Dicer1 (NM_148948) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110006F08Rik; D12Ertd7e; mKIAA0928
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227139 representing NM_148948 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAATGGGTCCTTTCTTTGGACTGCCATGGCAAC AAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATACCAGGTTGAGCTGCTGGAAGCAGCTCTGGA CCATAACACAATTGTGTGCTTGAACACTGGCTCAGGGAAGACGTTCATCGCGGTCCTGCTCACCAAAGAG CTGGCCCATCAGATCAGGGGCGACCTCAACCCGCATGCAAAAAGGACCGTGTTCCTCGTCAACTCTGCAA ACCAGGTTGCTCAACAAGTGTCAGCCGTCAGAACTCACTCGGATCTCAAGGTTGGAGAGTACTCAGACCT AGAAGTAAATGCATCTTGGACAAAAGAGAGATGGAGCCAAGAGTTTACTAAGCACCAGGTTCTCATTATG ACTTGCTATGTCGCCTTGACTGTTTTGAAAAATGGTTACTTATCACTGTCAGACATTAACCTTTTGGTGT TTGATGAGTGTCATCTTGCGATTCTAGACCACCCCTATCGTGAAATTATGAAGCTCTGTGAGAGTTGTCC ATCATGCCCTCGAATTTTGGGACTAACTGCTTCCATTTTAAATGGGAAATGTGATCCAGAGGAATTGGAA GAGAAGATTCAGAAACTGGAGCGAATTCTCAGGAGTGACGCTGAGACGGCGACTGACTTGGTGGTCCTGG ACAGATACACTTCTCAGCCTTGTGAGATTGTGGTAGACTGTGGACCGTTTACTGACAGAAGTGGGCTGTA TGAGAGATTGCTGATGGAGTTAGAAGCAGCACTTGATTTTATCAATGATTGTAATGTAGCTGTACATTCA AAGGAAAGAGATTCTACTTTGATTTCCAAACAAATCCTCTCAGACTGCCGTGCAGTGTTGGTTGTTCTGG GACCCTGGTGTGCAGATAAAGTAGCTGGGATGATGGTAAGAGAACTTCAGAAGTACATCAAACATGAGCA AGAGGAGCTACACAGGAAGTTCCTATTGTTTACAGACACCTTGTTAAGGAAAATACACGCCCTGTGTGAA GAGTACTTCTCACCTGCCTCACTTGACCTGAAGTATGTAACTCCCAAAGTCATGAAACTGCTCGAGATCC TGCGCAAATACAAGCCCTATGAGCGACAGCAGTTTGAAAGTGTTGAGTGGTACAATAACAGGAATCAGGA TAATTACGTGTCCTGGAGTGATTCCGAGGATGACGATGATGACGAAGAAATTGAAGAAAAAGAAAAGCCA GAGACAAATTTTCCTTCTCCGTTTACCAATATTTTATGTGGGATTATTTTTGTGGAAAGAAGATACACAG CAGTTGTCCTAAACAGATTGATAAAGGAAGCTGGCAAACAAGATCCAGAGCTGGCTTATATCAGCAGCAA CTTTATAACTGGACACGGCATTGGAAAGAACCAGCCTCGCAGCAAACAGATGGAGGCGGAGTTCAGAAAG CAGGAGGAGGTACTTAGGAAATTTCGAGCTCATGAGACCAACCTGCTCATTGCAACAAGTGTTGTAGAAG AGGGTGTGGATATTCCCAAGTGCAATTTGGTGGTTCGTTTTGATTTGCCTACAGAGTATCGATCATATGT CCAGTCTAAAGGACGGGCACGAGCGCCGATCTCTAATTACGTAATGTTAGCAGATACAGACAAGATAAAG AGCTTTGAAGAAGACCTCAAGACCTACAAAGCTATTGAAAAGATCTTAAGGAACAAGTGTTCGAAGTCAG CGGATGGTGCTGAGGCGGACGTCCATGCTGGTGTAGATGATGAGGATGCTTTCCCGCCATATGTGCTGAG GCCTGATGATGGGGGTCCTCGAGTCACAATCAACACTGCCATTGGACACATCAACAGATATTGTGCTCGA TTACCAAGTGATCCGTTTACGCATCTAGCTCCCAAATGTAGAACCCGAGAATTGCCTGATGGTACATTTT ATTCAACTCTTTATCTTCCGATTAACTCACCTCTTCGAGCCTCCATTGTTGGTCCACCGATGGACAGTGT ACGGTTGGCTGAAAGAGTGGTAGCTCTCATTTGCTGTGAAAAACTGCACAAAATTGGTGAACTGGATGAA CACTTGATGCCAGTTGGGAAAGAGACTGTTAAATATGAAGAAGAGCTGGACTTACATGACGAGGAGGAGA CCAGTGTTCCCGGGAGACCAGGGTCCACAAAACGACGGCAGTGCTACCCCAAAGCAATTCCAGAGTGCTT GAGGGAGAGTTACCCCAAACCCGACCAGCCTTGTTACCTGTATGTGATAGGAATGGTCTTAACTACCCCC CTCCCTGATGAACTCAACTTCAGGCGGCGGAAGCTCTACCCACCTGAGGACACCACAAGATGCTTTGGGA TCCTGACAGCCAAACCCATACCTCAGATTCCTCACTTTCCTGTGTATACACGCTCTGGAGAGGTCACCAT ATCCATCGAGCTGAAGAAGTCTGGTTTCACATTGTCTCAACAAATGCTCGAGCTGATTACAAGATTGCAC