MYH14 (NM_024729) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DFNA4; DFNA4A; FP17425; MHC16; MYH17; myosin; NMHC-II-C; NMHC II-C; PNMHH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC211638 representing NM_024729 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCGTGACCATGTCGGTGCCCGGGCGGAAGGCGCCCCCCAGGCCGGGCCCAGTGCCCGAGGCGG CCCAGCCGTTCCTGTTCACGCCCCGCGGGCCCAGCGCGGGTGGCGGGCCTGGCTCGGGCACCTCCCCGCA GGTGGAGTGGACGGCCCGGCGTCTCGTGTGGGTGCCTTCGGAGCTTCACGGGTTCGAGGCGGCGGCGCTG CGGGACGAAGGCGAGGAGGAGGCGGAGGTGGAGCTGGCGGAGAGCGGGAGGCGGCTGCGACTGCCGCGGG ACCAGATCCAGCGCATGAACCCGCCCAAGTTCAGCAAGGCCGAGGACATGGCCGAGCTGACCTGCCTCAA CGAGGCCTCGGTCCTGCACAACCTCCGGGAGCGGTACTACTCCGGCCTCATCTACACGTACTCCGGCCTT TTCTGTGTGGTCATCAACCCGTACAAGCAGCTTCCCATCTACACAGAAGCCATTGTGGAGATGTACCGGG GCAAGAAGCGCCACGAGGTGCCACCCCACGTGTACGCAGTGACCGAGGGGGCCTATCGGAGCATGCTGCA GGATCGTGAGGACCAGTCCATTCTCTGCACTGGAGAGTCTGGAGCTGGGAAGACGGAAAACACCAAGAAG GTCATCCAGTACCTCGCCCACGTGGCGTCGTCTCCAAAGGGCAGGAAGGAGCCGGGTGTCCCCGGTGAGC TGGAGCGGCAGCTGCTTCAGGCCAACCCCATCCTAGAGGCCTTTGGCAATGCCAAGACAGTGAAGAATGA CAACTCCTCCCGATTCGGCAAATTCATCCGCATCAACTTTGATGTTGCCGGGTACATCGTGGGCGCCAAC ATTGAGACCTACCTGCTGGAGAAGTCGCGGGCCATCCGCCAGGCCAAGGACGAGTGCAGCTTCCACATCT TCTACCAGCTGCTGGGGGGCGCTGGAGAGCAGCTCAAAGCCGACCTCCTCCTCGAGCCCTGCTCCCACTA CCGGTTCCTGACCAACGGGCCGTCATCCTCTCCCGGCCAGGAGCGGGAACTCTTCCAGGAGACGCTGGAG TCGCTGCGGGTCCTGGGATTCAGCCACGAGGAAATCATCTCCATGCTGCGGATGGTCTCAGCAGTTCTCC AGTTTGGCAACATTGCCTTGAAGAGAGAACGGAACACCGATCAAGCCACCATGCCTGACAACACAGCTGC ACAGAAGCTCTGCCGCCTCTTGGGACTGGGGGTGACGGATTTCTCCCGAGCCTTGCTCACCCCTCGCATC AAAGTTGGCCGAGACTATGTGCAGAAAGCCCAGACTAAGGAACAGGCTGACTTCGCGCTGGAGGCCCTGG CCAAGGCCACCTACGAGCGCCTCTTCCGCTGGCTGGTTCTGCGCCTCAACCGGGCCTTGGACCGCAGCCC CCGCCAAGGCGCCTCCTTCCTGGGCATCCTGGACATCGCGGGCTTTGAGATCTTCCAGCTGAACTCCTTC GAGCAGCTCTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTCTTCAACCACACCATGTTCGTGCTGG AGCAGGAGGAGTACCAGCGTGAGGGCATCCCCTGGACCTTCCTCGACTTTGGCCTCGACCTGCAGCCCTG CATCGACCTCATCGAGCGGCCGGCCAACCCCCCTGGACTCCTGGCCCTGCTGGATGAGGAGTGCTGGTTC CCGAAGGCCACAGACAAGTCGTTTGTGGAGAAGGTAGCCCAGGAGCAGGGCGGCCACCCCAAGTTCCAGC GGCCGAGGCACCTGCGGGATCAGGCCGACTTCAGTGTTCTCCACTACGCGGGCAAGGTCGACTACAAGGC CAACGAGTGGCTGATGAAAAACATGGACCCTCTGAATGACAACGTCGCAGCCTTGCTCCACCAGAGCACA GACCGGCTGACGGCAGAGATCTGGAAAGACGTGGAGGGCATCGTGGGGCTGGAACAGGTGAGCAGCCTGG