United States
Germany
Japan
United Kingdom
China
BRF1 (NM_145685) Human Tagged ORF Clone
CAT＃: RC210551
- TrueORF®
BRF1 (Myc-DDK-tagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3
ORF Plasmid: tGFP
"NM_145685" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p; TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210551 representing NM_145685
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGGCCGTGTGTGCCGCGGTTGCGGCGGCACGGACATCGAGCTGGACGCGGCGCGCGGGGACGCGG TGTGCACCGCCTGCGGCTCAGTGCTGGAGGACAACATCATCGTGTCCGAGGTGCAGTTCGTGGAGAGCAG CGGCGGCGGCTCCTCGGCCGTGGGCCAGTTCGTGTCCCTGGACGGGAGGCGCCACATCCACCACCTGGGG AACCAGCTGCAGCTGAACCAGCACTGCCTGGACACCGCCTTCAACTTCTTCAAGATGGCCGTGAGCAGGC ACCTGACCCGCGGCCGGAAGATGGCCCACGTGATTGCTGCCTGCCTCTACCTGGTCTGCCGTACGGAGGG CACGCCGCACATGCTCCTGGACCTCAGCGACCTGCTCCAGGTGAATGTGTACGTGCTTGGAAAGACGTTT CTTCTCTTGGCAAGAGAGCTCTGCATCAATGCGCCGGCCATAGACCCGTGCCTGTATATTCCACGCTTTG CGCACCTGCTGGAATTCGGGGAGAAGAACCACGAGGTGTCCATGACTGCCCTGAGGCTCCTACAGAGGAT GAAGCGGGACTGGATGCACACAGGCCGGCGCCCCTCGGGCCTCTGCGGAGCAGCGCTCCTGGTTGCAGCC AGAATGCATGACTTCAGGAGGACTGTGAAGGAGGTCATCAGTGTGGTCAAAGTGTGTGAGTCCACGCTGC GGAAGAGGCTCACGGAATTTGAAGACACCCCCACCAGTCAGTTGACCATTGATGAGTTCATGAAGATCGA CCTGGAGGAGGAGTGCGACCCCCCCTCGTACACAGCTGGGCAGAGGAAGCTGCGGATGAAGCAGCTTGAA CAAGTCCTGTCAAAAAAACTGGAGGAGGTTGAAGGTGAAATATCCAGTTACCAGGATGCAATTGAGATTG AACTAGAAAACAGCCGGCCAAAGGCCAAGGGGGGCCTGGCCAGCCTGGCAAAAGATGGCTCCACCGAGGA CACCGCGTCCAGCTTGTGTGGCGAGGAGGACACAGAGGACGAGGAGCTGGAAGCCGCGGCCAGCCACCTG AACAAAGACTTATACCGGGAGCTCCTTGGTGGTGCCCCCGGCAGCTCGGAAGCAGCAGGAAGCCCCGAGT GGGGCGGCAGACCTCCGGCCCTGGGGTCCCTGCTGGACCCCCTCCCCACTGCAGCCAGCCTGGGCATCTC AGACTCCATCCGCGAATGCATCTCCTCTCAGAGCAGCGACCCCAAAGATGCTTCAGGAGACGGTGAGCTG GACCTCAGTGGCATTGATGACCTGGAGATTGACAGGTACATCCTGAATGAGTCGGAAGCCCGCGTGAAGG CCGAGCTGTGGATGAGGGAGAACGCCGAGTACCTGCGGGAACAGAGGGAAAAAGAAGCAAGAATAGCGAA AGAGAAGGAGCTCGGCATCTACAAGGAACACAAGCCCAAGAAGTCTTGCAAGCGACGGGAGCCAATTCAG GCCAGTACCGCCAGGGAGGCCATCGAGAAGATGCTGGAGCAGAAGAAGATCTCCAGCAAGATCAATTATA GCGTGCTCCGGGGCCTCAGCAGCGCCGGCGGGGGCAGTCCGCACAGGGAGGATGCACAGCCCGAGCATAG CGCCAGTGCCAGGAAGCTGTCACGAAGGAGGACGCCGGCCAGCAGAAGTGGGGCTGACCCTGTGACCAGT GTGGGGAAAAGGTTGAGGCCTCTGGTGTCTACGCAGCCAGCAAAGAAGGTGGCCACGGGAGAGGCTTTGC TCCCAAGCTCTCCCACCCTCGGAGCTGAGCCTGCCAGGCCCCAGGCGGTGCTGGTGGAGAGCGGGCCCGT GTCATACCACGCCGACGAGGAGGCTGACGAGGAGGAGCCTGACGAGGAGGACGGGGAGCCCTGCGTCAGT GCCCTGCAGATGATGGGCAGCAACGACTATGGCTGTGATGGCGATGAGGACGACGGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210551 representing NM_145685
Red=Cloning site Green=Tags(s) MTGRVCRGCGGTDIELDAARGDAVCTACGSVLEDNIIVSEVQFVESSGGGSSAVGQFVSLDGRRHIHHLG NQLQLNQHCLDTAFNFFKMAVSRHLTRGRKMAHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYVLGKTF LLLARELCINAPAIDPCLYIPRFAHLLEFGEKNHEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLVAA RMHDFRRTVKEVISVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEEECDPPSYTAGQRKLRMKQLE QVLSKKLEEVEGEISSYQDAIEIELENSRPKAKGGLASLAKDGSTEDTASSLCGEEDTEDEELEAAASHL NKDLYRELLGGAPGSSEAAGSPEWGGRPPALGSLLDPLPTAASLGISDSIRECISSQSSDPKDASGDGEL DLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQREKEARIAKEKELGIYKEHKPKKSCKRREPIQ ASTAREAIEKMLEQKKISSKINYSVLRGLSSAGGGSPHREDAQPEHSASARKLSRRRTPASRSGADPVTS VGKRLRPLVSTQPAKKVATGEALLPSSPTLGAEPARPQAVLVESGPVSYHADEEADEEEPDEEDGEPCVS ALQMMGSNDYGCDGDEDDGY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145685 |
| ORF Size | 1953 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145685.1, NM_145685.2, NP_663718.1 |
| RefSeq Size | 3585 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 2972 |
| UniProt ID | Q92994 |
| Domains | transcript_fac2 |
| Protein Families | Transcription Factors |
| MW | 71.1 kDa |
| Gene Summary | This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210551L3 | Lenti-ORF clone of BRF1 (Myc-DDK-tagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3 |
CNY 5,890.00 |
|
| RC210551L4 | Lenti-ORF clone of BRF1 (mGFP-tagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3 |
CNY 5,890.00 |
|
| RG210551 | BRF1 (tGFP-tagged) - Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3 |
CNY 4,370.00 |
|
| SC108221 | BRF1 (untagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3 |
CNY 8,320.00 |
