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RBM25 (NM_021239) Human Tagged ORF Clone
CAT＃: RC212256
- TrueORF®
RBM25 (Myc-DDK-tagged)-Human RNA binding motif protein 25 (RBM25)
ORF Plasmid: tGFP
"NM_021239" in other vectors (4)
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CNY 9,264.00
| Cited in 2 publications. |
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CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | fSAP94; NET52; RED120; RNPC7; S164; Snu71 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212256 representing NM_021239
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTTCCACCTCATTTGAATCGCCCTCCCATGGGAATCCCAGCACTCCCACCAGGGATCCCACCCC CGCAGTTTCCAGGATTTCCTCCACCTGTACCTCCAGGGACCCCAATGATTCCTGTACCAATGAGCATTAT GGCTCCTGCTCCAACTGTCTTAGTACCCACTGTGTCTATGGTTGGAAAGCATTTGGGCGCAAGAAAGGAT CATCCAGGCTTAAAGGCTAAAGAAAATGATGAAAATTGTGGTCCTACTACCACTGTTTTTGTTGGCAACA TTTCCGAGAAAGCTTCAGACATGCTTATAAGACAACTCTTAGCTAAATGTGGTTTGGTTTTGAGCTGGAA GAGAGTACAAGGTGCTTCCGGAAAGCTTCAAGCCTTCGGATTCTGTGAGTACAAGGAGCCAGAATCTACC CTCCGTGCACTCAGATTATTACATGACCTGCAAATTGGAGAGAAAAAGCTACTCGTTAAAGTTGATGCAA AGACAAAGGCACAGCTGGATGAATGGAAAGCAAAGAAGAAAGCTTCTAATGGGAATGCAAGGCCAGAAAC TGTCACTAATGACGATGAAGAAGCCTTGGATGAAGAAACAAAGAGGAGAGATCAGATGATTAAAGGGGCT ATTGAAGTTTTAATTCGTGAATACTCCAGTGAGCTAAATGCCCCCTCACAGGAATCTGATTCTCACCCCA GGAAGAAGAAGAAGGAAAAGAAGGAGGACATTTTCCGCAGATTTCCAGTGGCCCCACTGATCCCTTATCC ACTCATCACTAAGGAGGATATAAATGCTATAGAAATGGAAGAAGACAAAAGAGACCTGATATCTCGAGAG ATCAGCAAATTCAGAGACACACATAAGAAACTGGAAGAAGAGAAAGGCAAAAAGGAAAAAGAAAGACAGG AAATTGAGAAAGAGCGGAGAGAAAGAGAGAGGGAGCGTGAAAGGGAACGAGAAAGGCGAGAACGGGAACG AGAAAGGGAAAGAGAACGTGAACGAGAAAAGGAGAAAGAACGGGAGCGGGAACGAGAACGGGATAGGGAC CGTGACCGGACAAAAGAGAGAGACCGAGATCGGGATCGAGAGAGAGATCGTGACCGGGATAGAGAAAGGA GCTCAGATCGTAATAAGGATCGCAGTCGATCAAGAGAAAAAAGCAGAGATCGTGAAAGGGAACGAGAGCG GGAAAGAGAGAGAGAGAGAGAACGAGAGCGAGAACGAGAACGGGAGCGAGAGAGAGAGCGAGAGAGGGAA CGGGAGCGAGAAAGAGAAAAAGACAAAAAACGGGACCGAGAAGAAGATGAAGAAGATGCATACGAACGAA GAAAACTTGAAAGAAAACTCCGAGAGAAAGAAGCTGCTTATCAAGAGCGCCTTAAGAATTGGGAAATCAG AGAACGAAAGAAAACCCGGGAATATGAGAAAGAAGCTGAAAGAGAAGAAGAAAGAAGAAGAGAAATGGCC AAAGAAGCTAAACGACTAAAAGAATTCTTAGAAGACTATGATGATGATAGAGATGACCCCAAATATTACA GAGGAAGTGCTCTTCAGAAAAGGTTGCGTGATAGAGAAAAGGAAATGGAAGCAGATGAACGAGATAGGAA GAGAGAGAAGGAGGAGCTTGAGGAAATCAGGCAGCGCCTTCTGGCAGAAGGGCATCCAGATCCAGATGCA GAGCTCCAGAGGATGGAACAAGAGGCTGAGAGGCGCAGGCAGCCACAAATAAAGCAAGAGCCAGAATCAG AAGAGGAGGAAGAAGAAAAGCAAGAAAAAGAAGAAAAACGAGAAGAACCCATGGAAGAGGAAGAGGAACC AGAGCAAAAGCCTTGTCTGAAACCTACTCTGAGGCCCATCAGCTCTGCTCCATCTGTTTCCTCTGCCAGT GGCAATGCAACACCTAACACTCCTGGGGATGAGTCTCCCTGTGGTATTATTATTCCTCATGAAAACTCAC CAGATCAACAGCAACCTGAGGAGCATAGGCCAAAAATAGGACTAAGTCTTAAACTGGGTGCTTCCAATAG TCCTGGTCAGCCTAATTCTGTGAAGAGAAAGAAACTACCTGTAGATAGTGTCTTTAACAAATTTGAGGAT GAAGACAGTGATGACGTACCCCGAAAAAGGAAACTGGTTCCCTTGGATTATGGTGAAGATGATAAAAATG