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BLK (NM_001715) Human Tagged ORF Clone
CAT＃: RC207123
- TrueORF®
BLK (Myc-DDK-tagged)-Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_001715" in other vectors (6)
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CNY 3,760.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MODY11 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207123 ORF sequence, codon optimized.
Due to the complexity of NM_001715, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCTGGTATCCTCTAAGAAACCCGACAAGGAAAAACCCATTAAGGAAAAGGACAAGGGACAGTGGA GCCCCCTTAAGGTGTCAGCGCAGGATAAAGACGCACCTCCCCTGCCCCCTCTGGTTGTGTTTAACCACTT GACCCCTCCTCCACCCGACGAGCACCTGGATGAGGACAAACACTTCGTGGTCGCCCTCTACGATTACACA GCTATGAATGATCGCGATCTCCAGATGCTGAAGGGGGAGAAACTTCAAGTCCTCAAAGGCACTGGCGACT GGTGGCTGGCCCGATCCCTGGTAACGGGCCGCGAGGGCTACGTTCCCAGCAATTTTGTCGCTCGCGTGGA GTCTTTGGAGATGGAAAGGTGGTTCTTTAGATCTCAAGGCAGGAAAGAAGCTGAAAGACAGCTCCTGGCG CCAATTAATAAAGCAGGCAGTTTCCTGATCAGAGAGAGTGAAACGAATAAGGGAGCGTTTTCTCTCTCAG TCAAAGACGTGACTACCCAGGGAGAACTGATTAAGCACTACAAAATCCGCTGCCTTGATGAAGGTGGCTA CTATATTTCCCCCAGAATTACTTTTCCTTCACTGCAGGCCCTGGTCCAGCACTACAGCAAGAAGGGAGAT GGTTTGTGTCAGCGCCTGACACTCCCATGTGTTCGGCCAGCCCCCCAGAATCCATGGGCTCAAGACGAAT GGGAAATCCCCCGGCAAAGTCTCAGACTTGTGAGGAAGCTGGGGAGTGGACAATTTGGGGAGGTCTGGAT GGGCTACTACAAGAATAACATGAAGGTGGCAATTAAAACCCTGAAAGAAGGGACTATGAGTCCGGAGGCA TTTCTGGGCGAGGCAAATGTGATGAAGGCCTTGCAGCACGAGCGCCTGGTAAGGTTGTATGCCGTGGTGA CTAAGGAGCCCATCTACATCGTAACCGAATACATGGCAAGGGGGTGCCTGCTTGACTTCTTGAAAACAGA CGAGGGCTCCAGACTGTCCCTGCCCCGGCTGATCGATATGTCAGCACAGATCGCCGAGGGCATGGCTTAC ATCGAAAGGATGAACTCCATACACCGAGATCTTCGCGCAGCCAACATCTTGGTGTCTGAAGCTCTGTGTT GCAAGATCGCCGACTTCGGGCTCGCTCGGATCATCGATAGCGAATATACTGCCCAAGAGGGTGCCAAGTT CCCGATCAAATGGACCGCCCCCGAGGCCATCCACTTCGGCGTCTTCACAATTAAGGCTGACGTGTGGTCC TTTGGGGTCCTGCTTATGGAGGTGGTTACATACGGTCGGGTTCCCTACCCTGGTATGTCCAACCCCGAGG TGATTCGAAACCTTGAGAGGGGCTATCGGATGCCCCGGCCCGACACCTGCCCACCCGAGCTGTATAGAGG CGTTATTGCTGAATGCTGGCGGTCCCGGCCTGAGGAACGCCCAACATTCGAGTTTTTGCAATCAGTGCTG GAAGATTTCTATACCGCCACTGAACGGCAGTACGAACTGCAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207123 representing NM_001715
Red=Cloning site Green=Tags(s) MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYT AMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLA PINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGD GLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEA FLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAY IERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWS FGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVL EDFYTATERQYELQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001715 |
| ORF Size | 1515 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001715.1, NM_001715.2, NP_001706.2 |
| RefSeq Size | 2215 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 640 |
| UniProt ID | P51451 |
| Domains | pkinase, SH2, TyrKc, SH3, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 57.7 kDa |
| Gene Summary | This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207123L3 | Lenti-ORF clone of BLK (Myc-DDK-tagged)-Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized |
CNY 5,890.00 |
|
| RC207123L4 | Lenti-ORF clone of BLK (mGFP-tagged)-Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized |
CNY 5,890.00 |
|
| RG207123 | BLK (tGFP-tagged) - Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized |
CNY 4,370.00 |
|
| SC119056 | BLK (untagged)-Human B lymphoid tyrosine kinase (BLK) |
CNY 2,950.00 |
|
| SC323504 | BLK (untagged)-Kinase deficient mutant (K269M) of Human B lymphoid tyrosine kinase (BLK) |
CNY 3,768.00 |
|
| SC323517 | BLK (untagged)-Kinase deficient mutant (K269M) of Human B lymphoid tyrosine kinase (BLK) |
CNY 3,768.00 |
