Adgrg2 (NM_001290446) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW212196; B830041D06Rik; Gpr64; Me6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231475 representing NM_001290446 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTTCTCTGGTGGGCAGTACAGCCCTGTTGGCAGACCTGAAGAGGTTTTACTGATATACAAGATAT TCCTTGTCATCATTTGTTTTCATGTCATTCTCGTTACATCCCTGAAAGAAAACGGTAATTCCAGTTTGTT ATCACCATCTGCTGAATCATCTCTTGTCAGTCTCATCCCCTACTCCAATGATGCTGCTTCAGAAGTTTTG TCGACTTTAAACAAAACAGAAAAATCTAAAATCACTATAGTAAAAACCTTCAATGCATCAGGAGTCAAAT CCCAGAGAAATATCTGCAATTTGTCATCTCTTTGCAATGACTCAGTATTTTTTAGAGGTGAGATAGTGTT TCAACATGATGAAGACCACAATGTTACCCAGAATCAAGATACAGCTAATGGCACCTTCGCTGGAGTCCTG TCTCTAAGTGAACTGAAGCGATCAGAGCTCAACAAAACTCTACAGACCTTAAGTGAGACTTACTTTATAG TGTGTGCTACAGCAGAGGCCCAAAGCACGGTAAACTGTACATTCACAGTAAAACTCAATGAGACCATGAA TGTGTGTGCCATGATGGTTACTTTCCAAACTGTACAGATTCGGCCAATGGAACAGTGCTGCTGTTCCCCG AGGACTCCCTGCCCTTCCTCACCAGAAGAGTTAGAAAAACTACAGTGTGAACTGCAGGATCCCATTGTCT GTCTTGCTGATCAACCGCATGGCCCACCGTTATCGTCTTCCAGCAAGCCTGTTGTACCTCAGGCCACCAT TATTTCCCATGTTGCTAGTGACTTCTCTTTGGCTGAACCCCTTGATCATGCCCTTATGACCCCAAGCACA CCCTCTCTGACACAAGAAAGTAACCTTCCATCTCCTCAGCCTACGATCCCCCTGGCTTCCAGTCCTGCCA CTGACTTGCCAGTTCAATCTGTAGTGGTCTCTTCTTTGCCTCAAACTGATCTTTCCCACACCCTGTCACC GGTGCAGTCCTCCATTCCCTCTCCTACCACACCAGCCCCATCTGTCCCTACAGAACTGGTCACCATCAGC ACACCTCCTGGTGAGACAGTTGTCAACACTAGCACTGTTTCTGATCTGGAAGCCCAAGTATCCCAGATGG AGAAAGCTTTGTCTTTGGGTAGCTTAGAGCCTAATCTTGCAGGCGAAATGGTAAACCGAGTCAGCAAACT CCTTCACTCTCCACCTGCCTTGCTAGCCCCTCTAGCTCAAAGGTTGCTAAAAGTGGTAGATGCCATTGGC TTACAGCTGAATTTTTCATCTACAACTATCAGTCTAACTTCACCTTCTTTGGCTCTTGCTGTGATCAGAG TGAATGCCAGTAATTTCAATACCACGACTTTTGCAGCCCAAGACCCAACAAATCTCCAGGTTTCTCTGGA AACCCCACCTCCTGAGAATAGTATTGGTGCCATTACTCTGCCCTCATCACTGATGAATAATTTGCCAGCT AATGATGTAGAATTGGCTTCAAGGATTCAGTTCAATTTCTTTGAAACACCCGCCCTGTTTCAGGATCCTT CCCTGGAGAACCTCACTCTGATAAGCTATGTCATATCATCAAGTGTCACAAACATGACCATCAAGAACTT GACAAGAAACGTGACAGTCGCACTGAAACACATCAACCCAAGTCCGGATGACTTAACTGTGAAATGTGTA TTCTGGGACTTGGGCAGAAATGGTGGCAAAGGAGGTTGGTCATCTGATGGCTGTTCCGTCAAAGACAAGA GAATGAATGAAACCATCTGTACCTGTAGCCATCTTACAAGTTTTGGCATCCTATTGGACCTATCTCGAAC ATCCTTACCACCAAGTCAAATGATGGCTCTGACATTTATCACGTATATTGGCTGTGGGCTTTCATCAATT TTTCTGTCAGTTACTCTTGTAACCTACATCGCCTTTGAAAAGATCCGGAGGGATTACCCCTCCAAAATCC TCATCCAGCTGTGTGCTGCCCTGCTTCTGCTCAACCTGATCTTCCTCCTAGACTCCTGGATTGCGCTGTA TAATACCCGAGGTTTCTGCATTGCCGTGGCTGTATTTCTTCACTATTTTCTCTTGGTCTCATTCACATGG ATGGGATTAGAAGCATTCCACATGTACCTAGCACTGGTCAAGGTGTTTAATACTTACATCCGAAAGTACA