GCGACGGCCCACCAGGTGGCCGCCCCCGTCGGGGTATGTTCCGGACAGTGGGACAGCTCTACAAGGAGTC CCTGAGCCGCCTCATGGCCACACTCAGCAACACCAACCCCAGTTTTGTCCGCTGCATTGTCCCCAACCAC GAGAAGAGGGCCGGGAAGCTGGAGCCACGGCTGGTGCTGGACCAGCTTCGCTGCAACGGGGTCCTGGAGG GCATCCGCATCTGTCGCCAGGGCTTCCCCAACCGCATCCTCTTCCAGGAGTTCCGGCAGCGATACGAGAT CCTGACACCCAATGCCATCCCCAAGGGCTTCATGGATGGGAAGCAGGCCTGTGAAAAGATGATCCAGGCG CTGGAACTGGACCCCAACCTCTACCGCGTGGGACAGAGCAAGATCTTCTTCCGGGCTGGGGTCCTGGCCC AGCTGGAAGAGGAGCGAGACCTGAAGGTCACCGACATCATCGTCTCCTTCCAGGCAGCTGCCCGGGGATA CCTGGCTCGCAGGGCCTTCCAGAAGCGCCAGCAGCAGCAGAGCGCCCTGAGGGTGATGCAGCGGAACTGC GCGGCCTACCTCAAGCTGAGACACTGGCAGTGGTGGCGGCTGTTTACCAAGGTGAAGCCACTGCTGCAGG TGACGCGGCAGGATGAGGTGCTGCAGGCACGGGCCCAGGAGCTGCAGAAAGTGCAGGAGCTACAGCAGCA GAGCGCCCGCGAAGTTGGGGAGCTCCAGGGCCGAGTGGCACAGCTGGAAGAGGAGCGCGCCCGCCTGGCA GAGCAATTGCGAGCAGAGGCAGAACTGTGTGCAGAGGCCGAGGAGACGCGGGGGAGGCTGGCAGCCCGCA AGCAGGAGCTGGAGCTGGTGGTGTCAGAGCTGGAGGCTCGCGTGGGCGAGGAGGAGGAGTGCAGCCGTCA AATGCAAACCGAGAAGAAGAGGCTGCAGCAGCACATACAGGAGCTAGAGGCCCACCTTGAGGCTGAGGAG GGTGCGCGGCAGAAGCTGCAGCTGGAGAAGGTGACGACAGAGGCAAAAATGAAGAAATTTGAAGAGGACC TGCTGCTCCTGGAAGACCAGAATTCCAAGCTGAGCAAGGAGCGGAAGCTGCTGGAAGATCGTCTGGCCGA GTTCTCATCCCAGGCAGCTGAGGAGGAGGAGAAGGTCAAGAGCCTCAATAAGCTACGGCTCAAATATGAG GCCACAATCGCAGACATGGAGGACCGCCTACGGAAGGAGGAGAAGGGTCGCCAGGAGCTGGAGAAGCTGA AGCGGAGGCTGGATGGGGAGAGCTCAGAGCTGCAGGAGCAGATGGTGGAGCAGCAACAGCGGGCAGAGGA GCTGCGGGCCCAGCTGGGCCGGAAGGAGGAGGAGCTGCAGGCTGCCCTGGCCAGGGCAGAAGACGAGGGT GGGGCCCGGGCCCAGCTGCTGAAATCCCTGCGGGAGGCTCAAGCAGCCCTGGCCGAGGCCCAGGAGGACC TGGAGTCTGAGCGTGTGGCCAGGACCAAGGCGGAGAAGCAGCGCCGGGACCTGGGCGAGGAGCTGGAGGC GCTGCGGGGCGAGCTGGAGGACACGCTGGACTCCACCAACGCACAGCAGGAGCTCCGGTCCAAGAGGGAA CAGGAGGTGACGGAGCTGAAGAAGACTCTGGAGGAGGAGACTCGCATCCACGAGGCGGCAGTGCAGGAGC TGAGGCAGCGCCACGGCCAGGCCCTGGGGGAGCTGGCGGAGCAGCTGGAGCAGGCCCGGAGGGGCAAAGG TGCATGGGAGAAGACCCGGCTGGCCCTGGAGGCCGAGGTGTCCGAGCTGCGGGCAGAACTGAGCAGCCTG CAGACTGCACGTCAGGAGGGTGAGCAGCGGAGGCGCCGCCTGGAGTTACAGCTGCAGGAGGTGCAGGGCC GGGCTGGTGATGGGGAGAGGGCACGAGCGGAGGCTGCTGAGAAGCTGCAGCGAGCCCAGGCTGAACTGGA GAATGTGTCTGGGGCGCTGAACGAGGCTGAGTCCAAAACCATCCGTCTTAGCAAGGAGCTGAGCAGCACA GAAGCCCAGCTGCACGATGCCCAGGAGCTGCTGCAGGAGGAGACCAGGGCGAAATTGGCCTTGGGGTCCC GGGTGCGAGCCATGGAGGCTGAGGCAGCCGGGCTGCGTGAGCAGCTGGAGGAGGAGGCAGCTGCCAGGGA ACGGGCGGGCCGTGAACTGCAGACTGCCCAGGCCCAGCTTTCCGAGTGGCGGCGGCGCCAGGAGGAGGAG GCAGGGGCACTGGAGGCAGGGGAGGAGGCACGGCGCCGGGCAGCCCGGGAGGCCGAGGCCCTGACCCAGC