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DNAAF11 (NM_012472) Human Tagged ORF Clone
CAT＃: RC208256
LRRC6 (Myc-DDK-tagged)-Human leucine rich repeat containing 6 (LRRC6)
ORF Plasmid: tGFP
"NM_012472" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CILD19; LRRC6; LRTP; tilB; TSLRP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208256 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGGATCACAGAAGATCTTATTAGACGGAATGCTGAACACAACGACTGTGTCATTTTTTCCCTGG AGGAACTCTCGTTGCATCAGCAAGAAATAGAAAGACTAGAACACATTGATAAATGGTGCCGGGATTTAAA AATTCTCTATCTTCAAAATAATCTTATTGGGAAAATTGAAAATGTTAGCAAACTCAAGAAACTTGAATAT TTGAATTTAGCTTTAAACAACATTGAAAAAATAGAAAACTTGGAAGGATGTGAAGAGCTGGCAAAACTTG ACCTGACTGTGAATTTCATTGGAGAGCTGAGCAGCATTAAAAACTTGCAGCACAATATCCATCTGAAGGA GCTCTTTCTCATGGGGAACCCATGTGCTTCCTTTGACCACTATAGGGAGTTCGTGGTAGCAACTCTTCCA CAATTAAAGTGGTTGGATGGTAAAGAAATAGAGCCTTCAGAAAGGATTAAGGCATTGCAGGACTATTCAG TAATTGAACCACAAATCAGAGAGCAGGAAAAAGATCACTGTCTTAAACGAGCCAAACTCAAGGAAGAGGC TCAGAGGAAACACCAAGAAGAGGATAAAAATGAAGACAAGAGAAGTAACGCAGGCTTTGATGGACGTTGG TACACAGACATCAATGCTACTCTTTCCTCTTTAGAGAGCAAAGACCACCTACAGGCACCAGACACAGAGG AACACAACACAAAGAAATTAGACAACAGTGAAGATGACTTGGAATTCTGGAATAAGCCCTGTTTGTTTAC TCCTGAATCAAGATTGGAAACTCTTAGACACATGGAAAAACAACGGAAGAAACAGGAAAAATTAAGTGAA AAAAAGAAGAAAGTGAAACCACCCAGGACTTTGATCACTGAAGATGGGAAAGCCCTAAATGTGAATGAGC CCAAAATTGACTTCTCTTTGAAAGATAACGAAAAGCAGATCATCCTGGACCTTGCTGTCTATAGGTATAT GGATACCTCTTTAATCGATGTTGATGTGCAACCAACTTACGTGCGAGTAATGATCAAAGGAAAGCCATTT CAGCTTGTCCTTCCTGCAGAAGTGAAACCCGATAGTAGTTCTGCTAAAAGATCTCAGACAACGGGTCATT TGGTCATCTGCATGCCCAAGGTAGGAGAAGTAATCACAGGTGGTCAGCGAGCATTCAAATCTATGAAAAC TACCTCGGACAGGAGCAGAGAACAAACAAATACAAGAAGCAAGCACATGGAGAAACTAGAAGTAGACCCT AGCAAGCACTCATTCCCTGATGTGACTAACATAGTTCAAGAGAAAAAACACACACCCAGAAGACGACCTG AACCCAAAATTATACCAAGTGAGGAAGACCCAACCTTTGAAGACAACCCTGAAGTGCCTCCGCTGATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208256 protein sequence
Red=Cloning site Green=Tags(s) MGWITEDLIRRNAEHNDCVIFSLEELSLHQQEIERLEHIDKWCRDLKILYLQNNLIGKIENVSKLKKLEY LNLALNNIEKIENLEGCEELAKLDLTVNFIGELSSIKNLQHNIHLKELFLMGNPCASFDHYREFVVATLP QLKWLDGKEIEPSERIKALQDYSVIEPQIREQEKDHCLKRAKLKEEAQRKHQEEDKNEDKRSNAGFDGRW YTDINATLSSLESKDHLQAPDTEEHNTKKLDNSEDDLEFWNKPCLFTPESRLETLRHMEKQRKKQEKLSE KKKKVKPPRTLITEDGKALNVNEPKIDFSLKDNEKQIILDLAVYRYMDTSLIDVDVQPTYVRVMIKGKPF QLVLPAEVKPDSSSAKRSQTTGHLVICMPKVGEVITGGQRAFKSMKTTSDRSREQTNTRSKHMEKLEVDP SKHSFPDVTNIVQEKKHTPRRRPEPKIIPSEEDPTFEDNPEVPPLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012472 |
| ORF Size | 1398 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012472.6 |
| RefSeq Size | 1888 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 23639 |
| UniProt ID | Q86X45 |
| Domains | LRR, LRRcap, LRR_SD22 |
| MW | 54.3 kDa |
| Gene Summary | The protein encoded by this gene contains several leucine-rich repeat domains and appears to be involved in the motility of cilia. Defects in this gene are a cause of primary ciliary dyskinesia-19 (CILD19). Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4, 11 and 22. [provided by RefSeq, Apr 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208256L3 | Lenti ORF clone of Human leucine rich repeat containing 6 (LRRC6), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208256L4 | Lenti ORF clone of Human leucine rich repeat containing 6 (LRRC6), mGFP tagged |
CNY 5,890.00 |
|
| RG208256 | LRRC6 (tGFP-tagged) - Human leucine rich repeat containing 6 (LRRC6) |
CNY 4,370.00 |
|
| SC322640 | LRRC6 (untagged)-Human leucine rich repeat containing 6 (LRRC6) |
CNY 3,656.00 |
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