C18orf55 (TIMM21) (NM_014177) Human Tagged ORF Clone

CAT#: RC201470

  • TrueORF®

TIMM21 (Myc-DDK-tagged)-Human chromosome 18 open reading frame 55 (C18orf55), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP



  "NM_014177" in other vectors (7)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


TIMM21 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms C18orf55; HSPC154; TIM21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201470 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTTGTACTTTTCTACGAGCCGTACAGTATACGGAGAAGCTGCACAGGTCCTCGGCAAAGCGATTGC
TTTTGCCATACATCGTGCTTAACAAAGCGTGCTTGAAGACTGAGCCCAGTTTGAGATGTGGGCTTCAATA
TCAAAAGAAAACGCTGCGACCTAGATGTATTCTTGGAGTCACCCAGAAAACCATCTGGACGCAGGGACCG
AGCCCCCGAAAAGCAAAGGAGGATGGCAGCAAACAAGTGTCTGTGCACAGGAGTCAGAGAGGGGGAACCG
CCGTCCCAACATCACAAAAAGTGAAAGAAGCCGGAAGAGATTTTACCTATTTAATAGTGGTGCTTTTTGG
AATCAGCATTACAGGTGGCTTGTTTTACACGATTTTCAAAGAACTTTTTTCTTCATCCAGTCCTAGCAAG
ATATATGGGAGAGCCTTAGAAAAATGCAGATCACATCCTGAGGTGATCGGTGTCTTTGGTGAGTCTGTTA
AAGGCTATGGGGAGGTGACAAGGCGGGGTCGCCGGCAGCATGTCAGGTTCACTGAATATGTAAAAGATGG
GCTGAAACACACGTGTGTGAAATTCTACATTGAGGGCTCTGAGCCAGGGAAGCAAGGAACGGTGTATGCG
CAAGTGAAAGAGAACCCAGGAAGTGGTGAATATGATTTTCGATATATATTTGTAGAAATTGAATCTTATC
CTAGAAGAACTATTATCATTGAAGATAATCGATCCCAAGATGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201470 protein sequence
Red=Cloning site Green=Tags(s)

MICTFLRAVQYTEKLHRSSAKRLLLPYIVLNKACLKTEPSLRCGLQYQKKTLRPRCILGVTQKTIWTQGP
SPRKAKEDGSKQVSVHRSQRGGTAVPTSQKVKEAGRDFTYLIVVLFGISITGGLFYTIFKELFSSSSPSK
IYGRALEKCRSHPEVIGVFGESVKGYGEVTRRGRRQHVRFTEYVKDGLKHTCVKFYIEGSEPGKQGTVYA
QVKENPGSGEYDFRYIFVEIESYPRRTIIIEDNRSQDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014177
ORF Size 744 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014177.3
RefSeq Size 1562 bp
RefSeq ORF 747 bp
Locus ID 29090
UniProt ID Q9BVV7
Protein Families Transmembrane
MW 28.2 kDa
Gene Summary Participates in the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Also required for assembly of mitochondrial respiratory chain complex I and complex IV as component of the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complex) complex. Probably shuttles between the presequence translocase and respiratory-chain assembly intermediates in a process that promotes incorporation of early nuclear-encoded subunits into these complexes.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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