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MIRO2 (RHOT2) (NM_138769) Human Tagged ORF Clone
CAT＃: RC204823
RHOT2 (Myc-DDK-tagged)-Human ras homolog gene family, member T2 (RHOT2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_138769" in other vectors (6)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARHT2; C16orf39; MIRO-2; MIRO2; RASL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204823 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGGGACGTGCGCATCCTGTTACTGGGCGAGGCCCAGGTGGGGAAGACGTCGCTGATCCTGTCCC TGGTGGGCGAGGAGTTCCCCGAGGAGGTCCCTCCCCGCGCGGAGGAGATCACGATCCCCGCGGACGTCAC CCCGGAGAAGGTGCCCACCCACATCGTGGACTACTCAGAAGCCGAGCAGACGGACGAGGAGCTGCGGGAG GAGATCCACAAGGCAAACGTGGTGTGTGTGGTGTATGACGTCTCTGAGGAGGCCACCATTGAGAAGATTC GAACTAAGTGGATCCCACTGGTGAATGGGGGGACCACGCAGGGGCCCAGGGTGCCCATCATCCTAGTGGG CAACAAGTCAGACCTGCGGTCGGGGAGCTCCATGGAGGCCGTGCTCCCCATCATGAGCCAGTTTCCCGAG ATTGAGACCTGCGTGGAGTGTTCGGCCAAGAACCTGAGGAACATCTCAGAGCTGTTCTACTACGCCCAGA AGGCCGTCCTGCATCCCACAGCCCCCCTCTATGACCCTGAGGCCAAGCAGTTGAGGCCCGCGTGCGCCCA GGCGCTGACGCGCATCTTCAGGCTCTCAGATCAGGACCTGGACCAGGCGCTCAGTGACGAAGAGCTCAAC GCTTTCCAGAAATCCTGCTTTGGGCACCCCCTGGCCCCGCAGGCCCTGGAGGACGTGAAGACGGTGGTGT GCAGGAACGTGGCGGGCGGCGTGCGGGAGGACCGGCTGACCCTGGATGGTTTCCTCTTCCTGAACACGCT CTTCATCCAGCGCGGCCGGCACGAGACCACCTGGACCATCCTGCGGCGCTTCGGCTACAGCGATGCCCTG GAGCTGACTGCGGACTATCTCTCCCCTCTGATCCACGTGCCCCCCGGCTGCAGCACGGAGCTCAACCACC TTGGCTACCAGTTTGTGCAGAGAGTGTTTGAGAAGCACGACCAGGACCGCGACGGCGCCCTCTCGCCCGT GGAGCTGCAAAGCCTTTTCAGTGTGTTCCCAGCAGCGCCCTGGGGCCCCGAGCTCCCACGCACAGTCCGC ACAGAGGCCGGCCGGTTGCCCCTGCACGGATACCTCTGCCAGTGGACCCTGGTGACCTACCTGGACGTCC GGAGCTGCCTTGGACACCTAGGCTACCTGGGCTACCCCACCCTCTGTGAGCAGGACCAGGCCCATGCCAT CACAGTCACTCGTGAGAAGAGGCTGGACCAGGAGAAGGGACAGACGCAGCGGAGCGTCCTCCTGTGCAAG GTGGTAGGGGCCCGTGGAGTGGGCAAGTCTGCCTTCCTGCAGGCTTTTCTCGGCCGCGGCCTGGGGCACC AGGACACGAGGGAGCAGCCTCCCGGCTACGCCATCGACACGGTGCAGGTCAATGGACAGGAGAAGTACTT GATCCTCTGTGAGGTGGGCACAGATGGTCTGCTGGCCACATCGCTGGACGCCACCTGTGACGTTGCCTGC TTGATGTTTGATGGCAGTGACCCAAAGTCCTTTGCACATTGTGCCAGCGTCTACAAGCACCATTACATGG ACGGGCAGACCCCCTGCCTCTTTGTCTCCTCCAAGGCCGACCTGCCCGAAGGTGTCGCGGTGTCTGGCCC ATCACCGGCCGAGTTTTGCCGCAAGCACCGGCTACCCGCTCCCGTGCCGTTCTCCTGTGCTGGCCCAGCC GAGCCCAGCACCACCATCTTCACCCAGCTCGCCACCATGGCCGCCTTCCCACATTTGGTCCACGCAGAGC TGCATCCCTCTTCCTTCTGGCTCCGGGGGCTGCTGGGGGTTGTCGGGGCCGCCGTGGCCGCAGTCCTCAG CTTCTCACTCTACAGGGTCCTGGTGAAGAGCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204823 protein sequence
Red=Cloning site Green=Tags(s) MRRDVRILLLGEAQVGKTSLILSLVGEEFPEEVPPRAEEITIPADVTPEKVPTHIVDYSEAEQTDEELRE EIHKANVVCVVYDVSEEATIEKIRTKWIPLVNGGTTQGPRVPIILVGNKSDLRSGSSMEAVLPIMSQFPE IETCVECSAKNLRNISELFYYAQKAVLHPTAPLYDPEAKQLRPACAQALTRIFRLSDQDLDQALSDEELN AFQKSCFGHPLAPQALEDVKTVVCRNVAGGVREDRLTLDGFLFLNTLFIQRGRHETTWTILRRFGYSDAL ELTADYLSPLIHVPPGCSTELNHLGYQFVQRVFEKHDQDRDGALSPVELQSLFSVFPAAPWGPELPRTVR TEAGRLPLHGYLCQWTLVTYLDVRSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQTQRSVLLCK VVGARGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILCEVGTDGLLATSLDATCDVAC LMFDGSDPKSFAHCASVYKHHYMDGQTPCLFVSSKADLPEGVAVSGPSPAEFCRKHRLPAPVPFSCAGPA EPSTTIFTQLATMAAFPHLVHAELHPSSFWLRGLLGVVGAAVAAVLSFSLYRVLVKSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138769 |
| ORF Size | 1854 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138769.1, NP_620124.1 |
| RefSeq Size | 2556 bp |
| RefSeq ORF | 1857 bp |
| Locus ID | 89941 |
| UniProt ID | Q8IXI1 |
| Domains | EFh, RAS, RHO, RAB |
| Protein Families | Transmembrane |
| MW | 68.1 kDa |
| Gene Summary | This gene encodes a member of the Rho family of GTPases. The encoded protein is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204823L1 | Lenti ORF clone of Human ras homolog gene family, member T2 (RHOT2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204823L2 | Lenti ORF clone of Human ras homolog gene family, member T2 (RHOT2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RC204823L3 | Lenti ORF clone of Human ras homolog gene family, member T2 (RHOT2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204823L4 | Lenti ORF clone of Human ras homolog gene family, member T2 (RHOT2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG204823 | RHOT2 (tGFP-tagged) - Human ras homolog gene family, member T2 (RHOT2), nuclear gene encoding mitochondrial protein |
CNY 6,200.00 |
|
| SC127208 | RHOT2 (untagged)-Human ras homolog gene family, member T2 (RHOT2), nuclear gene encoding mitochondrial protein |
CNY 2,950.00 |
