTCTATCAAATGAAAGTGTCAATTTGTTAAAATCTCGCCTAGAAGAACTGGCCAAGAAACA CTGTGGGGAGGTGATGATCTTTGAACTGGCTTACCACGTGCAGTCATTTCTCAGCGAGCATAACAAGCCC CCTCCCAAGTCTTTTCATGAAGAAATGCTGGAAAGGCGGGCTCAGGAGGAGCAGCAGAGGCTGTTGGAGG CCAAGCGGAAAGAAGAGCAGGAGCAACGTGAAATCCTGCATGAGATTCAGAGAAGGAAAGAAGAGATAAA AGAAGAGAAAAAAAGGAAAGAAATGGCTAAGCAGGAACGTTTGGAAATTGCTAGTTTGTCAAACCAAGAT CATACCTCTAAGAAGGACCCAGGAGGACACAGAACGGCTGCCATTCTACATGGAGGCTCTCCTGACTTTG TAGGAAATGGTAAACATCGGGCAAACTCCTCAGGAAGGTCTAGGCGAGAACGTCAGTATTCTGTATGTAA TAGTGAAGATTCTCCTGGCTCTTGTGAAATTCTGTATTTCAATATGGGGAGTCCTGATCAGCTCATGGTG CACAAAGGGAAATGTATTGGCAGTGATGAACAACTTGGAAAATTAGTCTACAATGCTTTGGAAACAGCCA CTGGTGGCTTTGTCTTGTTGTATGAGTGGGTCCTTCAGTGGCAGAAAAAAATGGGTCCATTCCTTACCAG TCAAGAAAAAGAGAAGATTGATAAGTGCAAAAAGCAGATTCAAGGAACAGAAACAGAATTCAACTCACTG GTAAAATTGAGCCATCCAAATGTAGTACGCTACCTTGCAATGAATCTCAAAGAGCAAGACGACTCCATCG TGGTGGACATTTTAGTGGAGCACATTAGTGGGGTCTCTCTTGCTGCACACCTGAGCCACTCAGGCCCCAT CCCTGTGCATCAGCTTCGCAGGTACACAGCTCAGCTCCTGTCAGGCCTTGATTATCTGCACAGCAATTCT GTGGTGCATAAGGTCCTGAGTGCATCTAATGTCTTGGTGGATGCAGAAGGCACCGTCAAGATTACGGACT ATAGCATTTCTAAGCGCCTCGCAGACATTTGCAAGGAGGATGTGTTTGAGCAAACCCGAGTTCGTTTTAG TGACAATGCTCTGCCTTATAAAACGGGGAAGAAAGGAGATGTTTGGCGTCTTGGCCTTCTGCTGCTGTCC CTCAGCCAAGGACAGGAATGTGGAGAGTACCCTGTGACCATCCCTAGTGACTTACCAGCTGACTTTCAAG ATTTTCTAAAGAAATGTGTGTGCTTGGATGACAAGGAAAGATGGAGTCCCCAGCAGTTGTTGAAACACAG CTTTATAAATCCCCAGCCAAAAATGCCTCTAGTGGAACAAAGTCCTGAAGATTCTGAAGGACAAGATTAT GTTGAGACTGTTATTCCTAGCAACCGGCTACCCAGTGCTGCCTTCTTTAGTGAGACACAGAGACAGTTTT CCCGATACTTCATTGAGTTTGAAGAATTACAACTTCTTGGTAAAGGAGCTTTTGGAGCTGTCATCAAGGT GCAGAACAAGTTGGACGGCTGCTGCTACGCAGTGAAGCGCATCCCCATCAACCCGGCCAGCCGGCAGTTC CGCAGGATCAAGGGCGAAGTGACACTGCTGTCACGGCTGCACCATGAGAACATTGTGCGCTACTACAACG CCTGGATCGAGCGGCACGAGCGGCCGGCGGGACCGGGGACGCCGCCCCCGGACTCCGGGCCCCTGGCCAA GGATGACCGAGCTGCACGCGGGCAGCCGGCGAGCGACACAGACGGCCTGGACAGCGTAGAGGCCGCCGCG CCGCCACCCATCCTCAGCAGCTCGGTGGAGTGGAGCACTTCGGGCGAGCGCTCGGCCAGTGCCCGTTTCC CCGCCACCGGCCCGGGCTCCAGCGATGACGAGGACGACGACGAGGACGAGCACGGTGGCGTCTTCTCCCA GTCCTTCCTGCCTGCTTCAGATTCTGAAAGTGATATTATCTTTGACAATGAAGATGAGAACAGTAAAAGT CAGAATCAGGATGAAGATTGCAATGAAAAGAATGGCTGCCATGAAAGTGAGCCATCAGTGACGACTGAGG CTGTGCACTACCTATACATCCAGATGGAGTACTGTGAGAAGAGCACTTTACGAGACACCATTGACCAGGG ACTGTATCGAGACACCGTCAGACTCTGGAGGCTTTTTCGAGAGATTCTGGATGGATTAGCTTATATCCAT GAGAAAGGAATGATTCACCGGGATTTGAAGCCTGTCAACATTTTTTTGGATTCTGATGACCATGTGAAAA TAGGTGATTTTGGTTTGGCGACAGACCATCTAGCCTTTTCTGCTGACAGCAAACAAGACGATCAGACAGG AGACTTGATTAAGTCAGACCCTTCAGGTCACTTAACTGGGATGGTTGGCACTGCTCTCTATGTAAGCCCA GAGGTCCAAGGAAGCACCAAATCTGCATACAACCAGAAAGTGGATCTCTTCAGCCTGGGAATTATCTTCT TTGAGATGTCCTATCACCCCATGGTCACGGCTTCAGAAAGGATCTTTGTTCTCAACCAACTCAGAGATCC CACTTCGCCTAAGTTTCCAGAAGACTTTGACGATGGAGAGCATGCAAAGCAGAAATCAGTCATCTCCTGG CTGTTGAACCACGATCCAGCAAAACGGCCCACAGCCACAGAACTGCTCAAGAGTGAGCTGCTGCCCCCAC CCCAGATGGAGGAGTCAGAGCTGCATGAAGTGCTGCACCACACGCTGACCAACGTGGATGGGAAGGCCTA CCGCACCATGATGGCCCAGATCTTCTCGCAGCGCATCTCCCCTGCCATCGATTACACCTATGACAGCGAC ATACTGAAGGGCAACTTCTCAATCCGTACAGCCAAGATGCAGCAGCATGTGTGTGAAACCATCATCCGCA TCTTTAAAAGACATGGAGCTGTTCAGTTGTGTACTCCACTACTGCTTCCCCGAAACAGACAAATATATGA GCACAACGAAGCTGCCCTATTCATGGACCACAGCGGGATGCTGGTGATGCTTCCTTTTGACCTGCGGATC CCTTTTGCAAGATATGTGGCAAGAAATAATATATTGAATTTAAAACGATACTGCATAGAACGTGTGTTCA GGCCGCGCAAGTTAGATCGATTTCATCCCAAAGAACTTCTGGAGTGTGCATTTGATATTGTCACTTCTAC CACCAACAGCTTTCTGCCCACTGCTGAAATTATCTACACTATCTATGAAATCATCCAAGAGTTTCCAGCA CTTCAGGAAAGAAATTACAGTATTTATTTGAACCATACCATGTTATTGAAAGCAATACTCTTACACTGTG GGATCCCAGAAGATAAACTCAGTCAAGTCTACATTATTCTGTATGATGCTGTGACAGAGAAGCTGACGAG GAGAGAAGTGGAAGCTAAATTTTGTAATCTGTCTTTGTCTTCTAATAGTCTGTGTCGACTCTACAAGTTT