GPBAR1 (NM_001077191) Human Tagged ORF Clone
CAT#: RC207164
GPBAR1 (Myc-DDK-tagged)-Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 1
ORF Plasmid: tGFP
"NM_001077191" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Cited in 4 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BG37; GPCR19; GPR131; M-BAR; TGR5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207164 representing NM_001077191.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACGCCCAACAGCACTGGCGAGGTGCCCAGCCCCATTCCCAAGGGGGCTTTGGGGCTCTCCCTGGCC CTGGCAAGCCTCATCATCACCGCGAACCTGCTCCTAGCCCTGGGCATCGCCTGGGACCGCCGCCTGCGC AGCCCACCTGCTGGCTGCTTCTTCCTGAGCCTACTGCTGGCTGGGCTGCTCACGGGTCTGGCATTGCCC ACATTGCCAGGGCTGTGGAACCAGAGTCGCCGGGGTTACTGGTCCTGCCTCCTCGTCTACTTGGCTCCC AACTTCTCCTTCCTCTCCCTGCTTGCCAACCTCTTGCTGGTGCACGGGGAGCGCTACATGGCAGTCCTG AGGCCACTCCAGCCCCCTGGGAGCATTCGGCTGGCCCTGCTCCTCACCTGGGCTGGTCCCCTGCTCTTT GCCAGTCTGCCCGCTCTTGGGTGGAACCACTGGACCCCTGGTGCCAACTGCAGCTCCCAGGCTATCTTC CCAGCCCCCTACCTGTACCTCGAAGTCTATGGGCTCCTGCTGCCCGCCGTGGGTGCTGCTGCCTTCCTC TCTGTCCGCGTGCTGGCCACTGCCCACCGCCAGCTGCAGGACATCTGCCGGCTGGAGCGGGCAGTGTGC CGCGATGAGCCCTCCGCCCTGGCCCGGGCCCTTACCTGGAGGCAGGCAAGGGCACAGGCTGGAGCCATG CTGCTCTTCGGGCTGTGCTGGGGGCCCTACGTGGCCACACTGCTCCTCTCAGTCCTGGCCTATGAGCAG CGCCCGCCACTGGGGCCTGGGACACTGTTGTCCCTCCTCTCCCTAGGAAGTGCCAGTGCAGCGGCAGTG CCCGTAGCCATGGGGCTGGGCGATCAGCGCTACACAGCCCCCTGGAGGGCAGCCGCCCAAAGGTGCCTG CAGGGGCTGTGGGGAAGAGCCTCCCGGGACAGTCCCGGCCCCAGCATTGCCTACCACCCAAGCAGCCAA AGCAGTGTCGACCTGGACTTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC207164
Blue=ORF Red=Cloning site Green=Tag(s) MTPNSTGEVPSPIPKGALGLSLALASLIITANLLLALGIAWDRRLRSPPAGCFFLSLLLAGLLTGLALP TLPGLWNQSRRGYWSCLLVYLAPNFSFLSLLANLLLVHGERYMAVLRPLQPPGSIRLALLLTWAGPLLF ASLPALGWNHWTPGANCSSQAIFPAPYLYLEVYGLLLPAVGAAAFLSVRVLATAHRQLQDICRLERAVC RDEPSALARALTWRQARAQAGAMLLFGLCWGPYVATLLLSVLAYEQRPPLGPGTLLSLLSLGSASAAAV PVAMGLGDQRYTAPWRAAAQRCLQGLWGRASRDSPGPSIAYHPSSQSSVDLDLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001077191 |
ORF Size | 990 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 2023 bp |
RefSeq ORF | 993 bp |
Locus ID | 151306 |
UniProt ID | Q8TDU6 |
Protein Families | Druggable Genome |
MW | 35.2 kDa |
Gene Summary | This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008] |
Citations (4)
The use of this cDNA Clones has been cited in the following citations: |
---|
Bile Acids Gate Dopamine Transporter Mediated Currents
,null,
Frontiers in Chemistry
,PubMed ID 34957043
[GPBAR1]
|
Ursolic acid activates the TGR5 receptor to enhance GLP-1 secretion in type 1-like diabetic rats
,Lo, SH;Li, Y;Cheng, KC;Niu, CS;Cheng, JT;Niu, HS;,
Naunyn Schmiedebergs Arch. Pharmacol.
,PubMed ID 28756460
[GPBAR1]
|
Investigation of triamterene as an inhibitor of the TGR5 receptor: identification in cells and animals
,Li, Y;Cheng, KC;Niu, CS;Lo, SH;Cheng, JT;Niu, HS;,
Drug Des Devel Ther
,PubMed ID 28435224
[GPBAR1]
|
TGR5 activation suppressed S1P/S1P2 signaling and resisted high glucose-induced fibrosis in glomerular mesangial cells
,Yang, Z;Xiong, F;Wang, Y;Gong, W;Huang, J;Chen, C;Liu, P;Huang, H;,
Pharmacol. Res.
,PubMed ID 27317945
[GPBAR1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207164L1 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC207164L2 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC207164L3 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC207164L4 | Lenti ORF clone of Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
RG207164 | GPBAR1 (tGFP-tagged) - Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 1 |
CNY 4,000.00 |
|
SC323958 | GPBAR1 (untagged)-Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 1 |
CNY 2,400.00 |