Bmpr1b (NM_001277220) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Acv; Acvrlk6; AI385617; Al; ALK-6; Alk6; AV355320; BMPR; BMPR-1B; BMPR-IB; CFK-43; CFK-43a; SKR6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230201 representing NM_001277220 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGCACCAAGAAGGAGGATGGAGAGAGTACAGCCCCCACCCCTCGGCCCAAGATCCTACGTTGTAA ATGCCACCACCACTGTCCGGAAGACTCAGTCAACAATATCTGCAGATGGGTACTGCTTCACGATGATAGA AGAAGATGACTCTGGAATGCCTGTTGTCACCTCTGGATGTCTAGGACTAGAAGGGTCAGATTTTCAATGT CGTGACACTCCCATTCCTCATCAAAGAAGATCAATTGAATGCTGCACAGAAAGGAATGAGTGTAATAAAG ACCTCCACCCCACTCTGCCTCCTCTCAAGGACAGAGATTTTGTTGATGGGCCCATACACCACAAGGCCTT GCTTATCTCTGTGACTGTCTGTAGTTTACTCTTGGTCCTCATTATTTTATTCTGTTACTTCAGGTATAAA AGACAAGAAGCCCGACCTCGGTACAGCATTGGGCTGGAGCAGGACGAGACATACATTCCTCCTGGAGAGT CCCTGAGAGACTTGATCGAGCAGTCTCAGAGCTCGGGAAGTGGATCAGGCCTCCCTCTGCTGGTCCAAAG GACAATAGCTAAGCAAATTCAGATGGTGAAGCAGATTGGAAAAGGCCGCTATGGCGAGGTGTGGATGGGA AAGTGGCGTGGAGAAAAGGTGGCTGTGAAAGTGTTCTTCACCACGGAGGAAGCCAGCTGGTTCCGAGAGA CTGAGATATATCAGACGGTCCTGATGCGGCATGAGAATATTCTGGGGTTCATTGCTGCAGATATCAAAGG GACTGGGTCCTGGACTCAGTTGTACCTCATCACAGACTATCATGAAAACGGCTCCCTTTATGACTATCTG AAATCCACCACCTTAGACGCAAAGTCCATGCTGAAGCTAGCCTACTCCTCTGTCAGCGGCCTATGCCATT TACACACGGAAATCTTTAGCACTCAAGGCAAGCCAGCAATCGCCCATCGAGACTTGAAAAGTAAAAACAT CCTGGTGAAGAAAAATGGAACTTGCTGCATAGCAGACCTGGGCTTGGCTGTCAAGTTCATTAGTGACACA AATGAGGTTGACATCCCACCCAACACCCGGGTTGGCACCAAGCGCTATATGCCTCCAGAAGTGCTGGACG AGAGCTTGAATAGAAACCATTTCCAGTCCTACATTATGGCTGACATGTACAGCTTTGGACTCATCCTCTG GGAGATTGCAAGGAGATGTGTTTCTGGAGGTATAGTGGAAGAATACCAGCTTCCCTATCACGACCTGGTG CCCAGTGACCCTTCTTATGAGGACATGAGAGAAATTGTGTGCATGAAGAAGTTACGGCCTTCATTCCCCA ATCGATGGAGCAGTGATGAGTGTCTCAGGCAGATGGGGAAGCTTATGACAGAGTGCTGGGCGCAGAATCC TGCCTCCAGGCTGACGGCCCTGAGAGTTAAGAAAACCCTTGCCAAAATGTCAGAGTCCCAGGACATTAAA CTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230201 representing NM_001277220 Red=Cloning site Green=Tags(s) MWAPRRRMERVQPPPLGPRSYVVNATTTVRKTQSTISADGYCFTMIEEDDSGMPVVTSGCLGLEGSDFQC RDTPIPHQRRSIECCTERNECNKDLHPTLPPLKDRDFVDGPIHHKALLISVTVCSLLLVLIILFCYFRYK RQEARPRYSIGLEQDETYIPPGESLRDLIEQSQSSGSGSGLPLLVQRTIAKQIQMVKQIGKGRYGEVWMG KWRGEKVAVKVFFTTEEASWFRETEIYQTVLMRHENILGFIAADIKGTGSWTQLYLITDYHENGSLYDYL KSTTLDAKSMLKLAYSSVSGLCHLHTEIFSTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVKFISDT NEVDIPPNTRVGTKRYMPPEVLDESLNRNHFQSYIMADMYSFGLILWEIARRCVSGGIVEEYQLPYHDLV PSDPSYEDMREIVCMKKLRPSFPNRWSSDECLRQMGKLMTECWAQNPASRLTALRVKKTLAKMSESQDIK L TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277220
ORF Size	1473 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277220.1, NP_001264149.1
RefSeq Size	5357 bp
RefSeq ORF	1476 bp
Locus ID	12167
UniProt ID	P36898
MW	56.3 kDa
Gene Summary	This gene encodes a serine/threonine kinase that functions as a receptor for bone morphogenetic proteins (BMPs). The encoded protein is a type I receptor, and forms a complex of two type II and two type I receptors at the cell membrane. This complex signals downstream to activate SMAD transcriptional regulators. This signaling is important in skeletal and bone development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

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