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GPR116 (ADGRF5) (NM_015234) Human Tagged ORF Clone
CAT＃: RC209170
GPR116 (Myc-DDK-tagged)-Human G protein-coupled receptor 116 (GPR116), transcript variant 1
ORF Plasmid: tGFP
"NM_015234" in other vectors (6)
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CNY 13,824.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPR116; KPG_001 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209170 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCCCCAAGGAGAACCACTTTGTGCCTCATGTTTATTGTGATTTATTCTTCCAAAGCTGCACTGA ACTGGAATTACGAGTCTACTATTCATCCTTTGAGTCTTCATGAACATGAACCAGCTGGTGAAGAGGCACT GAGGCAAAAACGAGCCGTTGCCACAAAAAGTCCTACGGCTGAAGAATACACTGTTAATATTGAGATCAGT TTTGAAAATGCATCCTTCCTGGATCCTATCAAAGCCTACTTGAACAGCCTCAGTTTTCCAATTCATGGGA ATAACACTGACCAAATTACCGACATTTTGAGCATAAATGTGACAACAGTCTGCAGACCTGCTGGAAATGA AATCTGGTGCTCCTGCGAGACAGGTTATGGGTGGCCTCGGGAAAGGTGTCTTCACAATCTCATTTGTCAA GAGCGTGACGTCTTCCTCCCAGGGCACCATTGCAGTTGCCTTAAAGAACTGCCTCCCAATGGACCTTTTT GCCTGCTTCAGGAAGATGTTACCCTGAACATGAGAGTCAGACTAAATGTAGGCTTTCAAGAAGACCTCAT GAACACTTCCTCCGCCCTCTATAGGTCCTACAAGACCGACTTGGAAACAGCGTTCCGGAAGGGTTACGGA ATTTTACCAGGCTTCAAGGGCGTGACTGTGACAGGGTTCAAGTCTGGAAGTGTGGTTGTGACATATGAAG TCAAGACTACACCACCATCACTTGAGTTAATACATAAAGCCAATGAACAAGTTGTACAGAGCCTCAATCA GACCTACAAAATGGACTACAACTCCTTTCAAGCAGTTACTATCAATGAAAGCAATTTCTTTGTCACACCA GAAATCATCTTTGAAGGGGACACAGTCAGTCTGGTGTGTGAAAAGGAAGTTTTGTCCTCCAATGTGTCTT GGCGCTATGAAGAACAGCAGTTGGAAATCCAGAACAGCAGCAGATTCTCGATTTACACCGCACTTTTCAA CAACATGACTTCGGTGTCCAAGCTCACCATCCACAACATCACTCCAGGTGATGCAGGTGAATATGTTTGC AAACTGATATTAGACATTTTTGAATATGAGTGCAAGAAGAAAATAGATGTTATGCCCATCCAAATTTTGG CAAATGAAGAAATGAAGGTGATGTGCGACAACAATCCTGTATCTTTGAACTGCTGCAGTCAGGGTAATGT TAATTGGAGCAAAGTAGAATGGAAGCAGGAAGGAAAAATAAATATTCCAGGAACCCCTGAGACAGACATA GATTCTAGCTGCAGCAGATACACCCTCAAGGCTGATGGAACCCAGTGCCCAAGCGGGTCGTCTGGAACAA CAGTCATCTACACTTGTGAGTTCATCAGTGCCTATGGAGCCAGAGGCAGTGCAAACATAAAAGTGACATT CATCTCTGTGGCCAATCTAACAATAACCCCGGACCCAATTTCTGTTTCTGAGGGACAAAACTTTTCTATA AAATGCATCAGTGATGTGAGTAACTATGATGAGGTTTATTGGAACACTTCTGCTGGAATTAAAATATACC AAAGATTTTATACCACGAGGAGGTATCTTGATGGAGCAGAATCAGTACTGACAGTCAAGACCTCGACCAG GGAGTGGAATGGAACCTATCACTGCATATTTAGATATAAGAATTCATACAGTATTGCAACCAAAGACGTC ATTGTTCACCCGCTGCCTCTAAAGCTGAACATCATGGTTGATCCTTTGGAAGCTACTGTTTCATGCAGTG GTTCCCATCACATCAAGTGCTGCATAGAGGAGGATGGAGACTACAAAGTTACTTTCCATATGGGTTCCTC ATCCCTTCCTGCTGCAAAAGAAGTTAACAAAAAACAAGTGTGCTACAAACACAATTTCAATGCAAGCTCA GTTTCCTGGTGTTCAAAAACTGTTGATGTGTGTTGTCACTTTACCAATGCTGCTAATAATTCAGTTTGGA GCCCATCTATGAAGCTGAATCTGGTTCCTGGGGAAAACATCACATGCCAGGATCCCGTAATAGGTGTCGG