United States
Germany
Japan
United Kingdom
China
HHAT (NM_018194) Human Tagged ORF Clone
CAT＃: RC208447
- TrueORF®
HHAT (Myc-DDK-tagged)-Human hedgehog acyltransferase (HHAT), transcript variant 1
ORF Plasmid: tGFP
"NM_018194" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MART2; SKI1; Skn |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208447 representing NM_018194
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCCCGATGGGAACTGGCACTTTACCTACTTGCCTCACTAGGCTTCCACTTCTATTCCTTCTATG AAGTTTACAAAGTCTCCAGAGAACACGAAGAGGAGCTGGACCAGGAATTTGAGCTGGAGACTGACACTTT ATTTGGAGGATTAAAGAAGGATGCGACCGACTTTGAGTGGAGCTTCTGGATGGAATGGGGGAAGCAGTGG CTGGTGTGGCTTCTCCTTGGCCACATGGTAGTGTCTCAAATGGCCACACTGCTGGCAAGAAAGCACAGAC CCTGGATTCTCATGCTCTATGGGATGTGGGCCTGCTGGTGTGTGCTGGGGACCCCTGGTGTGGCTATGGT TTTGCTCCATACCACCATCTCTTTCTGCGTGGCCCAGTTCCGGTCTCAGCTCCTGACGTGGCTCTGTTCT CTCCTCCTCCTCTCCACACTGAGGCTGCAGGGTGTGGAAGAAGTTAAGAGAAGGTGGTACAAGACAGAAA ACGAGTACTACCTGCTGCAGTTCACGCTGACCGTTCGCTGCCTGTACTACACCAGCTTCAGCCTGGAGCT CTGCTGGCAGCAGCTGCCTGCTGCATCGACCTCCTACTCCTTTCCCTGGATGCTGGCCTATGTCTTTTAT TATCCAGTCTTACACAATGGGCCCATCCTCAGCTTCTCGGAGTTCATCAAACAGATGCAGCAGCAGGAAC ATGACTCCCTGAAGGCCAGCCTGTGTGTCCTGGCCCTGGGCTGGGCCGCCTTCTTTGCTGGTGGTGGCTG GCCGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208447 representing NM_018194
Red=Cloning site Green=Tags(s) MLPRWELALYLLASLGFHFYSFYEVYKVSREHEEELDQEFELETDTLFGGLKKDATDFEWSFWMEWGKQW LVWLLLGHMVVSQMATLLARKHRPWILMLYGMWACWCVLGTPGVAMVLLHTTISFCVAQFRSQLLTWLCS LLLLSTLRLQGVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCWQQLPAASTSYSFPWMLAYVFY YPVLHNGPILSFSEFIKQMQQQEHDSLKASLCVLALGWAAFFAGGGWPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018194 |
| ORF Size | 777 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 3598 bp |
| RefSeq ORF | 1482 bp |
| Locus ID | 55733 |
| UniProt ID | Q5VTY9 |
| Domains | MBOAT |
| Protein Families | Transmembrane |
| MW | 30.51 kDa |
| Gene Summary | 'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725).[supplied by OMIM, Jul 2002] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208447L1 | Lenti ORF clone of Human hedgehog acyltransferase (HHAT), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC208447L2 | Lenti ORF clone of Human hedgehog acyltransferase (HHAT), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC208447L3 | Lenti ORF clone of Human hedgehog acyltransferase (HHAT), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC208447L4 | Lenti ORF clone of Human hedgehog acyltransferase (HHAT), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG208447 | HHAT (tGFP-tagged) - Human hedgehog acyltransferase (HHAT), transcript variant 1 |
CNY 4,000.00 |
|
| SC113635 | HHAT (untagged)-Human hedgehog acyltransferase (HHAT), transcript variant 1 |
CNY 3,656.00 |
