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PUS7 (NM_019042) Human Tagged ORF Clone
CAT＃: RC203902
PUS7 (Myc-DDK-tagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7)
ORF Plasmid: tGFP
"NM_019042" in other vectors (5)
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CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IDDABS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203902 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATGACAGAAATGACTGGTGTGTCGCTGAAACGTGGGGCACTGGTTGTCGAAGATAATGACAGTG GAGTCCCAGTTGAAGAGACAAAAAAACAGAAGCTGTCGGAATGCAGCCTAACCAAAGGTCAAGATGGGCT ACAGAATGACTTTCTGTCCATCAGTGAAGACGTGCCTCGGCCTCCTGACACTGTCAGTACTGGGAAAGGT GGAAAGAATTCTGAGGCTCAGTTGGAAGATGAGGAAGAAGAGGAGGAAGATGGACTTTCAGAGGAGTGCG AGGAGGAGGAATCAGAGAGTTTTGCAGACATGATGAAGCATGGACTCACTGAGGCTGACGTAGGCATCAC CAAGTTTGTGAGTTCTCATCAAGGGTTCTCGGGAATCTTAAAAGAAAGATACTCCGACTTCGTTGTTCAT GAAATAGGAAAAGATGGACGGATCAGCCATTTGAATGACTTGTCCATTCCAGTGGATGAGGAGGACCCTT CAGAAGACATATTTACAGTTTTGACAGCTGAAGAAAAGCAGCGATTGGAAGAGCTCCAGCTGTTCAAAAA TAAGGAAACCAGTGTTGCCATTGAGGTTATCGAGGACACCAAAGAGAAAAGAACCATCATCCATCAGGCT ATCAAATCTCTGTTTCCAGGATTAGAGACAAAAACAGAGGATAGGGAGGGGAAGAAATACATTGTAGCCT ACCACGCAGCTGGGAAAAAGGCTTTGGCAAATCCAAGAAAACATTCTTGGCCAAAATCTAGGGGAAGTTA CTGCCACTTCGTACTATATAAGGAAAACAAAGACACCATGGATGCTATTAATGTACTCTCCAAATACTTA AGAGTCAAGCCAAATATATTCTCCTACATGGGAACCAAAGATAAAAGGGCTATAACAGTTCAAGAAATTG CTGTTCTCAAAATAACTGCACAAAGACTTGCCCACCTGAATAAGTGCTTGATGAACTTTAAGCTAGGGAA TTTCAGCTATCAAAAAAACCCACTGAAATTGGGAGAGCTTCAAGGAAACCACTTCACTGTTGTTCTCAGA AATATAACAGGAACTGATGACCAAGTACAGCAAGCTATGAACTCTCTCAAGGAGATTGGATTTATTAACT ACTATGGAATGCAAAGATTTGGAACCACAGCTGTCCCTACGTATCAGGTTGGAAGAGCTATACTACAAAA TTCCTGGACAGAAGTCATGGATTTAATATTGAAACCCCGCTCTGGAGCTGAAAAGGGCTACTTGGTTAAA TGCAGAGAAGAATGGGCAAAGACCAAAGACCCAACTGCTGCCCTCAGAAAACTACCTGTCAAAAGGTGTG TGGAAGGGCAGCTGCTTCGAGGACTTTCAAAATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAAT ACCCAGAAATAATCGCTTAATGTATATTCATAGCTACCAAAGCTATGTGTGGAATAACATGGTAAGCAAG AGGATAGAAGACTATGGACTAAAACCTGTTCCAGGGGACCTCGTTCTCAAAGGAGCCACAGCCACCTATA TTGAGGAAGATGATGTTAATAATTACTCTATCCATGATGTGGTAATGCCCTTGCCTGGTTTCGATGTTAT CTACCCAAAGCATAAAATTCAAGAAGCCTACAGGGAAATGCTCACAGCTGACAATCTTGATATTGACAAC ATGAGACACAAAATTCGAGATTATTCCTTGTCAGGGGCCTACCGAAAGATCATTATTCGTCCTCAGAATG TTAGCTGGGAAGTCGTTGCATATGATGATCCCAAAATTCCACTTTTCAACACAGATGTGGACAACCTAGA AGGGAAGACACCACCAGTTTTTGCTTCTGAAGGCAAATACAGGGCTCTGAAAATGGATTTTTCTCTACCC CCTTCTACTTACGCCACCATGGCCATTCGAGAAGTGCTAAAAATGGATACCAGTATCAAGAACCAGACGC AGCTGAATACAACCTGGCTTCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203902 protein sequence
Red=Cloning site Green=Tags(s) MEMTEMTGVSLKRGALVVEDNDSGVPVEETKKQKLSECSLTKGQDGLQNDFLSISEDVPRPPDTVSTGKG GKNSEAQLEDEEEEEEDGLSEECEEEESESFADMMKHGLTEADVGITKFVSSHQGFSGILKERYSDFVVH EIGKDGRISHLNDLSIPVDEEDPSEDIFTVLTAEEKQRLEELQLFKNKETSVAIEVIEDTKEKRTIIHQA IKSLFPGLETKTEDREGKKYIVAYHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYL RVKPNIFSYMGTKDKRAITVQEIAVLKITAQRLAHLNKCLMNFKLGNFSYQKNPLKLGELQGNHFTVVLR NITGTDDQVQQAMNSLKEIGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSGAEKGYLVK CREEWAKTKDPTAALRKLPVKRCVEGQLLRGLSKYGMKNIVSAFGIIPRNNRLMYIHSYQSYVWNNMVSK RIEDYGLKPVPGDLVLKGATATYIEEDDVNNYSIHDVVMPLPGFDVIYPKHKIQEAYREMLTADNLDIDN MRHKIRDYSLSGAYRKIIIRPQNVSWEVVAYDDPKIPLFNTDVDNLEGKTPPVFASEGKYRALKMDFSLP PSTYATMAIREVLKMDTSIKNQTQLNTTWLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019042 |
| ORF Size | 1983 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019042.5 |
| RefSeq Size | 3484 bp |
| RefSeq ORF | 1986 bp |
| Locus ID | 54517 |
| UniProt ID | Q96PZ0 |
| MW | 75 kDa |
| Gene Summary | Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203902L3 | Lenti ORF clone of Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203902L4 | Lenti ORF clone of Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7), mGFP tagged |
CNY 5,890.00 |
|
| RG203902 | PUS7 (tGFP-tagged) - Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) |
CNY 4,370.00 |
|
| SC113305 | PUS7 (untagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) |
CNY 2,400.00 |
|
| SC318122 | PUS7 (untagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) |
CNY 7,220.00 |
