P2Y11 (P2RY11) (NM_002566) Human Tagged ORF Clone

CAT#: RC210061

  • TrueORF®

P2RY11 (Myc-DDK-tagged)-Human purinergic receptor P2Y, G-protein coupled, 11 (P2RY11)

ORF Plasmid: DDK tGFP



  "NM_002566" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


P2RY11 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms P2Y11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210061 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCAACGTCTCGGGTGCCAAGTCCTGCCCTGCCAACTTCTTGGCAGCTGCCGACGACAAACTCA
GTGGGTTCCAGGGGGACTTCCTGTGGCCCATACTGGTGGTTGAGTTCCTGGTGGCCGTGGCCAGCAATGG
CCTGGCCCTGTACCGCTTCAGCATCCGGAAGCAGCGCCCATGGCACCCCGCCGTGGTCTTCTCTGTCCAG
CTGGCAGTCAGCGACCTGCTCTGCGCTCTGACGCTGCCCCCGCTGGCCGCCTACCTCTATCCCCCCAAGC
ACTGGCGCTATGGGGAGGCCGCGTGCCGCCTGGAGCGCTTCCTCTTCACCTGCAACCTGCTGGGCAGCGT
CATCTTCATCACCTGCATCAGCCTCAACCGCTACCTGGGCATCGTGCACCCCTTCTTCGCCCGAAGCCAC
CTGCGACCCAAGCACGCCTGGGCCGTGAGCGCTGCCGGCTGGGTCCTGGCCGCCCTGCTGGCCATGCCCA
CACTCAGCTTCTCCCACCTGAAGAGGCCGCAGCAGGGGGCGGGCAACTGCAGCGTGGCCAGGCCCGAGGC
CTGCATCAAGTGTCTGGGGACAGCAGACCACGGGCTGGCGGCCTACAGAGCGTATAGCCTGGTGCTGGCG
GGGTTGGGCTGCGGCCTGCCGCTGCTGCTCACGCTGGCAGCCTACGGCGCCCTCGGGCGGGCCGTGCTAC
GCAGCCCAGGCATGACTGTGGCCGAGAAGCTGCGTGTGGCAGCGTTGGTGGCCAGTGGTGTGGCCCTCTA
CGCCAGCTCCTATGTGCCCTACCACATCATGCGGGTGCTCAACGTGGATGCTCGGCGGCGCTGGAGCACC
CGCTGCCCGAGCTTTGCAGACATAGCCCAGGCCACAGCAGCCCTGGAGCTGGGGCCCTACGTGGGCTACC
AGGTGATGCGGGGCCTCATGCCCCTGGCCTTCTGTGTCCACCCTCTACTCTACATGGCCGCAGTGCCCAG
CCTGGGCTGCTGCTGCCGACACTGCCCCGGCTACAGGGACAGCTGGAACCCAGAGGACGCCAAGAGCACT
GGCCAAGCCCTGCCCCTCAATGCCACAGCCGCCCCTAAACCGTCAGAGCCCCAGTCCCGTGAGCTGAGCC
AA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210061 protein sequence
Red=Cloning site Green=Tags(s)

MAANVSGAKSCPANFLAAADDKLSGFQGDFLWPILVVEFLVAVASNGLALYRFSIRKQRPWHPAVVFSVQ
LAVSDLLCALTLPPLAAYLYPPKHWRYGEAACRLERFLFTCNLLGSVIFITCISLNRYLGIVHPFFARSH
LRPKHAWAVSAAGWVLAALLAMPTLSFSHLKRPQQGAGNCSVARPEACIKCLGTADHGLAAYRAYSLVLA
GLGCGLPLLLTLAAYGALGRAVLRSPGMTVAEKLRVAALVASGVALYASSYVPYHIMRVLNVDARRRWST
RCPSFADIAQATAALELGPYVGYQVMRGLMPLAFCVHPLLYMAAVPSLGCCCRHCPGYRDSWNPEDAKST
GQALPLNATAAPKPSEPQSRELSQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002566
ORF Size 1122 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002566.2
RefSeq Size 1977 bp
RefSeq ORF 1125 bp
Locus ID 5032
UniProt ID Q96G91
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 40.3 kDa
Gene Summary The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is coupled to the stimulation of the phosphoinositide and adenylyl cyclase pathways and behaves as a selective purinoceptor. Naturally occuring read-through transcripts, resulting from intergenic splicing between this gene and an immediately upstream gene (PPAN, encoding peter pan homolog), have been found. The PPAN-P2RY11 read-through mRNA is ubiquitously expressed and encodes a fusion protein that shares identity with each individual gene product. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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