C3orf10 (BRK1) (NM_018462) Human Tagged ORF Clone
CAT#: RC200804
BRK1 (Myc-DDK-tagged)-Human chromosome 3 open reading frame 10 (C3orf10)
ORF Plasmid: tGFP
"NM_018462" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C3orf10; hHBrk1; HSPC300; MDS027 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200804 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGACAGGAGGATCCGGTGCAGCGGGAGATTCACCAGGACTGGGCTAACCGGGAGTACATTGAGA TAATCACCAGCAGCATCAAGAAAATCGCAGACTTTCTCAACTCGTTCGATATGTCTTGTCGTTCAAGACT TGCAACACTAAACGAGAAATTGACAGCCCTTGAACGGAGAATAGAGTACATTGAAGCTCGGGTGACAAAA GGTGAGACACTCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200804 protein sequence
Red=Cloning site Green=Tags(s) MAGQEDPVQREIHQDWANREYIEIITSSIKKIADFLNSFDMSCRSRLATLNEKLTALERRIEYIEARVTK GETLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_018462 |
ORF Size | 225 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018462.5 |
RefSeq Size | 1197 bp |
RefSeq ORF | 228 bp |
Locus ID | 55845 |
UniProt ID | Q8WUW1 |
Protein Pathways | Regulation of actin cytoskeleton |
MW | 8.7 kDa |
Gene Summary | Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200804L3 | Lenti ORF clone of Human chromosome 3 open reading frame 10 (C3orf10), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200804L4 | Lenti ORF clone of Human chromosome 3 open reading frame 10 (C3orf10), mGFP tagged |
CNY 5,890.00 |
|
RG200804 | BRK1 (tGFP-tagged) - Human chromosome 3 open reading frame 10 (C3orf10) |
CNY 2,800.00 |
|
SC111347 | BRK1 (untagged)-Human chromosome 3 open reading frame 10 (C3orf10) |
CNY 1,200.00 |
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