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DENND2B (NM_213618) Human Tagged ORF Clone
CAT＃: RC206037
ST5 (Myc-DDK-tagged)-Human suppression of tumorigenicity 5 (ST5), transcript variant 3
ORF Plasmid: tGFP
"NM_213618" in other vectors (4)
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CNY 7,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HTS1; p126; ST5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206037 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGACTGCCAACAAGAATTCCAGCATCACCCACGGAGCTGGTGGCACTAAAGCCCCTCGGGGGA CTCTGAGCAGGTCTCAGTCAGTCTCTCCACCTCCAGTTCTCTCCCCACCAAGGAGTCCCATCTACCCGCT CAGTGATAGTGAAACCTCAGCCTGCAGGTACCCCAGCCACTCCAGCTCCCGGGTGCTCCTCAAGGACCGG CACCCCCCAGCTCCTTCACCCCAGAATCCTCAAGATCCCTCCCCAGATACTTCCCCACCCACCTGTCCCT TCAAGACCGCCAGCTTCGGTTATTTGGACAGAAGCCCTTCGGCGTGCAAGAGAGACGCCCAAAAGGAAAG TGTCCAAGGCGCAGCCCAGGATGTAGCAGGGGTCGCTGCCTGCCTCCCCCTTGCCCAGAGCACGCCATTC CCGGGGCCAGCAGCTGGCCCCCGGGGCGTCTTGCTGACCCGTACCGGTACCCGCGCCCACAGCCTGGGCA TCCGGGAGAAGATATCAGCATGGGAAGGTCGCCGAGAGGCGTCGCCCAGGATGAGCATGTGTGGAGAGAA GCGGGAGGGCTCTGGGAGCGAGTGGGCGGCCAGTGAGGGCTGCCCCAGCCTGGGCTGTCCCAGCGTGGTG CCGTCCCCCTGCAGCTCTGAAAAGACCTTTGATTTCAAGGGCCTCCGGAGGATGAGCAGGACCTTCTCCG AGTGTTCCTACCCAGAGACTGAGGAGGAGGGAGAGGCGCTCCCTGTCCGGGACTCTTTCTACCGGCTGGA GAAACGGCTGGGCCGGAGTGAGCCCAGCGCCTTCCTCAGGGGGCATGGCAGCAGGAAGGAGAGCTCAGCA GTGCTGAGCCGGATCCAGAAAATTGAACAGGTCCTGAAGGAGCAGCCGGGCCGGGGGCTCCCCCAGCTCC CCAGCAGCTGCTACAGCGTGGACCGGGGGAAAAGGAACACTGGAACCTTGGGCTCCTTGGAGGAGCCGGC AGGGGGCGCGAGTGTGAGCGCTGGCAGCCGGGCAGTCGGAGTGGCTGGTGTTGCGGGGGAGGCGGGCCCA CCCCCAGAGAGGGAAGGCAGTGGTTCCACTAAGCCCGGGACCCCTGGAAATAGCCCTAGCTCCCAGCGGC TGCCATCGAAGAGTTCCCTCGATCCCGCTGTGAACCCTGTCCCCAAACCCAAGCGCACCTTTGAATACGA GGCTGACAAGAACCCCAAGAGTAAGCCCAGTAATGGTCTACCTCCTTCACCCACACCTGCTGCTCCACCT CCCTTGCCCTCCACCCCAGCCCCGCCAGTCACCCGGAGACCCAAGAAGGACATGCGTGGTCACCGCAAGT CCCAGAGCAGAAAATCCTTTGAGTTTGAGGATGCATCCAGTCTCCAGTCCCTGTACCCCTCTTCTCCCAC TGAGAATGGTACTGAGAACCAACCCAAGTTTGGATCCAAAAGCACTTTAGAAGAAAATGCCTATGAAGAT ATTGTGGGAGATCTGCCCAAGGAGAATCCATATGAGGATGTGGACTTAAAGAGCCGAAGAGCAGGACGAA AATCCCAGCAACTGTCTGAGAACTCCTTGGACTCTTTGCACAGGATGTGGAGTCCTCAGGACAGGAAGTA