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COTL1 (NM_021149) Human Tagged ORF Clone
CAT＃: RC204055
COTL1 (Myc-DDK-tagged)-Human coactosin-like 1 (Dictyostelium) (COTL1)
ORF Plasmid: tGFP
"NM_021149" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
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CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CLP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204055 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCAAGATCGACAAAGAGGCTTGCCGGGCGGCGTACAACCTGGTGCGCGACGACGGCTCGGCCG TCATCTGGGTGACTTTTAAATATGACGGCTCCACCATCGTCCCCGGCGAGCAGGGAGCGGAGTACCAGCA CTTCATCCAGCAGTGCACAGATGACGTCCGGTTGTTTGCCTTCGTGCGCTTCACCACCGGGGATGCCATG AGCAAGAGGTCCAAGTTTGCCCTCATCACGTGGATCGGTGAGAACGTCAGCGGGCTGCAGCGCGCCAAAA CCGGGACGGACAAGACCCTGGTGAAGGAGGTCGTACAGAATTTCGCTAAGGAGTTTGTGATCAGTGATCG GAAGGAGCTGGAGGAAGATTTCATCAAGAGCGAGCTGAAGAAGGCGGGGGGAGCCAATTACGACGCCCAG ACGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204055 protein sequence
Red=Cloning site Green=Tags(s) MATKIDKEACRAAYNLVRDDGSAVIWVTFKYDGSTIVPGEQGAEYQHFIQQCTDDVRLFAFVRFTTGDAM SKRSKFALITWIGENVSGLQRAKTGTDKTLVKEVVQNFAKEFVISDRKELEEDFIKSELKKAGGANYDAQ TE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021149 |
| ORF Size | 426 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021149.5 |
| RefSeq Size | 1893 bp |
| RefSeq ORF | 429 bp |
| Locus ID | 23406 |
| UniProt ID | Q14019 |
| Domains | ADF |
| MW | 15.9 kDa |
| Gene Summary | This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204055L1 | Lenti ORF clone of Human coactosin-like 1 (Dictyostelium) (COTL1), Myc-DDK-tagged |
CNY 3,600.00 |
|
| RC204055L2 | Lenti ORF clone of Human coactosin-like 1 (Dictyostelium) (COTL1), mGFP tagged |
CNY 5,890.00 |
|
| RC204055L3 | Lenti ORF clone of Human coactosin-like 1 (Dictyostelium) (COTL1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204055L4 | Lenti ORF clone of Human coactosin-like 1 (Dictyostelium) (COTL1), mGFP tagged |
CNY 5,890.00 |
|
| RG204055 | COTL1 (tGFP-tagged) - Human coactosin-like 1 (Dictyostelium) (COTL1) |
CNY 2,800.00 |
|
| SC100289 | COTL1 (untagged)-Human coactosin-like 1 (Dictyostelium) (COTL1) |
CNY 1,200.00 |