CAGTACATATTCTCACATATTCTCCGGCTTGAGAAGCCTGCCCTGGAGTTTAAACCTACAGGCGCGGAGT CAGCCTACTGTGTTCTACCTCTTAATGTTGTTAATGACTCCGGCACTTTGGACATTGACTTTAAATTCAT GGAGGATATTGAGAAGTCGGAAGCTCGGATAGGCATTCCTAGCACCAAGTATTCAAAGGAAACACCGTTT GTCTTTAAGTTAGAAGATTACCAAGATGCAGTGATCATTCCAAGATACCGCAATTTTGATCAGCCTCATC GATTTTATGTAGCTGATGTATATACGGATCTTACCCCACTCAGTAAATTTCCTTCCCCTGAATATGAAAC TTTTGCAGAATATTATAAAACAAAGTATAACCTTGACCTGACCAATCTCAACCAGCCACTGCTGGATGTT GACCACACATCTTCCAGACTGAACCTTTTGACACCTCGGCATTTGAATCAGAAGGGGAAAGCACTTCCTC TAAGCAGTGCTGAGAAGAGGAAGGCCAAATGGGAAAGTCTGCAGAACAAACAGATCCTGGTTCCGGAACT CTGTGCTATACATCCAATCCCAGCATCACTGTGGAGAAAAGCGGTTTGTCTCCCCAGCATACTGTATCGC CTTCACTGCCTTCTGACTGCAGAGGAACTAAGAGCTCAGACAGCCAGCGATGCTGGTGTGGGTGTCAGAT CACTTCCCGTGGATTTTAGATACCCTAACTTAGACTTCGGGTGGAAAAAATCTATTGATAGCAAGTCTTT CATCTCAAGCTGTAACTCCTCGTTGGCTGAGAGTGATAATTACTGTAAGCACAGCACAACTGTAGTCCCT GAACATGCTGCACATCAAGGTGCTACTAGACCCTCCTTAGAAAACCATGACCAAATGTCTGTGAACTGCA AAAGGTTGCCCGCTGAGTCACCTGCTAAGCTCCAAAGTGAGGTTTCAACGGATCTTACAGCAATTAATGG TCTTTCTTACAATAAAAACCTTGCCAATGGCAGTTATGATTTAGTTAACAGAGACTTTTGCCAAGGAAAT CAGCTGAATTACTTCAAGCAGGAAATACCTGTACAACCAACTACCTCATATCCCATTCAGAATTTGTACA ATTATGAGAACCAGCCCAAGCCCAGCAATGAATGTCCTCTACTGAGTAATACGTACCTTGATGGGAACGC TAACACATCTACCTCAGATGGAAGCCCCGCGGTGTCCACGATGCCTGCCATGATGAATGCTGTTAAAGCA CTCAAGGACAGGATGGACTCTGAGCAGAGCCCTTCTGTTGGGTACTCCTCAAGGACTCTTGGCCCCAATC CGGGACTGATTCTCCAGGCTTTGACACTGTCAAATGCCAGTGATGGGTTTAACCTGGAGCGGCTTGAGAT GCTTGGCGACTCCTTTTTAAAGCATGCTATCACCACATATCTGTTTTGCACGTACCCTGATGCTCATGAA GGCCGCCTTTCATATATGAGAAGCAAAAAGGTCAGCAACTGTAACCTGTATCGCCTTGGCAAGAAGAAGG GACTGCCCAGCCGCATGGTGGTGTCGATATTCGATCCTCCTGTGAATTGGCTTCCTCCTGGTTATGTGGT AAACCAAGACAAAAGTAACTCAGAGAAGTGGGAAAAGGATGAAATGACAAAAGACTGCTTGCTGGCTAAT GGCAAACTGGGTGAGGCCTGTGAGGAGGAGGAGGACCTGACGTGGAGGGCCCCCAAGGAGGAGGCCGAGG ATGAGGACGACTTCCTGGAGTATGACCAGGAGCATATCCAGTTTATAGACAGCATGCTCATGGGCTCGGG CGCCTTCGTGAGGAAAATCTCTCTGTCGCCCTTCTCAGCCTCCGACTCTGCATACGAATGGAAGATGCCC AAGAAGGCCTCCTTGGGCAGTATGCCGTTTGCCTCGGGCCTTGAGGATTTTGATTACAGCTCTTGGGATG CGATGTGCTATCTGGATCCTAGCAAAGCCGTTGAAGAGGATGACTTTGTGGTAGGTTTCTGGAATCCATC AGAAGAGAACTGTGGCGTCGACACAGGGAAGCAGTCCATTTCTTACGACTTGCACACGGAGCAGTGCATT GCTGACAAGAGCATAGCGGACTGTGTGGAGGCCCTGCTGGGCTGCTACTTAACCAGCTGCGGCGAGAGGG CTGCTCAGCTCTTCCTCTGTTCGCTGGGGCTGAAGGTGCTCCCAGTAATCAAAAGGACCAGCCGGGAGAA GGCCTTGGACCCTGCTCAGGAGAACGGAAGCAGCCAACAAAAGAGCCTTTCAGGGAGCTGTGCCTCTCCA GTTGGCCCACGCTCCTCTGCAGGGAAAGACTTGGAGTACGGCTGCCTGAAGATCCCACCGAGGTGCATGT TTGACCATCCAGACGCAGAGAAAACCCTCAACCACCTGATATCTGGGTTTGAAACTTTTGAGAAGAAAAT TAACTATAGATTCAAGAATAAGGCTTATCTTCTGCAGGCTTTTACACACGCCTCCTACCACTACAACACT ATCACTGATTGTTACCAGCGCTTAGAATTCCTGGGAGATGCGATTTTGGACTACCTCATAACCAAGCACC TTTATGAAGACCCACGGCAGCATTCTCCAGGGGTCTTGACTGACTTGCGCTCTGCCCTTGTCAATAACAC CATCTTTGCATCATTGGCTGTGAAGTATGACTACCACAAGTACTTTAAAGCTGTCTCTCCTGAGCTCTTT CATGTTATTGATGACTTTGTGAAGTTTCAGCTTGAGAAGAACGAAATGCAAGGAATGGACTCTGAGCTTA