GCCTGGCAGAAAAGACAGAGACCGTGGATCGGCTGGAGCGGGGCCGCCGCCGGCTGCAGCAGGAGCTGGA CGACGCCACCATGGACCTGGAGCAGCAGCGGCAGCTTGTGAGCACCCTGGAGAAGAAGCAGCGCAAGTTT GACCAGCTTCTGGCAGAGGAGAAGGCAGCTGTACTTCGGGCAGTGGAGGAACGTGAGCGGGCCGAGGCAG AGGGCCGGGAGCGTGAGGCTCGGGCCCTGTCACTGACACGGGCACTGGAGGAGGAGCAGGAGGCACGTGA GGAGCTGGAGCGGCAGAACCGGGCCCTGCGGGCTGAGCTGGAGGCACTGCTGAGCAGCAAGGATGACGTC GGCAAGAGCGTGCATGAGCTGGAACGAGCCTGCCGGGTAGCAGAACAGGCAGCCAATGATCTGCGAGCAC AGGTGACAGAACTGGAGGATGAGCTGACAGCGGCCGAGGATGCCAAGCTGCGTCTGGAGGTGACTGTGCA GGCTCTCAAGACTCAGCATGAGCGTGACCTGCAGGGCCGTGATGAGGCTGGTGAAGAGAGGCGGAGGCAG CTGGCCAAGCAGCTGAGAGATGCAGAGGTGGAGCGGGATGAGGAGCGGAAGCAGCGCACTCTGGCCGTGG CTGCCCGCAAGAAGCTGGAGGGAGAGCTGGAGGAGCTGAAGGCTCAGATGGCCTCTGCCGGCCAGGGCAA GGAGGAGGCGGTGAAGCAGCTTCGCAAGATGCAGGCCCAGATGAAGGAGCTATGGCGGGAGGTGGAGGAG ACACGCACCTCCCGGGAGGAGATCTTCTCCCAGAATCGGGAAAGTGAAAAGCGCCTCAAGGGCCTGGAGG CTGAGGTGCTGCGGCTGCAGGAGGAACTGGCCGCCTCGGACCGTGCTCGGCGGCAGGCCCAGCAGGACCG GGATGAGATGGCAGATGAGGTGGCCAATGGTAACCTTAGCAAGGCAGCCATTCTGGAGGAGAAGCGTCAG CTGGAGGGGCGCCTGGGGCAGTTGGAGGAAGAGCTGGAGGAGGAGCAGAGCAACTCGGAGCTGCTCAATG ACCGCTACCGCAAGCTGCTCCTGCAGGTAGAGTCACTGACCACAGAGCTGTCAGCTGAGCGCAGTTTCTC AGCCAAGGCAGAGAGCGGGCGGCAGCAGCTGGAACGGCAGATCCAGGAGCTACGGGGACGCCTGGGTGAG GAGGATGCTGGGGCCCGTGCCCGCCACAAGATGACCATTGCTGCCCTTGAGTCTAAGTTGGCCCAGGCTG AGGAGCAGCTAGAGCAAGAGACCAGAGAGCGCATCCTCTCTGGAAAGCTGGTGCGCAGAGCTGAGAAGCG GCTTAAAGAGGTGGTGCTCCAGGTGGAGGAGGAGCGGAGGGTGGCTGACCAGCTCCGGGACCAGCTGGAG AAGGGAAACCTTCGAGTCAAGCAGCTGAAGCGGCAGCTGGAGGAGGCCGAGGAGGAGGCATCCCGGGCTC AGGCCGGCCGCCGGAGGCTGCAGCGTGAGCTGGAAGATGTCACAGAGTCGGCCGAGTCCATGAACCGTGA AGTGACCACACTGAGGAACCGGCTTCGACGCGGCCCCCTCACCTTCACCACCCGCACGGTGCGCCAGGTC TTCCGACTAGAGGAGGGCGTGGCATCCGACGAGGAGGCAGAGGAAGCACAGCCTGGGTCTGGGCCATCCC CGGAGCCTGAGGGGTCCCCACCAGCCCACCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC211638 representing NM_024729 Red=Cloning site Green=Tags(s) MAAVTMSVPGRKAPPRPGPVPEAAQPFLFTPRGPSAGGGPGSGTSPQVEWTARRLVWVPSELHGFEAAAL RDEGEEEAEVELAESGRRLRLPRDQIQRMNPPKFSKAEDMAELTCLNEASVLHNLRERYYSGLIYTYSGL FCVVINPYKQLPIYTEAIVEMYRGKKRHEVPPHVYAVTEGAYRSMLQDREDQSILCTGESGAGKTENTKK VIQYLAHVASSPKGRKEPGVPGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGAN IETYLLEKSRAIRQAKDECSFHIFYQLLGGAGEQLKADLLLEPCSHYRFLTNGPSSSPGQERELFQETLE SLRVLGFSHEEIISMLRMVSAVLQFGNIALKRERNTDQATMPDNTAAQKLCRLLGLGVTDFSRALLTPRI