Tcerg1 (NM_001289526) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2410022J09Rik; 2900090C16Rik; AI428505; ca150; CA150b; FBP23; FBP28; p144; Taf2s
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231578 representing NM_001289526 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCGTGGCGGGGACGGAGGGGAAGGCGAGCGGTTCAACCCGGGGGAGCTGAGGATGGCTCAAC AGCAGGCCTTGAGGTTCCGAGGCCCAGCTCCCCCACCAAATGCCGTGATGCGAGGCCCGCCACCTCTCAT GAGACCACCTCCTCCTTTCGGCATGATGAGAGGCCCTCCTCCACCACCCAGGCCCCCCTTTGGACGGCCT CCTTTTGATCCTAATATGCCACCAATGCCTCCTCCAGGAGGAATACCTCCACCTATGGGCCCACCTCACC TACAGAGACCACCTTTCATGCCTCCACCCATGGGAGCTATGCCTCCTCCTCCGGGTATGATGTTCCCACC AGGAATGCCTCCGGGGACTGCTCCTGGTGCTCCAGCACTGCCTCCCACTGAGGAGATATGGGTAGAAAAT AAAACTCCAGATGGGAAGGTTTATTACTACAATGCTCGGACTCGTGAATCTGCATGGACCAAGCCAGATG GAGTTAAGGTTATTCAGCAATCAGAACTGACGCCCATGCTTGCAGCCCAAGCACAGGTTCAGGCCCAGGC CCAGGCCCAGGCCCAAGCACAGGCTCAGGCCCAGGCCCAGGCACAAGCCCAGGCTCAGGCCCAGGCCCAG GCCCAGGCTCAGGCACAAGCCCAGGCTCAGGCCCAGGCCCAGGCCCAGGCTCAGGCTCAAGCCCAAGCCC AAGCGCAAGCACAAGCCCAGGCCCAAGCCCAGGCCCAGGCTCAGGCCCAGGCACAAGTCCAAGCACAAGC AGTAGGCGCTCCCACCCCTACCACCAGTAGCCCAGCACCTGCAGTGTCCACGTCAACTCCGACATCTACC CCATCCTCCACCACAGCTACCACGACAACTGCTACTTCAGTTGCACAGACAGTATCAACACCCACAACAC AAGACCAGACCCCAAGTTCTGCTGTTTCAGTTGCCACACCTACAGTTAGTGTTTCAGCTCCTGCTCCTAC AGCCACACCTGTGCAAACCGTACCCCAGCCGCACCCACAGACGTTACCTCCTGCTGTTCCTCACTCGGTA CCTCAGCCGGCAGCAGCAATACCTGCTTTCCCACCAGTAATGGTGCCTCCGTTCCGTGTGCCCCTGCCTG GCATGCCAATCCCACTTCCAGGTGTACTACCAGGAATGGCCCCTCCTATAGTACCCATGATCCATCCCCA GGTTGCTATTGCAGCTTCACCTGCTACCCTAGCTGGGGCAACAGCAGTTTCTGAGTGGACTGAATATAAA ACAGCAGATGGGAAGACGTACTATTATAATAATAGAACACTAGAATCCACGTGGGAGAAGCCCCAAGAAC TGAAGGAGAAAGAAAAATTAGATGAGAAGATTAAAGAACCAATTAAGGAGGCCTCTGAAGAGCCATTGCC AATGGAGACTGAGGAGGAAGATCCTAAGGAAGAGCCTGTGAAGGAGATAAAGGAGGAGCCCAAAGAAGAA GAGATGACTGAAGAAGAAAAGGCTGCCCAGAAGGCAAAGCCAGTAGCTACTACTCCTATTCCTGGTACTC CATGGTGTGTTGTTTGGACTGGTGATGAGAGGGTCTTCTTTTACAATCCCACCACCCGCCTTTCTATGTG GGACCGGCCTGATGATTTGATTGGAAGGGCGGATGTTGACAAAATTATTCAAGAGCCCCCTCATAAGAAA GGATTGGAAGACATGAAGAAGCTAAGGCACCCGGCTCCGACAATGCTGTCCATCCAAAAGTGGCAGTTCT CAATGAGCGCAATTAAAGAAGAACAAGAGTTAATGGAAGAAATGAATGAAGATGAGCCTATTAAAGCAAA AAAGCGGAAGAGAATGTCAAAGAAGTCCTTTATGTGGATTGCCCGAGCTTCTCTATTTAGGAGAGATGAT AATAAAGACATTGATTCAGAGAAAGAAGCTGCCATGGAAGCTGAAATTAAAGCTGCCCGAGAAAGGGCCA TTGTCCCTCTGGAGGCTCGAATGAAGCAGTTCAAGGACATGCTGCTAGAGAGAGGGGTGTCTGCTTTTTC AACATGGGAGAAAGAGTTGCACAAGATAGTATTTGACCCTCGGTACTTGCTTCTCAATCCTAAAGAAAGA AAACAGGTGTTTGATCAGTATGTCAAGACCAGAGCAGAGGAAGAACGCAGGGAAAAGAAAAACAAAATAA TGCAAGCCAAGGAAGATTTCAAAAAAATGATGGAAGAAGCAAAGTTTAATCCAAGAGCTACTTTTAGTGA ATTTGCTGCCAAACATGCTAAAGATTCAAGATTCAAAGCAATTGAAAAGATGAAAGATAGAGAAGCATTG