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HYAL2 (NM_033158) Human Tagged ORF Clone
CAT＃: RC200552
HYAL2 (Myc-DDK-tagged)-Human hyaluronoglucosaminidase 2 (HYAL2), transcript variant 2
ORF Plasmid: tGFP
"NM_033158" in other vectors (6)
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CNY 3,656.00
CNY 5,320.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LUCA2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200552 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCAGGCCCAGGCCCCACCGTTACATTGGCCCTGGTGCTGGCGGTGGCATGGGCCATGGAGCTCA AGCCCACAGCACCACCCATCTTCACTGGCCGGCCCTTTGTGGTAGCGTGGGACGTGCCCACACAGGACTG TGGCCCACGCCTCAAGGTGCCACTGGACCTGAATGCCTTTGATGTGCAGGCCTCACCTAATGAGGGTTTT GTGAACCAGAATATTACCATCTTCTACCGCGACCGTCTAGGCCTGTATCCACGCTTCGATTCTGCCGGAA GGTCTGTGCATGGTGGTGTGCCACAGAATGTCAGCCTTTGGGCACACCGGAAGATGCTGCAGAAACGTGT GGAGCACTACATTCGGACACAGGAGTCTGCGGGGCTGGCGGTCATCGACTGGGAGGACTGGCGACCTGTG TGGGTGCGCAACTGGCAGGACAAAGATGTGTATCGCCGGTTATCACGCCAGCTAGTGGCCAGTCGTCACC CTGACTGGCCTCCAGACCGCATAGTCAAACAGGCACAATATGAGTTTGAGTTCGCAGCACAGCAGTTCAT GCTGGAGACACTGCGTTATGTCAAGGCAGTGCGGCCCCGGCACCTCTGGGGCTTCTACCTCTTTCCTGAC TGCTACAATCATGATTATGTGCAGAACTGGGAGAGCTACACAGGCCGCTGCCCTGATGTTGAGGTGGCCC GCAATGACCAGCTGGCCTGGCTGTGGGCTGAGAGCACGGCCCTCTTCCCGTCTGTCTACCTGGACGAGAC ACTTGCTTCCTCCCGCCATGGCCGCAACTTTGTGAGCTTCCGTGTTCAGGAGGCCCTTCGTGTGGCTCGC ACCCACCATGCCAACCATGCACTCCCAGTCTACGTCTTCACACGACCCACCTACAGCCGCAGGCTCACGG GGCTTAGTGAGATGGACCTCATCTCTACCATTGGCGAGAGTGCGGCCCTGGGCGCAGCTGGTGTCATCCT CTGGGGTGACGCGGGGTACACCACAAGCACGGAGACCTGCCAGTACCTCAAAGATTACCTGACACGGCTG CTGGTCCCCTACGTGGTCAATGTGTCCTGGGCCACCCAATATTGCAGCCGGGCCCAGTGCCATGGCCATG GGCGCTGTGTGCGCCGCAACCCCAGTGCCAGTACCTTCCTGCATCTCAGCACCAACAGTTTCCGCCTAGT GCCTGGCCATGCACCTGGTGAACCCCAGCTGCGACCTGTGGGGGAGCTCAGTTGGGCCGACATTGACCAC CTGCAGACACACTTCCGCTGCCAGTGCTACTTGGGCTGGAGTGGTGAGCAATGCCAGTGGGACCATAGGC AGGCAGCTGGAGGTGCCAGCGAGGCCTGGGCTGGGTCCCACCTCACCAGTCTGCTGGCTCTGGCAGCCCT GGCCTTTACCTGGACCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200552 protein sequence
Red=Cloning site Green=Tags(s) MRAGPGPTVTLALVLAVAWAMELKPTAPPIFTGRPFVVAWDVPTQDCGPRLKVPLDLNAFDVQASPNEGF VNQNITIFYRDRLGLYPRFDSAGRSVHGGVPQNVSLWAHRKMLQKRVEHYIRTQESAGLAVIDWEDWRPV WVRNWQDKDVYRRLSRQLVASRHPDWPPDRIVKQAQYEFEFAAQQFMLETLRYVKAVRPRHLWGFYLFPD CYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSRHGRNFVSFRVQEALRVAR THHANHALPVYVFTRPTYSRRLTGLSEMDLISTIGESAALGAAGVILWGDAGYTTSTETCQYLKDYLTRL LVPYVVNVSWATQYCSRAQCHGHGRCVRRNPSASTFLHLSTNSFRLVPGHAPGEPQLRPVGELSWADIDH LQTHFRCQCYLGWSGEQCQWDHRQAAGGASEAWAGSHLTSLLALAALAFTWTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033158 |
| ORF Size | 1419 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033158.1 |
| RefSeq Size | 2413 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 8692 |
| UniProt ID | Q12891 |
| Domains | Glyco_hydro_56 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycosaminoglycan degradation, Metabolic pathways |
| MW | 53.8 kDa |
| Gene Summary | This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR.[provided by RefSeq, Mar 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The suppressive role of HYAL1 and HYAL2 in the metastasis of colorectal cancer
,Jin, Z;Zhang, G;Liu, Y;He, Y;Yang, C;Du, Y;Gao, F;,
Journal of Gastroenterology and Hepatology
,PubMed ID 30972813
[HYAL2]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200552L1 | Lenti ORF clone of Human hyaluronoglucosaminidase 2 (HYAL2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200552L2 | Lenti ORF clone of Human hyaluronoglucosaminidase 2 (HYAL2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC200552L3 | Lenti ORF clone of Human hyaluronoglucosaminidase 2 (HYAL2), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200552L4 | Lenti ORF clone of Human hyaluronoglucosaminidase 2 (HYAL2), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG200552 | HYAL2 (tGFP-tagged) - Human hyaluronoglucosaminidase 2 (HYAL2), transcript variant 2 |
CNY 4,370.00 |
|
| SC321575 | HYAL2 (untagged)-Human hyaluronoglucosaminidase 2 (HYAL2), transcript variant 2 |
CNY 2,950.00 |
