RING1 (NM_002931) Human Tagged ORF Clone

CAT#: RC202650

RING1 (Myc-DDK-tagged)-Human ring finger protein 1 (RING1)

ORF Plasmid: DDK tGFP



  "NM_002931" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


RING1 mouse monoclonal antibody,clone OTI3G7
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms RING1A; RNF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202650 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGACGCCGGCGAATGCCCAGAATGCCAGCAAAACGTGGGAACTGAGTCTGTATGAGCTGCACCGGA
CCCCGCAGGAAGCCATAATGGATGGCACAGAGATTGCTGTTTCCCCTCGGTCACTGCATTCAGAACTCAT
GTGCCCTATCTGCCTGGACATGCTGAAGAATACGATGACCACCAAGGAGTGCCTCCACAGATTCTGCTCT
GACTGCATTGTCACAGCCCTACGGAGCGGGAACAAGGAGTGTCCTACCTGCCGAAAGAAGCTGGTGTCCA
AGCGATCCCTACGGCCAGACCCCAACTTTGATGCCCTGATCTCTAAGATCTATCCTAGCCGGGAGGAATA
CGAGGCCCATCAAGACCGAGTGCTTATCCGCCTGAGCCGCCTGCACAACCAGCAGGCATTGAGCTCCAGC
ATTGAGGAGGGGCTACGCATGCAGGCCATGCACAGGGCCCAGCGTGTGAGGCGGCCGATACCAGGGTCAG
ATCAGACCACAACGATGAGTGGGGGGGAAGGAGAGCCCGGGGAGGGAGAAGGGGATGGAGAAGATGTGAG
CTCAGACTCCGCCCCTGACTCTGCCCCAGGCCCTGCTCCCAAGCGACCCCGTGGAGGGGGCGCAGGGGGG
AGCAGTGTAGGGACAGGGGGAGGCGGCACTGGTGGGGTGGGTGGGGGTGCCGGTTCGGAAGACTCTGGTG
ACCGGGGAGGGACTCTGGGAGGGGGAACGCTGGGCCCCCCAAGCCCTCCTGGGGCCCCCAGCCCCCCAGA
GCCAGGTGGAGAAATTGAGCTCGTGTTCCGGCCCCACCCCCTGCTCGTGGAGAAGGGAGAATACTGCCAG
ACGAGGTATGTGAAGACAACTGGGAATGCCACAGTGGACCACCTCTCCAAGTACTTGGCCCTGCGCATTG
CCCTCGAGCGGAGGCAACAGCAGGAAGCAGGGGAGCCAGGAGGGCCTGGAGGGGGCGCCTCTGACACCGG
AGGACCTGATGGGTGTGGCGGGGAGGGTGGGGGTGCCGGAGGAGGTGACGGTCCTGAGGAGCCTGCTTTG
CCCAGCCTGGAGGGCGTCAGTGAAAAGCAGTACACCATCTACATCGCACCTGGAGGCGGGGCGTTCACGA
CGTTGAATGGCTCGCTGACCCTGGAGCTGGTGAATGAGAAATTCTGGAAGGTGTCCCGGCCACTGGAGCT
GTGCTATGCTCCCACCAAGGATCCAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202650 protein sequence
Red=Cloning site Green=Tags(s)

MTTPANAQNASKTWELSLYELHRTPQEAIMDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCS
DCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSS
IEEGLRMQAMHRAQRVRRPIPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGG
SSVGTGGGGTGGVGGGAGSEDSGDRGGTLGGGTLGPPSPPGAPSPPEPGGEIELVFRPHPLLVEKGEYCQ
TRYVKTTGNATVDHLSKYLALRIALERRQQQEAGEPGGPGGGASDTGGPDGCGGEGGGAGGGDGPEEPAL
PSLEGVSEKQYTIYIAPGGGAFTTLNGSLTLELVNEKFWKVSRPLELCYAPTKDPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002931
ORF Size 1218 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002931.4
RefSeq Size 1744 bp
RefSeq ORF 1221 bp
Locus ID 6015
UniProt ID Q06587
Domains RING
Protein Families Druggable Genome, Transcription Factors
MW 42.4 kDa
Gene Summary This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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