TTTAATGAGTTTGTGGCAGCTGCAAGGAAGAAAGAAAAAGAAGATTCGAAGACGAGAGGGGAGAAGATTA AATCAGATTTCTTTGAACTGTTATCTAATCATCACTTGGATAGTCAATCTCGATGGAGCAAAGTAAAAGA CAAAGTAGAAAGTGACCCCCGATATAAAGCGGTGGATAGTTCATCAATGAGAGAAGACCTTTTCAAACAG TACATCGAGAAAATAGCCAAGAATTTAGACTCAGAAAAAGAAAAAGAGCTGGAGAGGCAAGCCCGCATTG AGGCAAGCCTTCGAGAACGGGAAAGAGAAGTTCAGAAGGCGCGCTCTGAGCAGACGAAAGAGATCGATCG GGAGAGAGAGCAGCACAAACGGGAGGAGGCTATCCAGAACTTCAAGGCTCTTCTGTCTGACATGGTACGT TCTTCAGATGTGTCCTGGTCTGATACTAGGAGGACCCTCCGGAAAGACCACCGCTGGGAATCTGGGTCCT TATTGGAAAGAGAGGAGAAAGAGAAACTTTTTAATGAACATATTGAAGCGCTTACCAAAAAGAAAAGGGA GCACTTTAGGCAGCTTCTGGACGAAACCTCTGCGATTACCTTAACATCCACGTGGAAAGAAGTGAAAAAA ATAATTAAGGAAGATCCTCGGTGCATTAAGTTCTCCTCCAGTGACAGGAAAAAACAAAGGGAGTTTGAAG AATACATCAGAGACAAATACATCACTGCCAAAGCTGACTTCAGGACACTTTTGAAAGAGACCAAATTCAT TACATACAGATCCAAAAAGTTAATCCAAGAATCTGATCAGCACCTGAAAGATGTAGAAAAGATTTTGCAG AATGACAAACGGTATCTAGTACTGGACTGTGTCCCCGAGGAGAGGAGGAAACTAATTGTGGCCTATGTTG ATGACCTGGATCGCCGAGGTCCACCCCCACCTCCCACAGCATCAGAGCCCACTAGACGATCAACAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231578 representing NM_001289526 Red=Cloning site Green=Tags(s) MAERGGDGGEGERFNPGELRMAQQQALRFRGPAPPPNAVMRGPPPLMRPPPPFGMMRGPPPPPRPPFGRP PFDPNMPPMPPPGGIPPPMGPPHLQRPPFMPPPMGAMPPPPGMMFPPGMPPGTAPGAPALPPTEEIWVEN KTPDGKVYYYNARTRESAWTKPDGVKVIQQSELTPMLAAQAQVQAQAQAQAQAQAQAQAQAQAQAQAQAQ AQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQAQVQAQAVGAPTPTTSSPAPAVSTSTPTST PSSTTATTTTATSVAQTVSTPTTQDQTPSSAVSVATPTVSVSAPAPTATPVQTVPQPHPQTLPPAVPHSV PQPAAAIPAFPPVMVPPFRVPLPGMPIPLPGVLPGMAPPIVPMIHPQVAIAASPATLAGATAVSEWTEYK TADGKTYYYNNRTLESTWEKPQELKEKEKLDEKIKEPIKEASEEPLPMETEEEDPKEEPVKEIKEEPKEE EMTEEEKAAQKAKPVATTPIPGTPWCVVWTGDERVFFYNPTTRLSMWDRPDDLIGRADVDKIIQEPPHKK GLEDMKKLRHPAPTMLSIQKWQFSMSAIKEEQELMEEMNEDEPIKAKKRKRMSKKSFMWIARASLFRRDD NKDIDSEKEAAMEAEIKAARERAIVPLEARMKQFKDMLLERGVSAFSTWEKELHKIVFDPRYLLLNPKER KQVFDQYVKTRAEEERREKKNKIMQAKEDFKKMMEEAKFNPRATFSEFAAKHAKDSRFKAIEKMKDREAL FNEFVAAARKKEKEDSKTRGEKIKSDFFELLSNHHLDSQSRWSKVKDKVESDPRYKAVDSSSMREDLFKQ YIEKIAKNLDSEKEKELERQARIEASLREREREVQKARSEQTKEIDREREQHKREEAIQNFKALLSDMVR SSDVSWSDTRRTLRKDHRWESGSLLEREEKEKLFNEHIEALTKKKREHFRQLLDETSAITLTSTWKEVKK IIKEDPRCIKFSSSDRKKQREFEEYIRDKYITAKADFRTLLKETKFITYRSKKLIQESDQHLKDVEKILQ NDKRYLVLDCVPEERRKLIVAYVDDLDRRGPPPPPTASEPTRRSTK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289526
ORF Size	3288 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289526.1, NP_001276455.1
RefSeq Size	4397 bp
RefSeq ORF	3291 bp
Locus ID	56070
UniProt ID	Q8CGF7
MW	124.1 kDa
Gene Summary	Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]

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