ATTGAACAGAAGGGAGATTTGCAAGATCTTATGCCAACAATAAATTCATTAATAAAACAGAAAACAGGTA TTGCACAGTTGGTGAAGTATGGCTTAAAAGACCTAGAGGAGGTTGTTGGACTGTTGAAGAAACTCGGCAT CAAGTTACAGGTCTTGATCAATTTGGGCTTGGTTTACAAGGTGCAGCAGCACAATGGAATCATCTTCCAG TTTGTGGCTTTCATCAAACGAAGGCAAAGGGCTGTACCTGAAATCCTCGCAGCTGGAGGCAGATATGACC TGCTGATTCCCCAGTTTAGAGGGCCACAAGCTCTGGGGCCAGTTCCCACTGCCATTGGGGTCAGCATAGC TATAGACAAGATATCTGCTGCTGTCCTCAACATGGAGGAATCTGTTACAATAAGCTCTTGTGACCTCCTG GTTGTAAGTGTTGGCCAGATGTCTATGTCCAGGGCCATCAACCTAACCCAGAAACTCTGGACAGCAGGCA TCACAGCAGAAATCATGTACGACTGGTCACAGTCCCAAGAGGAATTACAAGAGTACTGCAGACATCATGA AATCACCTATGTGGCCCTTGTCTCGGATAAAGAAGGAAGCCATGTCAAGGTTAAGTCTTTCGAGAAGGAA AGGCAGACAGAGAAGCGTGTGCTGGAGACTGAACTTGTGGACCATGTACTGCAGAAACTGAGGACTAAAG TCACTGATGAAAGGAATGGCAGAGAAGCTTCCGATAATCTTGCAGTGCAAAATCTGAAGGGGTCATTTTC TAATGCTTCAGGTTTGTTTGAAATCCATGGAGCAACAGTGGTTCCCATTGTGAGTGTGCTAGCCCCGGAG AAGCTGTCAGCCAGCACTAGGAGGCGCTATGAAACTCAGGTACAAACTCGACTTCAGACCTCCCTTGCCA ACTTACATCAGAAAAGCAGTGAAATTGAAATTCTGGCTGTGGATCTACCCAAAGAAACAATATTACAGTT TTTATCATTAGAGTGGGATGCTGATGAACAGGCATTTAACACAACTGTGAAGCAGCTGCTGTCACGCCTG CCAAAGCAAAGATACCTCAAATTAGTCTGTGATGAAATTTATAACATCAAAGTAGAAAAAAAGGTGTCTG TGCTATTTCTGTACAGCTATAGAGATGACTACTACAGAATCTTATTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212459 representing NM_001013703
Red=Cloning site Green=Tags(s) MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVY VKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKP PPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQD HTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMV HKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSL VKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNS VVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLS LSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDY VETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAA PPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKS QNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIH EKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSP EVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISW LLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSD ILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRI PFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPA LQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKF IEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQ FVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLL VVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKE RQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPE KLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRL PKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001013703 |
| ORF Size | 4947 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001013703.2, NP_001013725.2 |
| RefSeq Size | 5532 bp |
| RefSeq ORF | 4950 bp |
| Locus ID | 440275 |
| UniProt ID | Q9P2K8 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 187.4 kDa |
| Gene Summary | This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
GCN2 adapts protein synthesis to scavenging-dependent growth
,null,
Cell systems
,PubMed ID 34706266
[EIF2AK4]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212459L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), Myc-DDK-tagged |
CNY 19,320.00 |
|
| RC212459L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), Myc-DDK-tagged |
CNY 18,340.00 |
|
| RG212459 | EIF2AK4 (tGFP-tagged) - Human eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4) |
CNY 18,520.00 |
|
| SC301871 | EIF2AK4 (untagged)-Human eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4) |
CNY 11,288.00 |