AGAGCCGGGGAAAGTCATCCAGAAGCTATGCCGGTTCTCAAACGTTCCCAGCAGCCCTGAGAGTCCCATT GGCGGGACCATCACTTACAAATGTGTAGGCTCCCAGTGGGAGGAGAAGAGAAATGACTGCATCTCTGCCC CAATAAACAGTCTGCTCCAGATGGCTAAGGCTTTGATCAAGAGCCCCTCTCAGGATGAGATGCTCCCTAC ATACCTGAAGGATCTTTCTATTAGCATAGACAAAGCGGAACATGAAATCAGCTCTTCTCCTGGGAGTCTG GGAGCCATTATTAACATCCTTGATCTGCTCTCAACAGTTCCAACCCAAGTAAATTCAGAAATGATGACGC ACGTGCTCTCTACGGTTAATGTCATCCTTGGCAAGCCCGTCTTGAACACCTGGAAGGTTTTACAACAGCA ATGGACCAATCAGAGTTCACAGCTACTACATTCAGTGGAAAGATTTTCCCAAGCATTACAGTCAGGAGAT AGCCCTCCTTTGTCCTTCTCCCAAACTAATGTGCAGATGAGCAGCACGGTAATCAAGTCCAGCCACCCAG AAACCTATCAACAGAGGTTTGTTTTCCCATACTTTGACCTCTGGGGCAATGTGGTCATTGACAAGAGCTA TCTAGAAAACTTGCAGTCGGATTCGTCTATTGTCACCATGGCTTTCCCAACTCTCCAAGCCATCCTTGCT CAGGATATCCAGGAAAATAACTTTGCAGAGAGCTTAGTGATGACAACCACTGTCAGCCACAATACGACTA TGCCATTCAGGATTTCAATGACTTTTAAGAACAATAGCCCTTCAGGCGGCGAAACGAAGTGTGTCTTCTG GAACTTCAGGCTTGCCAACAACACAGGGGGGTGGGACAGCAGTGGGTGCTATGTTGAAGAAGGTGATGGG GACAATGTCACCTGTATCTGTGACCACCTAACATCATTCTCCATCCTCATGTCCCCTGACTCCCCAGATC CTAGTTCTCTCCTGGGAATACTCCTGGATATTATTTCTTATGTTGGGGTGGGCTTTTCCATCTTGAGCTT GGCAGCCTGTCTAGTTGTGGAAGCTGTGGTGTGGAAATCGGTGACCAAGAATCGGACTTCTTATATGCGC CACACCTGCATAGTGAATATCGCTGCCTCCCTTCTGGTCGCCAACACCTGGTTCATTGTGGTCGCTGCCA TCCAGGACAATCGCTACATACTCTGCAAGACAGCCTGTGTGGCTGCCACCTTCTTCATCCACTTCTTCTA CCTCAGCGTCTTCTTCTGGATGCTGACACTGGGCCTCATGCTGTTCTATCGCCTGGTTTTCATTCTGCAT GAAACAAGCAGGTCCACTCAGAAAGCCATTGCCTTCTGTCTTGGCTATGGCTGCCCACTTGCCATCTCGG TCATCACGCTGGGAGCCACCCAGCCCCGGGAAGTCTATACGAGGAAGAATGTCTGTTGGCTCAACTGGGA GGACACCAAGGCCCTGCTGGCTTTCGCCATCCCAGCACTGATCATTGTGGTGGTGAACATAACCATCACT ATTGTGGTCATCACCAAGATCCTGAGGCCTTCCATTGGAGACAAGCCATGCAAGCAGGAGAAGAGCAGCC TGTTTCAGATCAGCAAGAGCATTGGGGTCCTCACACCACTCTTGGGCCTCACTTGGGGTTTTGGTCTCAC CACTGTGTTCCCAGGGACCAACCTTGTGTTCCATATCATATTTGCCATCCTCAATGTCTTCCAGGGATTA TTCATTTTACTCTTTGGATGCCTCTGGGATCTGAAGGTACAGGAAGCTTTGCTGAATAAGTTTTCATTGT CGAGATGGTCTTCACAGCACTCAAAGTCAACATCCCTGGGTTCATCCACACCTGTGTTTTCTATGAGTTC TCCAATATCAAGGAGATTTAACAATTTGTTTGGTAAAACAGGAACGTATAATGTTTCCACCCCAGAAGCA ACCAGCTCATCCCTGGAAAACTCATCCAGTGCTTCTTCGTTGCTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209170 protein sequence
Red=Cloning site Green=Tags(s) MKSPRRTTLCLMFIVIYSSKAALNWNYESTIHPLSLHEHEPAGEEALRQKRAVATKSPTAEEYTVNIEIS FENASFLDPIKAYLNSLSFPIHGNNTDQITDILSINVTTVCRPAGNEIWCSCETGYGWPRERCLHNLICQ ERDVFLPGHHCSCLKELPPNGPFCLLQEDVTLNMRVRLNVGFQEDLMNTSSALYRSYKTDLETAFRKGYG ILPGFKGVTVTGFKSGSVVVTYEVKTTPPSLELIHKANEQVVQSLNQTYKMDYNSFQAVTINESNFFVTP EIIFEGDTVSLVCEKEVLSSNVSWRYEEQQLEIQNSSRFSIYTALFNNMTSVSKLTIHNITPGDAGEYVC KLILDIFEYECKKKIDVMPIQILANEEMKVMCDNNPVSLNCCSQGNVNWSKVEWKQEGKINIPGTPETDI