CAACCAAAGGCACTGTAAACACTGAAGAAAAGCGTAAACACATTAAGAGTCTCATTGAGAAAATCCCTAC AGCCAAACCTGAGCTCTTCGCTTATCCCCTGGATTGGTCTATTGTGGATTCTATACTGATGGAACGTCGA ATTAGACCATGGATTAATAAGAAAATCATAGAATATATAGGTGAAGAAGAAGCTACATTAGTTGATTTTG TTTGTTCTAAGGTTATGGCTCATAGTTCACCCCAGAGCATTTTAGATGATGTTGCCATGGTACTTGATGA AGAAGCAGAAGTTTTTATAGTCAAAATGTGGAGATTATTGATATATGAAACAGAAGCCAAGAAAATTGGT CTTGTGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212256 representing NM_021239
Red=Cloning site Green=Tags(s) MSFPPHLNRPPMGIPALPPGIPPPQFPGFPPPVPPGTPMIPVPMSIMAPAPTVLVPTVSMVGKHLGARKD HPGLKAKENDENCGPTTTVFVGNISEKASDMLIRQLLAKCGLVLSWKRVQGASGKLQAFGFCEYKEPEST LRALRLLHDLQIGEKKLLVKVDAKTKAQLDEWKAKKKASNGNARPETVTNDDEEALDEETKRRDQMIKGA IEVLIREYSSELNAPSQESDSHPRKKKKEKKEDIFRRFPVAPLIPYPLITKEDINAIEMEEDKRDLISRE ISKFRDTHKKLEEEKGKKEKERQEIEKERRERERERERERERREREREREREREREKEKERERERERDRD RDRTKERDRDRDRERDRDRDRERSSDRNKDRSRSREKSRDRERERERERERERERERERERERERERERE REREREKDKKRDREEDEEDAYERRKLERKLREKEAAYQERLKNWEIRERKKTREYEKEAEREEERRREMA KEAKRLKEFLEDYDDDRDDPKYYRGSALQKRLRDREKEMEADERDRKREKEELEEIRQRLLAEGHPDPDA ELQRMEQEAERRRQPQIKQEPESEEEEEEKQEKEEKREEPMEEEEEPEQKPCLKPTLRPISSAPSVSSAS GNATPNTPGDESPCGIIIPHENSPDQQQPEEHRPKIGLSLKLGASNSPGQPNSVKRKKLPVDSVFNKFED EDSDDVPRKRKLVPLDYGEDDKNATKGTVNTEEKRKHIKSLIEKIPTAKPELFAYPLDWSIVDSILMERR IRPWINKKIIEYIGEEEATLVDFVCSKVMAHSSPQSILDDVAMVLDEEAEVFIVKMWRLLIYETEAKKIG LVK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021239 |
| ORF Size | 2529 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021239.1, NP_067062.1 |
| RefSeq Size | 4189 bp |
| RefSeq ORF | 2532 bp |
| Locus ID | 58517 |
| UniProt ID | P49756 |
| Protein Pathways | Spliceosome |
| MW | 100 kDa |
| Gene Summary | RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
RNA-binding proteins with basic-acidic dipeptide (BAD) domains self-assemble and aggregate in Alzheimer's disease
,Bishof, I;Dammer, EB;Duong, DM;Kundinger, S;Gearing, M;Lah, JJ;Levey, AI;Seyfried, NT;,
J. Biol. Chem.
,PubMed ID 29802200
[RBM25]
|
|
Role of RBM25/LUC7L3 in Abnormal Cardiac Sodium Channel Splicing Regulation in Human Heart Failure
,Ge Gao, An Xie, Shu-Ching Huang, Anyu Zhou, Jianhua Zhang, Amanda M. Herman, Sassan Ghassemzadeh, Euy-Myoung Jeong, Srinivasan Kasturirangan, Mihai Raicu, Michael A. Sobieski, II, Geetha Bhat, Antone Tatooles, Edward J. Benz, Jr, Timothy J. Kamp, and Samuel C. Dudley, Jr,
Circulation, Sep 2011; 124: 1124 - 1131.
[RBM25]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212256L3 | Lenti-ORF clone of RBM25 (Myc-DDK-tagged)-Human RNA binding motif protein 25 (RBM25) |
CNY 9,030.00 |
|
| RC212256L4 | Lenti-ORF clone of RBM25 (mGFP-tagged)-Human RNA binding motif protein 25 (RBM25) |
CNY 9,030.00 |
|
| RG212256 | RBM25 (tGFP-tagged) - Human RNA binding motif protein 25 (RBM25) |
CNY 10,864.00 |
|
| SC304929 | RBM25 (untagged)-Human RNA binding motif protein 25 (RBM25) |
CNY 6,184.00 |