KVGRDYVQKAQTKEQADFALEALAKATYERLFRWLVLRLNRALDRSPRQGASFLGILDIAGFEIFQLNSF EQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIPWTFLDFGLDLQPCIDLIERPANPPGLLALLDEECWF PKATDKSFVEKVAQEQGGHPKFQRPRHLRDQADFSVLHYAGKVDYKANEWLMKNMDPLNDNVAALLHQST DRLTAEIWKDVEGIVGLEQVSSLGDGPPGGRPRRGMFRTVGQLYKESLSRLMATLSNTNPSFVRCIVPNH EKRAGKLEPRLVLDQLRCNGVLEGIRICRQGFPNRILFQEFRQRYEILTPNAIPKGFMDGKQACEKMIQA LELDPNLYRVGQSKIFFRAGVLAQLEEERDLKVTDIIVSFQAAARGYLARRAFQKRQQQQSALRVMQRNC AAYLKLRHWQWWRLFTKVKPLLQVTRQDEVLQARAQELQKVQELQQQSAREVGELQGRVAQLEEERARLA EQLRAEAELCAEAEETRGRLAARKQELELVVSELEARVGEEEECSRQMQTEKKRLQQHIQELEAHLEAEE GARQKLQLEKVTTEAKMKKFEEDLLLLEDQNSKLSKERKLLEDRLAEFSSQAAEEEEKVKSLNKLRLKYE ATIADMEDRLRKEEKGRQELEKLKRRLDGESSELQEQMVEQQQRAEELRAQLGRKEEELQAALARAEDEG GARAQLLKSLREAQAALAEAQEDLESERVARTKAEKQRRDLGEELEALRGELEDTLDSTNAQQELRSKRE QEVTELKKTLEEETRIHEAAVQELRQRHGQALGELAEQLEQARRGKGAWEKTRLALEAEVSELRAELSSL QTARQEGEQRRRRLELQLQEVQGRAGDGERARAEAAEKLQRAQAELENVSGALNEAESKTIRLSKELSST EAQLHDAQELLQEETRAKLALGSRVRAMEAEAAGLREQLEEEAAARERAGRELQTAQAQLSEWRRRQEEE AGALEAGEEARRRAAREAEALTQRLAEKTETVDRLERGRRRLQQELDDATMDLEQQRQLVSTLEKKQRKF DQLLAEEKAAVLRAVEERERAEAEGREREARALSLTRALEEEQEAREELERQNRALRAELEALLSSKDDV GKSVHELERACRVAEQAANDLRAQVTELEDELTAAEDAKLRLEVTVQALKTQHERDLQGRDEAGEERRRQ LAKQLRDAEVERDEERKQRTLAVAARKKLEGELEELKAQMASAGQGKEEAVKQLRKMQAQMKELWREVEE TRTSREEIFSQNRESEKRLKGLEAEVLRLQEELAASDRARRQAQQDRDEMADEVANGNLSKAAILEEKRQ LEGRLGQLEEELEEEQSNSELLNDRYRKLLLQVESLTTELSAERSFSAKAESGRQQLERQIQELRGRLGE EDAGARARHKMTIAALESKLAQAEEQLEQETRERILSGKLVRRAEKRLKEVVLQVEEERRVADQLRDQLE KGNLRVKQLKRQLEEAEEEASRAQAGRRRLQRELEDVTESAESMNREVTTLRNRLRRGPLTFTTRTVRQV FRLEEGVASDEEAEEAQPGSGPSPEPEGSPPAHPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_024729
ORF Size	5985 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_024729.3, NP_079005.3
RefSeq Size	6807 bp
RefSeq ORF	5988 bp
Locus ID	79784
UniProt ID	Q7Z406
Domains	Myosin_tail, M
Protein Pathways	Regulation of actin cytoskeleton, Tight junction, Viral myocarditis
MW	227.9 kDa
Gene Summary	This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-14 (MYO14). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene result in one form of autosomal dominant hearing impairment. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

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