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MARCHF8 (NM_001002266) Human Tagged ORF Clone
CAT＃: RC205477
MARCH8 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 7
ORF Plasmid: tGFP
"NM_001002266" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | c-MIR; CMIR; MARCH-VIII; MARCH8; MIR; RNF178 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205477 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATGCCACTGCATCAGATCTCTGCCATTCCATCCCAGGATGCCATCTCTGCTAGAGTCTACAGAA GTAAGACCAAAGAAAAGGAGAGGGAAGAACAGAATGAGAAGACTTTGGGACATTTCATGAGTCATTCAAG CAACATTTCTAAGGCTGGGAGTCCTCCGTCAGCATCAGCTCCGGCTCCGGTGTCCTCCTTCTCTCGCACT TCTATCACGCCATCCAGCCAGGACATCTGCAGGATCTGCCACTGTGAAGGAGATGATGAGAGCCCCCTGA TCACCCCCTGCCACTGCACAGGAAGCCTCCACTTCGTGCACCAGGCCTGCCTGCAGCAGTGGATCAAGAG CTCCGACACGCGCTGCTGCGAGCTCTGCAAGTATGAGTTCATCATGGAGACCAAGCTGAAGCCACTGAGA AAATGGGAGAAGTTGCAGATGACGTCCAGCGAGCGCAGGAAGATCATGTGCTCAGTGACATTCCACGTCA TTGCCATCACATGTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGTACTGCTGAGGAGATCAAGCAGGG GCAGGCAACAGGAATCCTAGAATGGCCCTTTTGGACTAAATTGGTGGTTGTGGCCATCGGCTTCACCGGA GGACTTCTTTTTATGTATGTTCAGTGTAAAGTGTATGTGCAATTGTGGAAGAGACTCAAGGCCTATAATA GAGTGATCTATGTTCAAAACTGTCCAGAAACAAGCAAAAAGAATATTTTTGAAAAATCTCCACTAACAGA GCCCAACTTTGAAAATAAACATGGACATGGAATCTGTCATTCCGACACAAACTCTTCTTGTTGCACAGAG CCTGAAGACACTGGAGCAGAAATCATTCACGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205477 protein sequence
Red=Cloning site Green=Tags(s) MSMPLHQISAIPSQDAISARVYRSKTKEKEREEQNEKTLGHFMSHSSNISKAGSPPSASAPAPVSSFSRT SITPSSQDICRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYEFIMETKLKPLR KWEKLQMTSSERRKIMCSVTFHVIAITCVVWSLYVLIDRTAEEIKQGQATGILEWPFWTKLVVVAIGFTG GLLFMYVQCKVYVQLWKRLKAYNRVIYVQNCPETSKKNIFEKSPLTEPNFENKHGHGICHSDTNSSCCTE PEDTGAEIIHV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001002266 |
| ORF Size | 873 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001002266.3 |
| RefSeq Size | 4812 bp |
| RefSeq ORF | 876 bp |
| Locus ID | 220972 |
| UniProt ID | Q5T0T0 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 32.9 kDa |
| Gene Summary | MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205477L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 7, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205477L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 7, mGFP tagged |
CNY 5,890.00 |
|
| RG205477 | 41341 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 7 |
CNY 4,000.00 |
|
| SC300430 | 41341 (untagged)-Human membrane-associated ring finger (C3HC4) 8 (MARCH8), transcript variant 7 |
CNY 2,640.00 |
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