DSSCSRYTLKADGTQCPSGSSGTTVIYTCEFISAYGARGSANIKVTFISVANLTITPDPISVSEGQNFSI KCISDVSNYDEVYWNTSAGIKIYQRFYTTRRYLDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDV IVHPLPLKLNIMVDPLEATVSCSGSHHIKCCIEEDGDYKVTFHMGSSSLPAAKEVNKKQVCYKHNFNASS VSWCSKTVDVCCHFTNAANNSVWSPSMKLNLVPGENITCQDPVIGVGEPGKVIQKLCRFSNVPSSPESPI GGTITYKCVGSQWEEKRNDCISAPINSLLQMAKALIKSPSQDEMLPTYLKDLSISIDKAEHEISSSPGSL GAIINILDLLSTVPTQVNSEMMTHVLSTVNVILGKPVLNTWKVLQQQWTNQSSQLLHSVERFSQALQSGD SPPLSFSQTNVQMSSTVIKSSHPETYQQRFVFPYFDLWGNVVIDKSYLENLQSDSSIVTMAFPTLQAILA QDIQENNFAESLVMTTTVSHNTTMPFRISMTFKNNSPSGGETKCVFWNFRLANNTGGWDSSGCYVEEGDG DNVTCICDHLTSFSILMSPDSPDPSSLLGILLDIISYVGVGFSILSLAACLVVEAVVWKSVTKNRTSYMR HTCIVNIAASLLVANTWFIVVAAIQDNRYILCKTACVAATFFIHFFYLSVFFWMLTLGLMLFYRLVFILH ETSRSTQKAIAFCLGYGCPLAISVITLGATQPREVYTRKNVCWLNWEDTKALLAFAIPALIIVVVNITIT IVVITKILRPSIGDKPCKQEKSSLFQISKSIGVLTPLLGLTWGFGLTTVFPGTNLVFHIIFAILNVFQGL FILLFGCLWDLKVQEALLNKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPEA TSSSLENSSSASSLLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015234 |
| ORF Size | 4041 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015234.3, NP_056049.3 |
| RefSeq Size | 5762 bp |
| RefSeq ORF | 4041 bp |
| Locus ID | 221395 |
| UniProt ID | Q8IZF2 |
| Domains | GPS, 7tm_2, ig, IGc2, IG |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| MW | 149.5 kDa |
| Gene Summary | Receptor that plays a critical role in lung surfactant homeostasis. May play a role in controlling adipocyte function.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Orphan GPR116 mediates the insulin sensitizing effects of the hepatokine FNDC4 in adipose tissue
,null,
Nature Communications
,PubMed ID 34016966
[GPR116]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209170L1 | Lenti-ORF clone of GPR116 (Myc-DDK-tagged)-Human G protein-coupled receptor 116 (GPR116), transcript variant 1 |
CNY 15,300.00 |
|
| RC209170L2 | Lenti-ORF clone of GPR116 (mGFP-tagged)-Human G protein-coupled receptor 116 (GPR116), transcript variant 1 |
CNY 16,224.00 |
|
| RC209170L3 | Lenti-ORF clone of GPR116 (Myc-DDK-tagged)-Human G protein-coupled receptor 116 (GPR116), transcript variant 1 |
CNY 16,224.00 |
|
| RC209170L4 | Lenti-ORF clone of GPR116 (mGFP-tagged)-Human G protein-coupled receptor 116 (GPR116), transcript variant 1 |
CNY 15,300.00 |
|
| RG209170 | GPR116 (tGFP-tagged) - Human G protein-coupled receptor 116 (GPR116), transcript variant 1 |
CNY 15,424.00 |
|
| SC100690 | GPR116 (untagged)-Human G protein-coupled receptor 116 (GPR116), transcript variant 1 |
CNY 13,824.00 |
