NPBWR2 (NM_005286) Human Tagged ORF Clone

CAT#: RC209677

NPBWR2 (Myc-DDK-tagged)-Human neuropeptides B/W receptor 2 (NPBWR2)

ORF Plasmid: DDK tGFP



  "NM_005286" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


NPBWR2 / GPR8 Rabbit Polyclonal (N-Terminus) Antibody
    • 50 ug

CNY 5,324.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms GPR8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209677 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCCGCTGGGCACCCAGAGCCCCTTGACAGCAGGGGCTCCTTCTCCCTCCCCACGATGGGTGCCA
ACGTCTCTCAGGACAATGGCACTGGCCACAATGCCACCTTCTCCGAGCCACTGCCGTTCCTCTATGTGCT
CCTGCCCGCCGTGTACTCCGGGATCTGTGCTGTGGGGCTGACTGGCAACACGGCCGTCATCCTTGTAATC
CTAAGGGCGCCCAAGATGAAGACGGTGACCAACGTGTTCATCCTGAACCTGGCCGTCGCCGACGGGCTCT
TCACGCTGGTACTGCCTGTCAACATCGCGGAGCACCTGCTGCAGTACTGGCCCTTCGGGGAGCTGCTCTG
CAAGCTGGTGCTGGCCGTCGACCACTACAACATCTTCTCCAGCATCTACTTCCTAGCCGTGATGAGCGTG
GACCGATACCTGGTGGTGCTGGCCACCGTGAGGTCCCGCCACATGCCCTGGCGCACCTACCGGGGGGCGA
AGGTCGCCAGCCTGTGTGTCTGGCTGGGCGTCACGGTCCTGGTTCTGCCCTTCTTCTCTTTCGCTGGCGT
CTACAGCAACGAGCTGCAGGTCCCAAGCTGTGGGCTGAGCTTCCCGTGGCCCGAGCAGGTCTGGTTCAAG
GCCAGCCGTGTCTACACGTTGGTCCTGGGCTTCGTGCTGCCCGTGTGCACCATCTGTGTGCTCTACACAG
ACCTCCTGCGCAGGCTGCGGGCCGTGCGGCTCCGCTCTGGAGCCAAGGCTCTAGGCAAGGCCAGGCGGAA
GGTGACCGTCCTGGTCCTCGTCGTGCTGGCCGTGTGCCTCCTCTGCTGGACGCCCTTCCACCTGGCCTCT
GTCGTGGCCCTGACCACGGACCTGCCCCAGACCCCACTGGTCATCAGTATGTCCTACGTCATCACCAGCC
TCAGCTACGCCAACTCGTGCCTGAACCCCTTCCTCTACGCCTTTCTAGATGACAACTTCCGGAAGAACTT
CCGCAGCATATTGCGGTGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209677 protein sequence
Red=Cloning site Green=Tags(s)

MQAAGHPEPLDSRGSFSLPTMGANVSQDNGTGHNATFSEPLPFLYVLLPAVYSGICAVGLTGNTAVILVI
LRAPKMKTVTNVFILNLAVADGLFTLVLPVNIAEHLLQYWPFGELLCKLVLAVDHYNIFSSIYFLAVMSV
DRYLVVLATVRSRHMPWRTYRGAKVASLCVWLGVTVLVLPFFSFAGVYSNELQVPSCGLSFPWPEQVWFK
ASRVYTLVLGFVLPVCTICVLYTDLLRRLRAVRLRSGAKALGKARRKVTVLVLVVLAVCLLCWTPFHLAS
VVALTTDLPQTPLVISMSYVITSLSYANSCLNPFLYAFLDDNFRKNFRSILRC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005286
ORF Size 1001 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005286.4
RefSeq Size 1002 bp
RefSeq ORF 1002 bp
Locus ID 2832
UniProt ID P48146
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 36.9 kDa
Gene Summary The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor. The encoded protein is similar in sequence to another G protein-coupled receptor (GPR7), and it is structurally similar to opioid and somatostatin receptors. This protein binds neuropeptides B and W. This gene is intronless and is expressed primarily in the frontal cortex of the brain. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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