Dach2 (NM_001289733) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9430028N04Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229949 representing NM_001289733 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAGGAAACAAGCTGTTAACAGTTCAAGGCCTGGCAGGCCCCCTAAGCGTTCTTTGGGAGTATTGC AAGATAATGCCCGACTTCTGCCCCATGCAGTCCCAGGCCTCTTATCACCAGGACTGATCACTCCAACAGG TATCACAGCTGCAGCGATGGCTGAGGCCATGAAGCTCCAGAAGATGAAGCTTATGGCAATGAATACTCTT CAGGGTAATGGAAGCCAAAATGGGACTGAGTCAGAGCCAGATGACCTTAATTCTACGACAGGTGGAAGTG AATCCTCCTGGGATAAAGATAAGATACAATCTCCACTTGCTGCTTCTGGACCTCAACATGGAATTGCTCA TGCAGCACTTGCTGGTCAGCCAGGCCTTGGAGGTGCTCCGACCCTTAATCCACTTCAGCAGAATCACCTG CTAAGCAATCGTCTGGATCTCCCATTTATGATGATGCCTCATCCCCTACTTCCAGTCAGCTTACCTCCTG CATCAGTTGCCATGGCAATGAATCAGATGAATCATCTCAATACTATTGCCAACATGGCTGCTGCAGCCCA GATTCACAGTCCACTCTCCAGAGCTGGTGCCTCTGTTATAAAGGAACGGATCCCAGAGAGTCCTTCTCCT GCTCCCTCTCTGGAAGAGAGTCATCGTCCTGGGAGCCAGACCTCCTCCCACCCAAGCAGCAGTGTGTCCA GCTCTCCCTCGCAGATGGATCATCATTCAGAGAGAATGGTTATGATGCCCAACAATCGAGAAGAGCTTAT TGTTGACCAAGATAATGGACAAAGCATAAAAAAATTCCAGAGGGATAATAAAGAAGAAGTACCAGCTCAA ATCCCAGTCATGAAGTCACCCTTGGATAAGATCCAGTTGGCTCCTGGACAGGCATTGCATCCTGGATTCC CTGGACCATTCATTTTTGCAGATAGTCTATCTTCTGTGGAGACTCTGTTGACCAACATTCAGGGTCTACT GAAAGTGGCTTTGGATAATGCTCGTATCCAGGAGAAGCAGATTCAGCAGGAAAAGAAGGAACTGCGAATA GAGCTCTTCAGAGAAAGAGAAATTAGAGAAAACCTGGAGCGACAACTTGCAGTTGAGCTTCAAAGCAGAA GTACAATGCAAAAGCGTCTGAAAAAAGAGAAAAAGGCTAAGAGAAAACTTCAGGAAGCCTTGGAATTTGA ATCAAAGCGCCGCGAGCAAGTGGAGCAAGCACTTAAACAAGCTACATCTGGTGACAGTGGACTGAGAATG TTAAAAGATTCTGGAATTCCAGATATTGAAATAGAAAACAGTGGAACGCCGCATGACAGTGCTGCTATGC AAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229949 representing NM_001289733 Red=Cloning site Green=Tags(s) MTRKQAVNSSRPGRPPKRSLGVLQDNARLLPHAVPGLLSPGLITPTGITAAAMAEAMKLQKMKLMAMNTL QGNGSQNGTESEPDDLNSTTGGSESSWDKDKIQSPLAASGPQHGIAHAALAGQPGLGGAPTLNPLQQNHL LSNRLDLPFMMMPHPLLPVSLPPASVAMAMNQMNHLNTIANMAAAAQIHSPLSRAGASVIKERIPESPSP APSLEESHRPGSQTSSHPSSSVSSSPSQMDHHSERMVMMPNNREELIVDQDNGQSIKKFQRDNKEEVPAQ IPVMKSPLDKIQLAPGQALHPGFPGPFIFADSLSSVETLLTNIQGLLKVALDNARIQEKQIQQEKKELRI ELFREREIRENLERQLAVELQSRSTMQKRLKKEKKAKRKLQEALEFESKRREQVEQALKQATSGDSGLRM LKDSGIPDIEIENSGTPHDSAAMQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289733
ORF Size	1335 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289733.1, NP_001276662.1
RefSeq Size	5708 bp
RefSeq ORF	1338 bp
Locus ID	93837
UniProt ID	Q925Q8
MW	48.9 kDa
Gene Summary	Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain.[UniProtKB/Swiss-Prot Function]

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