Epithelial Stromal Interaction 1 (EPSTI1) (NM_001002264) Human Tagged ORF Clone
CAT#: RC205699
EPSTI1 (Myc-DDK-tagged)-Human epithelial stromal interaction 1 (breast) (EPSTI1), transcript variant 1
ORF Plasmid: tGFP
"NM_001002264" in other vectors (4)
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CNY 5,488.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BRESI1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205699 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACCCGCAATAGAGTGGTGAACTCCGGGCTCGGCGCCTCCCCTGCCTCCCGCCCGACCCGGGATC CCCAGGACCCTTCTGGGCGGCAAGGGGAGCTGAGCCCCGTGGAAGACCAGAGAGAGGGTTTGGAGGCAGC CCCTAAGGGCCCTTCGCGGGAGAGCGTCGTGCACGCGGGCCAGAGGCGCACAAGTGCATACACCTTGATA GCACCAAATATAAACCGGAGAAATGAGATACAAAGAATTGCGGAGCAGGAGCTGGCCAACCTGGAGAAGT GGAAGGAGCAGAACAGAGCTAAACCGGTTCACCTGGTGCCCAGACGGCTAGGTGGAAGCCAGTCAGAAAC TGAAGTCAGACAGAAACAACAACTCCAGCTGATGCAATCTAAATACAAGCAAAAGCTAAAAAGAGAAGAA TCTGTAAGAATCAAGAAGGAAGCTGAAGAAGCTGAACTCCAAAAAATGAAGGCAATTCAGAGAGAGAAGA GCAATAAACTGGAGGAGAAAAAAAGACTTCAAGAAAACCTTAGAAGAGAAGCATTTAGAGAGCATCAGCA ATACAAAACCGCTGAGTTCTTGAGCAAACTGAACACAGAATCGCCAGACAGAAGTGCCTGTCAAAGTGCT GTTTGTGGCCCACAATCCTCAACATGGAAACTTCCTATCCTGCCTAGGGATCACAGCTGGGCCAGAAGCT GGGCTTACAGAGATTCTCTAAAGGCAGAAGAAAACAGAAAATTGCAAAAGATGAAGGATGAACAACATCA AAAGAGTGAATTACTGGAACTGAAACGGCAGCAGCAAGAGCAAGAAAGAGCCAAAATCCACCAGACTGAA CACAGGAGGGTAAATAATGCTTTTCTGGACCGACTCCAAGGCAAAAGTCAACCAGGTGGCCTCGAGCAAT CTGGAGGCTGTTGGAATATGAATAGCGGTAACAGCTGGGGTTCTCTATTAGTTTTTTCGAGGCACCTAAG GGTATATGAGAAAATATTGACTCCTATCTGGCCTTCATCAACTGACCTCGAAAAGCCTCATGAGATGCTT TTTCTTAATGTGATTTTGTTCAGCCTCACTGTTTTTACCTTAATTTCAACTGCCCACACACTTGACCGTG CAGTCAGGAGTGACTGGCTTCTCCTTGTCCTCATTTATGCATGTTTGGAGGAGCTGATTCCTGAACTCAT ATTTAATCTCTACTGCCAGGGAAATGCTACATTATTTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205699 protein sequence
Red=Cloning site Green=Tags(s) MNTRNRVVNSGLGASPASRPTRDPQDPSGRQGELSPVEDQREGLEAAPKGPSRESVVHAGQRRTSAYTLI APNINRRNEIQRIAEQELANLEKWKEQNRAKPVHLVPRRLGGSQSETEVRQKQQLQLMQSKYKQKLKREE SVRIKKEAEEAELQKMKAIQREKSNKLEEKKRLQENLRREAFREHQQYKTAEFLSKLNTESPDRSACQSA VCGPQSSTWKLPILPRDHSWARSWAYRDSLKAEENRKLQKMKDEQHQKSELLELKRQQQEQERAKIHQTE HRRVNNAFLDRLQGKSQPGGLEQSGGCWNMNSGNSWGSLLVFSRHLRVYEKILTPIWPSSTDLEKPHEML FLNVILFSLTVFTLISTAHTLDRAVRSDWLLLVLIYACLEELIPELIFNLYCQGNATLFF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001002264 |
ORF Size | 1230 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001002264.4 |
RefSeq Size | 3201 bp |
RefSeq ORF | 1233 bp |
Locus ID | 94240 |
UniProt ID | Q96J88 |
Protein Families | Transmembrane |
MW | 47.5 kDa |
Gene Summary | The protein encoded by this gene has been shown to promote tumor invasion and metastasis in some invasive cancer cells when overexpressed. Expression of this gene has been shown to be upregulated by direct binding of the Kruppel like factor 8 protein to promoter sequences. The translated protein interacts with the amino terminal region of the valosin containing protein gene product, resulting in the nuclear translocation of the nuclear factor kappa B subunit 1 gene product, and activation of target genes. Overexpression of this gene has been observed in some breast cancers and in some individuals with systemic lupus erythematosus (SLE). [provided by RefSeq, Sep 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205699L3 | Lenti ORF clone of Human epithelial stromal interaction 1 (breast) (EPSTI1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205699L4 | Lenti ORF clone of Human epithelial stromal interaction 1 (breast) (EPSTI1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG205699 | EPSTI1 (tGFP-tagged) - Human epithelial stromal interaction 1 (breast) (EPSTI1), transcript variant 1 |
CNY 7,088.00 |
|
SC300428 | EPSTI1 (untagged)-Human epithelial stromal interaction 1 (breast) (EPSTI1), transcript variant 1 |
CNY 3,656.00 |
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