GGAGATCCGAGGAGGATGAGGAGAAAGAAGAGGATATCGAAGTCCCCAAGGCCATGGGGGACATTTTTGA GTCTCTTGCTGGTGCCATTTATATGGACAGTGGGATGTCTCTGGAGGTGGTCTGGCAGGTGTACTATCCG ATGATGCAGCCTCTAATAGAAAAGTTTTCTGCAAATGTGCCTCGTTCTCCAGTGAGAGAATTGCTCGAGA TGGAACCAGAAACTGCCAAGTTTAGCCCAGCGGAGAGAACTTATGATGGAAAGGTCAGAGTCACCGTGGA AGTGGTGGGAAAGGGGAAATTTAAAGGTGTTGGCCGGAGCTACAGGATCGCCAAGTCTGCTGCAGCACGA AGGGCCCTCCGCAGCCTCAAAGCTAACCAGCCTCAGGTTCCTAACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227139 representing NM_148948 Red=Cloning site Green=Tags(s) MAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKE LAHQIRGDLNPHAKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSDLEVNASWTKERWSQEFTKHQVLIM TCYVALTVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCESCPSCPRILGLTASILNGKCDPEELE EKIQKLERILRSDAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEAALDFINDCNVAVHS KERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTLLRKIHALCE EYFSPASLDLKYVTPKVMKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDDDEEIEEKEKP ETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRSKQMEAEFRK QEEVLRKFRAHETNLLIATSVVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIK SFEEDLKTYKAIEKILRNKCSKSADGAEADVHAGVDDEDAFPPYVLRPDDGGPRVTINTAIGHINRYCAR LPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMDSVRLAERVVALICCEKLHKIGELDE HLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPKPDQPCYLYVIGMVLTTP LPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSQQMLELITRLH QYIFSHILRLEKPALEFKPTGAESAYCVLPLNVVNDSGTLDIDFKFMEDIEKSEARIGIPSTKYSKETPF VFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDV DHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYR LHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISSCNSSLAESDNYCKHSTTVVP EHAAHQGATRPSLENHDQMSVNCKRLPAESPAKLQSEVSTDLTAINGLSYNKNLANGSYDLVNRDFCQGN QLNYFKQEIPVQPTTSYPIQNLYNYENQPKPSNECPLLSNTYLDGNANTSTSDGSPAVSTMPAMMNAVKA LKDRMDSEQSPSVGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHE GRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNSEKWEKDEMTKDCLLAN GKLGEACEEEEDLTWRAPKEEAEDEDDFLEYDQEHIQFIDSMLMGSGAFVRKISLSPFSASDSAYEWKMP KKASLGSMPFASGLEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCI ADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTSREKALDPAQENGSSQQKSLSGSCASP VGPRSSAGKDLEYGCLKIPPRCMFDHPDAEKTLNHLISGFETFEKKINYRFKNKAYLLQAFTHASYHYNT ITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELF HVIDDFVKFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEVVWQVYYP MMQPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAAR RALRSLKANQPQVPNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_148948
ORF Size	5718 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_148948.2, NP_683750.2
RefSeq Size	9851 bp
RefSeq ORF	5721 bp
Locus ID	192119
MW	216.2 kDa

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