CAACAGCCCGCCCACACAGCTTTCCCTGAAACCCAACAGCCAGTCCCTGCGCAGTGGGAACTGGTCAGAA AGGAAGAGCCACCGGCTGCCACGATTACCCAAGAGGCACAGCCATGACGACATGCTGCTGCTGGCTCAGC TGAGTCTGCCGTCCTCACCCTCCAGCCTCAATGAAGACAGCCTCAGCACCACCAGCGAGCTGCTGTCCAG CCGCCGGGCCCGCCGCATTCCCAAGCTTGTCCAAAGAACTAACTCCATCTACAATGCCAAGAGAGGAAAG AAGAGATTAAAAAAGTTGTCTATGTCCAGCATTGAAACAGCATCACTGAGAGATGAAAACAGTGAGAGCG AGAGCGACTCTGATGACAGGTTCAAAGCCCACACACAGCGCCTGGTCCACATCCAGTCGATGCTGAAGCG CGCCCCCAGCTATCGCACGCTGGAGCTGGAGCTGCTGGAGTGGCAGGAGCGGGAGCTTTTTGAGTACTTT GTGGTGGTGTCCCTCAAGAAGAAGCCATCGCGAAACACCTACCTCCCCGAAGTCTCCTACCAGTTTCCCA AGCTGGACCGACCCACCAAGCAGATGCGAGAGGCAGAGGAAAGGCTCAAAGCCATTCCCCAGTTTTGCTT CCCTGATGCCAAGGACTGGCTTCCTGTGTCAGAGTATAGCAGTGAGACCTTTTCTTTCATGCTGACTGGG GAAGATGGCGGCAGACGCTTTGGCTACTGCAGGCGCTTACTGCCAAGTGGGAAAGGGCCCCGGTTGCCAG AGGTGTACTGTGTCATCAGCCGCCTTGGCTGCTTCGGCTTGTTTTCCAAGGTCCTAGATGAGGTGGAGCG CCGGCGTGGGATCTCCGCTGCATTGGTCTATCCTTTCATGAGAAGTCTCATGGAGTCGCCCTTCCCAGCC CCAGGGAAGACCATCAAAGTGAAGACATTCCTGCCAGGTGCTGGCAATGAGGTGTTAGAGCTGCGGCGGC CCATGGACTCAAGGCTGGAGCACGTGGACTTTGAGTGCCTTTTTACCTGCCTCAGTGTGCGCCAGCTCAT CCGAATCTTTGCCTCACTGCTGCTGGAGCGCCGGGTCATTTTTGTGGCAGATAAGCTCAGTACCCTCTCC AGCTGCTCCCACGCGGTGGTGGCCTTGCTCTACCCCTTCTCCTGGCAGCACACCTTCATTCCTGTCCTCC CGGCCTCCATGATTGACATCGTCTGCTGTCCCACCCCCTTCCTGGTTGGCCTGCTCTCCAGCTCCCTCCC CAAACTGAAGGAGCTGCCTGTGGAGGAGGCGCTGATGGTGAATCTGGGATCTGACCGATTCATCCGACAG ATGGACGACGAAGACACGTTGTTACCTAGGAAGTTACAGGCAGCTCTGGAGCAGGCTCTGGAGAGGAAGA ATGAGCTGATCTCCCAGGACTCTGACAGCGACTCCGACGATGAATGTAATACCCTCAATGGGCTGGTGTC GGAGGTGTTTATCCGGTTCTTTGTGGAGACCGTTGGGCACTACTCCCTCTTTCTGACACAGAGTGAGAAG GGAGAGAGGGCCTTTCAGCGAGAGGCCTTCCGCAAATCTGTGGCCTCCAAAAGCATCCGCCGCTTTCTTG AGGTTTTTATGGAGTCTCAGATGTTTGCTGGCTTCATCCAAGACAGGGAGCTAAGAAAGTGTCGGGCAAA GGGCCTTTTTGAGCAGCGAGTGGAGCAGTACTTAGAAGAACTCCCAGACACTGAGCAGAGTGGAATGAAT AAGTTTCTCCGAGGTTTGGGCAACAAAATGAAGTTTCTCCACAAGAAGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206037 protein sequence
