Cacng5 (NM_001199301) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228935 representing NM_001199301 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCCTGTGGGAGGAAGGCCCTGACCCTGCTGAGCAGCGTCTTTGCCGTCTGCGGCCTGGGGCTGC TGGGTATCGCGGTCAGCACGGACTACTGGCTCTACCTGGAAGAAGGCATAATCCTCCCACAGAACCAGAG CACAGAGGTCAAGATGTCCCTGCACTCGGGCCTCTGGCGGGTCTGCTTCCTCGCTGGTGAGGAGCGAGGA CGCTGTTTTACCATAGAGTATGTGATGCCCATGAACTCCCAGATGACGTCAGAATCCACTGTCAATGTTC TAAAAATGATTCGCTCAGCCACGCCGTTCCCCCTCGTCAGCCTCTTCTTCATGTTCATTGGGTTTATCCT GAGCAACATCGGGCACATCCGTCCGCACCGGACCATACTGGCCTTTGTCTCCGGCATCTTCTTTATCCTC TCTGGCCTCTCTCTTGTGGTGGGCCTGGTGCTGTACATCTCCAGCATCAATGACGAGATGCTCAACAGAA CCAAGGATGCAGAGACCTATTTCAACTACAAGTATGGATGGTCATTTGCCTTTGCTGCCATCTCCTTCCT TTTAACAGAGAGTGCTGGGGTGATGTCTGTGTACCTGTTCATGAAGAGGTACACTGCTGAGGACATGTAC AGACCTCACCCAGGCTTCTACCGGCCACGTCTGAGCAACTGCTCCGATTACTCTGGCCAGTTTCTACATC CAGATGCCTGGATCCGGGGCCGCAGCCCCTCGGACATCTCCAGCGACGCCTCGCTACAGATGAACAGCAA CTACCCAGCTTTGCTCAAGTGCCCAGACTATGACCAGATGTCATCATCTCCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228935 representing NM_001199301 Red=Cloning site Green=Tags(s) MSACGRKALTLLSSVFAVCGLGLLGIAVSTDYWLYLEEGIILPQNQSTEVKMSLHSGLWRVCFLAGEERG RCFTIEYVMPMNSQMTSESTVNVLKMIRSATPFPLVSLFFMFIGFILSNIGHIRPHRTILAFVSGIFFIL SGLSLVVGLVLYISSINDEMLNRTKDAETYFNYKYGWSFAFAAISFLLTESAGVMSVYLFMKRYTAEDMY RPHPGFYRPRLSNCSDYSGQFLHPDAWIRGRSPSDISSDASLQMNSNYPALLKCPDYDQMSSSPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199301
ORF Size	825 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199301.1, NP_001186230.1
RefSeq Size	3771 bp
RefSeq ORF	828 bp
Locus ID	140723
UniProt ID	Q8VHW4
MW	30.9 kDa
Gene Summary	Regulates the gating properties of AMPA-selective glutamate receptors (AMPARs). Modulates their gating properties by accelerating their rates of activation, deactivation and desensitization. Displays subunit-specific AMPA receptor regulation. Shows specificity for GRIA1, GRIA4 and the long isoform of GRIA2. Thought to stabilize the calcium channel in an inactivated (closed) state (By similarity).[UniProtKB/Swiss-Prot Function]

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