TCCTTAAATTCTGCATTGTTGGCTGGGGCATACCAGCTGTGGTTGTGTCCATCGTCCTGACTATATCCCC AGATAACTATGGGATTGGATCCTATGGAAAATTCCCCAATGGCACACCAGATGACTTTTGCTGGATCAAC AGCAATGTGGTGTTCTATATCACGGTTGTGGGATATTTCTGTGTGATATTTCTACTGAACGTCAGCATGT TCATCGTGGTCTTGGTTCAGCTCTGTCGAATTAAAAAGAAGAAGCAGCTGGGAGCCCAGCGCAAAACTAG TATTCAAGACCTCAGGAGTATCGCTGGCCTCACATTTTTACTGGGAATTACTTGGGGCTTTGCCTTCTTT GCCTGGGGACCAGTTAATGTCACCTTCATGTATCTCTTTGCCATCTTTAACACCTTACAAGGGTTTTTCA TATTCATTTTTTACTGTGCAGCAAAAGAGAATGTCAGAAAACAGTGGAGGCGGTATCTTTGTTGTGGAAA ATTACGGCTGGCTGAAAATTCTGACTGGAGTAAAACTGCTACTAATGGTTTAAAGAAGCAGACTGTAAAC CAAGGAGTATCCAGCTCTTCAAATTCCTTACAGTCAAGCTGTAACTCCACTAACTCCACCACACTCCTAG TGAATAGTGATTGCTCAGTGCACGCAAGCGGGAATGGCAATGCATCTACCGAGAGGAATGGGGTTTCTTT CAGTGTTCAGAATGGAGACGTGTGCCTTCATGATCTCACTGGCAAACAGCATATGTTTAGTGACAAAGAG GACTCCTGCAATGGTAAAAGCCGGATAGCGCTCAGAAGGACTTCGAAGCGGGGAAGCTTACACTTTATCG AGCAAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231475 representing NM_001290446 Red=Cloning site Green=Tags(s) MLFSGGQYSPVGRPEEVLLIYKIFLVIICFHVILVTSLKENGNSSLLSPSAESSLVSLIPYSNDAASEVL STLNKTEKSKITIVKTFNASGVKSQRNICNLSSLCNDSVFFRGEIVFQHDEDHNVTQNQDTANGTFAGVL SLSELKRSELNKTLQTLSETYFIVCATAEAQSTVNCTFTVKLNETMNVCAMMVTFQTVQIRPMEQCCCSP RTPCPSSPEELEKLQCELQDPIVCLADQPHGPPLSSSSKPVVPQATIISHVASDFSLAEPLDHALMTPST PSLTQESNLPSPQPTIPLASSPATDLPVQSVVVSSLPQTDLSHTLSPVQSSIPSPTTPAPSVPTELVTIS TPPGETVVNTSTVSDLEAQVSQMEKALSLGSLEPNLAGEMVNRVSKLLHSPPALLAPLAQRLLKVVDAIG LQLNFSSTTISLTSPSLALAVIRVNASNFNTTTFAAQDPTNLQVSLETPPPENSIGAITLPSSLMNNLPA NDVELASRIQFNFFETPALFQDPSLENLTLISYVISSSVTNMTIKNLTRNVTVALKHINPSPDDLTVKCV FWDLGRNGGKGGWSSDGCSVKDKRMNETICTCSHLTSFGILLDLSRTSLPPSQMMALTFITYIGCGLSSI FLSVTLVTYIAFEKIRRDYPSKILIQLCAALLLLNLIFLLDSWIALYNTRGFCIAVAVFLHYFLLVSFTW MGLEAFHMYLALVKVFNTYIRKYILKFCIVGWGIPAVVVSIVLTISPDNYGIGSYGKFPNGTPDDFCWIN SNVVFYITVVGYFCVIFLLNVSMFIVVLVQLCRIKKKKQLGAQRKTSIQDLRSIAGLTFLLGITWGFAFF AWGPVNVTFMYLFAIFNTLQGFFIFIFYCAAKENVRKQWRRYLCCGKLRLAENSDWSKTATNGLKKQTVN QGVSSSSNSLQSSCNSTNSTTLLVNSDCSVHASGNGNASTERNGVSFSVQNGDVCLHDLTGKQHMFSDKE DSCNGKSRIALRRTSKRGSLHFIEQM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290446
ORF Size	3018 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290446.1, NP_001277375.1
RefSeq Size	4714 bp
RefSeq ORF	3021 bp
Locus ID	237175
UniProt ID	Q8CJ12
MW	110.4 kDa
Gene Summary	Orphan receptor. Could be involved in a signal transduction pathway controlling epididymal function and male fertility. May regulate fluid exchange within epididymis.[UniProtKB/Swiss-Prot Function]

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