Red=Cloning site Green=Tags(s) MTMTANKNSSITHGAGGTKAPRGTLSRSQSVSPPPVLSPPRSPIYPLSDSETSACRYPSHSSSRVLLKDR HPPAPSPQNPQDPSPDTSPPTCPFKTASFGYLDRSPSACKRDAQKESVQGAAQDVAGVAACLPLAQSTPF PGPAAGPRGVLLTRTGTRAHSLGIREKISAWEGRREASPRMSMCGEKREGSGSEWAASEGCPSLGCPSVV PSPCSSEKTFDFKGLRRMSRTFSECSYPETEEEGEALPVRDSFYRLEKRLGRSEPSAFLRGHGSRKESSA VLSRIQKIEQVLKEQPGRGLPQLPSSCYSVDRGKRNTGTLGSLEEPAGGASVSAGSRAVGVAGVAGEAGP PPEREGSGSTKPGTPGNSPSSQRLPSKSSLDPAVNPVPKPKRTFEYEADKNPKSKPSNGLPPSPTPAAPP PLPSTPAPPVTRRPKKDMRGHRKSQSRKSFEFEDASSLQSLYPSSPTENGTENQPKFGSKSTLEENAYED IVGDLPKENPYEDVDLKSRRAGRKSQQLSENSLDSLHRMWSPQDRKYNSPPTQLSLKPNSQSLRSGNWSE RKSHRLPRLPKRHSHDDMLLLAQLSLPSSPSSLNEDSLSTTSELLSSRRARRIPKLVQRTNSIYNAKRGK KRLKKLSMSSIETASLRDENSESESDSDDRFKAHTQRLVHIQSMLKRAPSYRTLELELLEWQERELFEYF VVVSLKKKPSRNTYLPEVSYQFPKLDRPTKQMREAEERLKAIPQFCFPDAKDWLPVSEYSSETFSFMLTG EDGGRRFGYCRRLLPSGKGPRLPEVYCVISRLGCFGLFSKVLDEVERRRGISAALVYPFMRSLMESPFPA PGKTIKVKTFLPGAGNEVLELRRPMDSRLEHVDFECLFTCLSVRQLIRIFASLLLERRVIFVADKLSTLS SCSHAVVALLYPFSWQHTFIPVLPASMIDIVCCPTPFLVGLLSSSLPKLKELPVEEALMVNLGSDRFIRQ MDDEDTLLPRKLQAALEQALERKNELISQDSDSDSDDECNTLNGLVSEVFIRFFVETVGHYSLFLTQSEK GERAFQREAFRKSVASKSIRRFLEVFMESQMFAGFIQDRELRKCRAKGLFEQRVEQYLEELPDTEQSGMN KFLRGLGNKMKFLHKKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_213618 |
| ORF Size | 3411 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_213618.1, NP_998783.1 |
| RefSeq Size | 4371 bp |
| RefSeq ORF | 3414 bp |
| Locus ID | 6764 |
| UniProt ID | P78524 |
| MW | 126.4 kDa |
| Gene Summary | This gene was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein encoded by this gene contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells. Three alternatively spliced transcript variants of this gene encoding distinct isoforms are identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206037L3 | Lenti ORF clone of Human suppression of tumorigenicity 5 (ST5), transcript variant 3, Myc-DDK-tagged |
CNY 11,590.00 |
|
| RC206037L4 | Lenti ORF clone of Human suppression of tumorigenicity 5 (ST5), transcript variant 3, mGFP tagged |
CNY 11,590.00 |
|
| RG206037 | ST5 (tGFP-tagged) - Human suppression of tumorigenicity 5 (ST5), transcript variant 3 |
CNY 9,520.00 |
|
| SC308681 | ST5 (untagged)-Human suppression of tumorigenicity 5 (ST5), transcript variant 3 |
CNY 10,450.00 |
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